本文選題：硫磺負(fù)離子 + 大鼠��； 參考：《延邊大學(xué)》2015年碩士論文

【摘要】：本試驗主要研究硫磺負(fù)離子飼料添加劑對大鼠的亞慢性毒性和對肝臟抗氧化能力的影響。選取清潔級SD大鼠80只,雌雄各半。隨機分成4組,每組雌雄各10只,分別為對照組,飼喂普通飼料；低劑量組,飼喂含有0.3%硫磺負(fù)離子飼料添加劑的飼料；中劑量組,飼喂含有0.9%硫磺負(fù)離子飼料添加劑的飼料；高劑量組,飼喂含有2.7%硫磺負(fù)離子飼料添加劑的飼料。連續(xù)喂養(yǎng)90d。第45天每組挑選6只大鼠,雌雄各3只,進行采血、剖檢、臟器稱重。第90d,所有大鼠全部采血、剖檢、臟器稱重、并將肝臟研磨測定丙二醛(MDA)、谷胱甘肽(GSH)、過氧化物歧化酶SOD比活力、對高劑量組大鼠腎臟作組織學(xué)觀察。試驗結(jié)果：(1)大鼠體重的變化：試驗期間各組動物的基本情況良好,無厭食、死亡和其它異常行為,通過每周對大鼠體重的記錄,發(fā)現(xiàn)雌性高劑量組在第28天和第49天時與對照組有顯著性差異(P0.05),其余各組與對照組相比較無顯著性差異。(2)飼料利用率：硫磺負(fù)離子飼料添加劑飼喂初期,試驗組飼料利用率明顯低于對照組,隨著試驗的進行,飼料利用率在不斷提高,到第六周試驗組的飼料利用率開始高于對照組。(3)臟器系數(shù)：試驗中期高劑量組雌性大鼠的肺的臟器系數(shù)高于對照組,并且差異顯著(p0.05),其余各組與對照組相比較均無顯著性差異；試驗?zāi)┢诘蛣┝拷M雄性大鼠的腎臟的臟器系數(shù)與對照組相比有顯著性差異(p0.05),中劑量組雌性大鼠胸腺的臟器系數(shù)與對照組相比有顯著性差異(p0.05),其余各試驗組與對照組相比較均無顯著性差異(4)血液學(xué)檢查結(jié)果：硫磺負(fù)離子90 d喂養(yǎng)試驗雌雄性大鼠中期和末期低、中、高三個劑量組的血液學(xué)檢查結(jié)果與對照組相比較均無顯著性差異。(5)血清生化檢查結(jié)果：試驗中期雌性低劑量組和高劑量組的谷草轉(zhuǎn)氨酶明顯偏高,與對照組比較差異極顯著(p0.01),雄性高劑量組堿性磷酸酶水平偏高,與對照組比較有顯著性差異(p0.05),；試驗?zāi)┢诖菩鄞笫蟾鱾�試驗組的血清學(xué)檢查結(jié)果與對照組相比均無顯著性差異。(6)組織學(xué)檢查結(jié)果：通過光學(xué)顯微鏡對組織切片的觀察,未發(fā)現(xiàn)組織有異常變化。(7)肝臟抗氧化能力：對大鼠肝臟中丙二醛和谷胱甘肽的含量沒有明顯的影響,與對照組相比較沒有產(chǎn)生顯著性的差異(p0.05)；對肝臟過氧化物歧化酶(SOD)比活力也有明顯的影響,試驗組各組的SOD比活力比對照組高,中劑量組有統(tǒng)計學(xué)差異,(p0.05)。本試驗結(jié)果表明硫磺負(fù)離子飼料添加劑喂養(yǎng)大鼠90d沒有產(chǎn)生毒性,并且能夠增強大鼠肝臟抗氧化能力,本試驗中的最適添加量為0.9%。
[Abstract]:The effects of sulfur anion feed additive on the subchronic toxicity and antioxidation ability of liver in rats were studied. Eighty SD rats of clean grade were selected, half male and half male. They were randomly divided into 4 groups: control group (n = 10), low dose group (fed with 0.3% sulfur negative ion), medium dose group (n = 10), and medium dose group (n = 10) fed with 0.9% sulfur negative ion feed additive. High dose group, feed containing 2.7% sulfur negative ion feed additive. Continuous feeding for 90 days. On the 45th day, 6 rats, male and female, were selected for blood collection, dissection and weighing of viscera. On the 90th day, all the rats were collected blood, dissected, weighed by viscera, and the specific activities of malondialdehyde (MDA), glutathione (GSH) and peroxidase dismutase (SOD) were measured by liver grinding. The kidney of high dose group was observed histologically. Results: changes in the body weight of rats: during the trial, the animals in each group were basically in good condition, free of anorexia, death and other abnormal behaviors, and recorded the weight of the rats on a weekly basis. It was found that there was significant difference between the female high dose group and the control group on the 28th and 49th day, but there was no significant difference between the other groups and the control group. The feed utilization rate of the test group was obviously lower than that of the control group. At the sixth week, the feed utilization ratio of the experimental group was higher than that of the control group. The organ coefficient of the female rats in the high dose group was higher than that in the control group, and the difference was significant (p 0.05). There was no significant difference between the other groups and the control group. At the end of the experiment, the kidney organ coefficient of the male rats in the low dose group was significantly different from that of the control group (p 0.05), the thymus organ coefficient of the female rats in the middle dose group was significantly different from that of the control group, and the other test groups were different from the control group. There was no significant difference between the two groups (4) the results of hematological examination showed that the middle and late stages of female and male rats fed with sulfur anion for 90 days were lower than those in the control group. There was no significant difference in serum biochemistry between the three dose groups and the control group. The results of serum biochemical examination showed that the glutamic oxaloacetic transaminase was significantly higher in the female low dose group and high dose group in the middle of the trial. Compared with the control group, the difference was significant (P 0.01), and the alkaline phosphatase level in the male high dose group was higher than that in the control group. At the end of the experiment, there was no significant difference in serological examination results between male and female rats compared with the control group. No abnormal changes were found in the tissue. 7) the antioxidant capacity of liver: there was no significant effect on the contents of malondialdehyde and glutathione in the liver of rats. Compared with the control group, there was no significant difference between the two groups (p0.05), and the specific activity of SOD in liver was also significantly affected. The specific activity of SOD in the experimental group was higher than that in the control group, and there was a significant difference in the middle dose group (P 0.05). The results showed that sulfur negative feed additive did not produce toxicity for 90 days and could enhance the anti-oxidation ability of rat liver. The optimum dosage was 0.9g in this experiment.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S859.82

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相關(guān)期刊論文 前1條

1 趙炳超;石波;李秀波;梁平;劉一峰;;我國飼料添加劑的現(xiàn)狀與發(fā)展趨勢[J];飼料與畜牧;2006年02期

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