本文選題：禽網(wǎng)狀內皮組織增生癥病毒 + 全基因組�。� 參考：《中國預防獸醫(yī)學報》2016年11期

【摘要】：為比較分析近年來禽網(wǎng)狀內皮組織增生癥病毒(REV)的基因組變異情況,本研究對分離自某父母代肉種雞場患病雞的REV LN1201株進行PCR擴增和克隆測序,獲得了LN1201株的前病毒全基因組序列,并將其與不同參考株基因組進行了比較分析。結果顯示,LN1201株前病毒基因組全長8 438 bp,具有典型復制完整型逆轉錄病毒的基因組結構。與參考株相比,LN1201與國內分離株HLJR0901和疫苗污染病毒株MD-2同源性較高,但其U3區(qū)與不同病毒株的同源性相對較低,并且在U3區(qū)出現(xiàn)了罕見的14 bp堿基插入,顯示該病毒株可能為LTR發(fā)生重組的病毒。本研究有助于了解我國雞群中REV的基因組特性,提示有必要繼續(xù)關注REV誘發(fā)的腫瘤及其基因變異。
[Abstract]:In order to compare and analyze the genomic variation of reticuloendotheliosis virus (Rev) in recent years, the REV LN1201 strain isolated from a parent broiler farm was amplified by PCR and cloned and sequenced. The whole genome sequence of LN1201 strain was obtained and compared with that of different reference strains. The results showed that the genome of LN1201 strain was 8 438 BP in length, and had the genome structure of typical replicating intact retrovirus. Compared with the reference strain, LN1201 has higher homology with domestic isolates HLJR0901 and vaccine-contaminated virus strain MD-2, but its U3 region is relatively low with different virus strains, and there is a rare 14bp nucleotide insertion in U3 region. This indicates that the virus strain may be a recombinant LTR virus. This study is helpful to understand the genomic characteristics of REV in chicken populations in China, suggesting that it is necessary to continue to pay attention to REV induced tumors and their gene variation.
【作者單位】： 山東農(nóng)業(yè)大學動物醫(yī)學院;
【基金】：農(nóng)業(yè)部財政專項一種禽場傳播性疾病疫情調查
【分類號】：S852.65
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本文編號：1892660