本文選題：苜蓿 + 谷草��； 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：本研究以空懷期小尾寒羊?yàn)樵囼?yàn)動(dòng)物,通過(guò)體外產(chǎn)氣法、尼龍袋法和飼養(yǎng)試驗(yàn)3種不同方法,研究苜蓿和谷草的組合效應(yīng),并初步探究產(chǎn)生組合效應(yīng)的作用機(jī)理,為合理利用谷草資源提供數(shù)據(jù)基礎(chǔ)。試驗(yàn)1.體外產(chǎn)氣法評(píng)定苜蓿和谷草的組合效應(yīng)本試驗(yàn)選取三只健康且安裝永久瘤胃瘺管的成年小尾寒羊母羊作為瘤胃液供體羊。將苜蓿(AH)和谷草(MS)分別以不同比例(AH:MS=100:0、AH:MS=0:100、AH:MS=75:25、AH:MS=50:50、AH:MS=25:75)組成粗飼料,并以精粗比40:60組成5種不同日糧(Ⅰ(A100M0)、Ⅱ(A0M100)、Ⅲ、(A75M25)Ⅳ、(A50M50)Ⅴ(A25M75))作為發(fā)酵底物,進(jìn)行體外產(chǎn)氣試驗(yàn)。記錄發(fā)酵2、4、8、12、24、36和48 h累積產(chǎn)氣量(GP),并測(cè)定發(fā)酵12、24和48 h后培養(yǎng)液發(fā)酵參數(shù)—pH、氨態(tài)氮(NH3-N)、微生物蛋白(MCP)和揮發(fā)性脂肪酸(VFA)生成量。以發(fā)酵24 h的GP和MCP產(chǎn)量為指標(biāo)分別計(jì)算各日糧組合效應(yīng)指數(shù),以pH、VFA和NH3-N生成量的變化規(guī)律揭示組合效應(yīng)的機(jī)理。結(jié)果表明:(1)各組合的GP、GP動(dòng)力學(xué)參數(shù)、MCP、NH3-N和總揮發(fā)性脂肪酸(TVFA)生成量均隨日糧中苜蓿比例的增加顯著提高(P0.05);各組合不同時(shí)間點(diǎn)pH均在6.8~7.0之間,同一時(shí)間點(diǎn)各組pH差異不顯著(P0.05);各組TVFA和乙酸產(chǎn)量隨日糧中苜蓿比例增加而顯著提高(P0.05),但各組同一時(shí)間點(diǎn)乙酸/丙酸值差異不顯著(P0.05)。(2)以24小時(shí)GP為指標(biāo)評(píng)定各日糧組合效應(yīng)指數(shù)時(shí),Ⅲ、Ⅳ、Ⅴ三組的組合效應(yīng)指數(shù)分別為4.6%、7.5%和6.9%。(3)以24小時(shí)MCP產(chǎn)量為指標(biāo)評(píng)價(jià)各組組合效應(yīng)指數(shù)時(shí),Ⅲ、Ⅳ、Ⅴ三組的組合效應(yīng)指數(shù)分別為2.4%、13.4%和13.7%。試驗(yàn)2.尼龍袋法評(píng)定苜蓿和谷草的組合效應(yīng)本研究以5只安裝永久瘤胃瘺管的成年小尾寒羊母羊作為試驗(yàn)動(dòng)物,按照5×5拉丁方試驗(yàn)設(shè)計(jì),采用尼龍袋法測(cè)定試驗(yàn)1設(shè)計(jì)的5種日糧(Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ)主要營(yíng)養(yǎng)成分的瘤胃降解率;以粗蛋白(CP)和干物質(zhì)(DM)瘤胃有效降解率EDCP和EDDM作為計(jì)算組合效應(yīng)指數(shù)的主要觀(guān)察測(cè)定指標(biāo),計(jì)算組合效應(yīng)指數(shù);同時(shí)將中性洗滌纖維(NDF)和酸性洗滌纖維(ADF)瘤胃有效降解率EDNDF和EDADF作為對(duì)EDDM進(jìn)行剖分的二級(jí)觀(guān)察指標(biāo),揭示組合效應(yīng)的發(fā)生機(jī)理。結(jié)果表明:(1)各組EDDM、EDCP、EDNDF和EDADF均隨組合中苜蓿比例的增加而顯著提高(P0.05)。(2)以EDDM為指標(biāo)評(píng)定各日糧的組合效應(yīng)時(shí),Ⅲ、Ⅳ和Ⅴ三組的組合效應(yīng)指數(shù)分別為0.8%,4.0%,3.7%;以EDCP為指標(biāo)評(píng)定各日糧的組合效應(yīng)時(shí),Ⅲ、Ⅳ、Ⅴ三組的組合效應(yīng)指數(shù)分別為2.5%,3.5%,3.1%,Ⅳ組基于EDDM和EDCP計(jì)算的組合效應(yīng)指數(shù)均大于Ⅲ組和Ⅴ組,效果最好。(3)Ⅲ、Ⅳ、Ⅴ三種日糧中CF(粗纖維)對(duì)DM組合效應(yīng)指數(shù)的貢獻(xiàn)率分別為101.55%,68.75%,55.41%。試驗(yàn)3.飼養(yǎng)試驗(yàn)評(píng)定苜蓿和谷草的組合效應(yīng)——苜蓿和谷草不同比例飼喂對(duì)小尾寒羊日糧能量和粗蛋白轉(zhuǎn)化效率的影響試驗(yàn)選取60只空懷期小尾寒羊?yàn)樵囼?yàn)動(dòng)物,隨機(jī)將其分為四組(每組3個(gè)重復(fù),每個(gè)重復(fù)5只羊),分別飼喂由試驗(yàn)1和試驗(yàn)2優(yōu)選的Ⅰ、Ⅱ、Ⅳ和Ⅴ組日糧,進(jìn)行為期40天的飼養(yǎng)試驗(yàn)(預(yù)飼期10天)。飼養(yǎng)試驗(yàn)結(jié)束后從每個(gè)重復(fù)隨機(jī)挑選一只羊進(jìn)行為期7天(預(yù)飼期4天)消化代謝試驗(yàn)。以增重能量比(RGE)和增重蛋白比(RGP)為指標(biāo)計(jì)算各組日糧的組合效應(yīng)指數(shù),驗(yàn)證試驗(yàn)1和試驗(yàn)2的結(jié)論。結(jié)果表明:(1)試驗(yàn)羊的采食量和日增重隨著日糧中苜蓿比例的增加而增加,Ⅰ、Ⅳ、Ⅴ組干物質(zhì)采食量較Ⅱ組分別提高8.96%,6.39%,7.91%,差異顯著(P0.05);Ⅰ、Ⅳ組日增重較Ⅱ組分別提高51.46%、35.89%,差異顯著(P0.05),Ⅴ組日增重較Ⅱ組提高21.36%,差異不顯著(P0.05)。(2)以RGE指標(biāo)計(jì)算的Ⅳ、Ⅴ組的組合效應(yīng)指數(shù)分別為7.5%、3.5%。(3)以RGP指標(biāo)計(jì)算的Ⅳ、Ⅴ組的組合效應(yīng)指數(shù)分別為4.5%、3.7%�；谏鲜鲅芯�,可獲得以下結(jié)論:(1)體外產(chǎn)氣法、尼龍袋法和飼養(yǎng)試驗(yàn)所獲得的苜蓿與谷草不同比例組合后的組合效應(yīng)指數(shù)存在差異。(2)依據(jù)3種評(píng)定方法所得不同飼料組合效應(yīng)指數(shù)的排序結(jié)果基本一致。(3)以谷草為主要粗飼料的日糧中添加25%~50%苜蓿能取得較好的正組合效應(yīng),其中谷草和苜蓿以50:50組合可在能量轉(zhuǎn)化效率和蛋白質(zhì)轉(zhuǎn)化效率方面同時(shí)取得最大的正組合效應(yīng)。(4)依據(jù)組合效應(yīng)指數(shù)與動(dòng)物生產(chǎn)試驗(yàn)結(jié)果的一致性作為評(píng)判指標(biāo),尼龍袋法評(píng)定的準(zhǔn)確性高于體外產(chǎn)氣法,使用尼龍袋法所得結(jié)論更接近動(dòng)物生產(chǎn)試驗(yàn)所得結(jié)論。
[Abstract]:In this study, small tail Han sheep were taken as experimental animals, and the combined effects of alfalfa and grain grass were studied by 3 different methods in vitro gas production, nylon bag method and feeding test, and the mechanism of combination effect was preliminarily explored to provide a data basis for rational utilization of grain grass resources. Test 1. in vitro gas production method was used to assess alfalfa and millet grass group. Three healthy adult small tailed sheep ewes with permanent rumen fistula were selected as the donor sheep of the tumor gastric juice. AH and MS were composed of AH:MS=100:0, AH:MS=0:100, AH:MS=75:25, AH:MS=50:50, AH:MS=25:75, respectively, and 5 different diets (A100M0), II (A) were made up of 40:60 (A). 0M100), III, (A75M25) IV, (A50M50) V (A25M75)) as a fermentation substrate, conduct an in vitro gas production test. Record the fermentation 2,4,8,12,24,36 and 48 h cumulative gas yield (GP), and determine the fermentation parameters of the fermentation 12,24 and 48 h after the fermentation parameters - pH, ammonia nitrogen (NH3-N), microbiological protein (MCP) and volatile fatty acids. The combination effect index of each diet was calculated and the mechanism of combination effect was revealed by the variation of pH, VFA and NH3-N. The results showed that: (1) the formation of GP, GP dynamics, MCP, NH3-N and total volatile fatty acid (TVFA) in each combination increased significantly with the increase of Alfalfa ratio in the diet (P0.05); each combination of different time points was p. The difference between H and pH was not significant (P0.05) at the same time point (P0.05). The yield of TVFA and acetic acid in each group increased significantly with the ratio of Alfalfa in the diet (P0.05), but there was no significant difference in the value of acetic acid / propionic acid at the same time point (P0.05). (2) the combination effect of each diet was evaluated with 24 hour GP as the index, and the combination effect of group III, IV, and V three According to the index of 4.6%, 7.5% and 6.9%. (3) to evaluate the combination effect index of 24 hours MCP, the combination effect index of group III, IV, and V three was 2.4%, 13.4% and 13.7%. test 2. were used to evaluate the combination effect of alfalfa and grain grass with 5 adult small tail sheep ewes with permanent rumen fistula. A 5 * 5 Latin square test was designed to determine the ruminal degradation rate of the 5 diets (I, II, III, IV, V) of the 5 diet (I, II, III, IV, V) by nylon bag method, and the effective degradation rate of CP and DM in the rumen of the crude protein (CP) and dry matter (DM) was used as the main index to calculate the combination effect index, and the combination effect index was calculated. At the same time, the effective degradation rate of neutral detergent fiber (NDF) and acid detergent fiber (ADF), EDNDF and EDADF, was used as the two level observation index of EDDM, and the mechanism of combination effect was revealed. The results showed that: (1) EDDM, EDCP, EDNDF and EDADF all increased significantly with the increase of Alfalfa ratio in the combination (P0.05). (2) EDDM as the index evaluation. The combination effect index of group III, IV and V three groups was 0.8%, 4%, 3.7%, respectively. When EDCP was used to evaluate the combination effect of each diet, the combination effect index of group III, IV, and V three were 2.5%, 3.5%, 3.1% respectively. The combination effect index based on EDDM and EDCP in group IV were all larger than group III and group v. (3). The contribution rate of CF (coarse fiber) to the combination effect index of DM in three kinds of diets was 101.55%, 68.75%, and 68.75%, respectively, to evaluate the combination effect of alfalfa and millet straw test 3. - the effects of alfalfa and millet straw on the diet energy and the conversion efficiency of crude protein in the Small Tail Han sheep were tested in 60 empty Small Tail Han Sheep For experimental animals, they were randomly divided into four groups (3 repetitions in each group, 5 sheep for each repeat), feeding on the diet of group I, II, IV and V, selected by test 1 and test 2, respectively, for a 40 day feeding test (pre feeding period 10 days). After the feeding test, a sheep was selected for 7 days (4 days pre feeding) for the digestion and metabolism test. The combination of weight gain ratio (RGE) and weight gain ratio (RGP) was used to calculate the combination effect index of each diet, and the results of test 1 and test 2 were verified. The results showed that: (1) the feed intake and daily gain of the sheep increased with the increase of Alfalfa ratio in the diet, and the intake of dry matter in group I, IV and V increased by 8.96%, 6.39%, 7.91%, respectively. The difference was significant (P0.05). In group I, the daily gain of group IV was increased by 51.46%, 35.89%, and the difference was significant (P0.05). The daily gain of group V was 21.36% higher than that in group II (P0.05). (2) the combination effect index of group V was 7.5%, 3.5%. (3) was calculated with RGP index, and the combination effect index of group V was 4.5%, 3. respectively. 7%. based on the above research, we can obtain the following conclusions: (1) the combination effect index of alfalfa and millet straw obtained by the method of in vitro gas production, nylon bag method and feeding test is different. (2) the sequence results of different feed combination effect index obtained by 3 evaluation methods are basically the same. (3) the day of grain grass as the main roughage The best positive combination effect can be obtained by adding 25%~50% alfalfa in grain, in which the maximum positive combination effect can be obtained with the combination of grain and alfalfa with 50:50 combination in energy conversion efficiency and protein conversion efficiency. (4) according to the consistency of the combination effect index and the animal production test results as the evaluation index, the evaluation of the nylon bag method is accurate. The results obtained from nylon bag method are closer to the conclusion of animal production test.

【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S816

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 袁翠林;王利華;于子洋;朱亞駿;林英庭;;青貯玉米秸與4種羊常用粗飼料間的組合效應(yīng)研究[J];中國(guó)畜牧雜志;2014年15期

2 孫國(guó)強(qiáng);呂永艷;張杰杰;;利用體外瘤胃發(fā)酵法研究全株玉米青貯與花生蔓和羊草間的組合效應(yīng)[J];草業(yè)學(xué)報(bào);2014年03期

3 李豐成;;苜蓿飼喂雜交肉羊育肥效果試驗(yàn)[J];青海畜牧獸醫(yī)雜志;2014年02期

4 尹強(qiáng);格根圖;高靜;榮磊;賈玉山;;苜蓿干草與玉米秸稈混合飼草對(duì)羔羊育肥效果的影響[J];西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版);2013年06期

5 于騰飛;張杰杰;孫國(guó)強(qiáng);;花生蔓與4種粗飼料間組合效應(yīng)的研究[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2012年07期

6 崔占鴻;劉書(shū)杰;郝力壯;張曉衛(wèi);趙月平;;體外產(chǎn)氣法評(píng)價(jià)青海高原反芻家畜常用粗飼料組合效應(yīng)[J];草業(yè)科學(xué);2011年10期

7 戈新;王建華;張寶s，

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