本文選題：免疫增強(qiáng)劑 + 催乳保健散��； 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：目的:本研究利用免疫增強(qiáng)劑和催乳保健散預(yù)防圍產(chǎn)期奶牛的能量負(fù)平衡、免疫應(yīng)激及氧化應(yīng)激,探討免疫增強(qiáng)劑對圍產(chǎn)期奶牛免疫機(jī)能與抗氧化能力的影響,以及催乳保健散對圍產(chǎn)期奶牛產(chǎn)奶性能和營養(yǎng)物質(zhì)表觀消化率的影響,為今后進(jìn)一步研究免疫增強(qiáng)劑和催乳保健散對圍產(chǎn)期奶牛的作用機(jī)理奠定理論和試驗基礎(chǔ)。方法:試驗一:在圍產(chǎn)期奶牛日糧中添加免疫增強(qiáng)劑,并采集奶牛尾靜脈血液,使用試劑盒檢測血清中細(xì)胞因子(IL-2、IL-6、TNF-α)及免疫球蛋白(Ig A、Ig M、Ig G)含量。試驗二:在圍產(chǎn)期奶牛日糧中添加免疫增強(qiáng)劑,并采集奶牛尾靜脈血液,使用試劑盒檢測血清中抗氧化酶(GSH-Px、SOD、CAT、MDA)含量。試驗三:在圍產(chǎn)期奶牛日糧中添加催乳保健散,記錄奶牛產(chǎn)后3個月的產(chǎn)奶量,檢測奶樣中各成分(乳糖、乳蛋白、乳脂肪)含量,并計算4%乳脂校正乳量。試驗四:在圍產(chǎn)期奶牛日糧中添加催乳保健散,定時采集奶牛日糧和新鮮糞樣,檢測其中干物質(zhì)、粗蛋白、中性洗滌纖維及鈣、磷含量,計算奶牛的干物質(zhì)采食量和各營養(yǎng)物質(zhì)的表觀消化率。結(jié)果:試驗一:結(jié)果顯示:(1)免疫增強(qiáng)劑可以極顯著提高圍產(chǎn)期奶牛血清中Ig A、Ig M、Ig G含量(P0.01);(2)免疫增強(qiáng)劑可以極顯著提高血清中IL-6含量(P0.01),但對血清中TNF-α、IL-2含量的影響不穩(wěn)定。試驗二:結(jié)果顯示:免疫增強(qiáng)劑可以極顯著提高圍產(chǎn)期奶牛血清中SOD含量(P0.01),極顯著降低圍產(chǎn)期奶牛血清中MDA含量(P0.01),圍產(chǎn)期奶牛血清中GSH-Px、CAT含量有升高的趨勢,但是差異不顯著。試驗三:結(jié)果顯示:(1)催乳保健散可以有效提高產(chǎn)奶初期奶牛的產(chǎn)奶量及4%乳脂校正乳;(2)極顯著提高奶牛初乳中乳糖率,顯著提高乳脂率,對初乳中乳蛋白率有一定影響,但是差異不顯著;(3)極顯著提高產(chǎn)后14d、28d牛奶中乳糖率;顯著提高產(chǎn)后14d、28d、42d、56d、70d乳脂率,分別為11.11%、10.51%、5.08%、5.45%、4.42%;極顯著提高70d、84d時試驗組奶牛常乳中乳蛋白率。試驗四:結(jié)果顯示:催乳保健散可以極顯著提高圍產(chǎn)期奶牛的Ca、P表觀消化率(P0.01);顯著提高圍產(chǎn)期奶牛的DMI及其對DM與NDF的表觀消化率(P0.05)。結(jié)論:綜合分析可知:免疫增強(qiáng)劑可以有效提高圍產(chǎn)期奶牛的免疫機(jī)能,提高圍產(chǎn)期奶牛的抗氧化應(yīng)激能力;催乳保健散可以顯著提高圍產(chǎn)期奶牛的產(chǎn)奶量及4%乳脂校正乳,可以有效提高牛奶中各乳成分含量;催乳保健散可以促進(jìn)圍產(chǎn)期奶牛對各營養(yǎng)物質(zhì)的表觀消化率,提高干物質(zhì)采食量,有效改善圍產(chǎn)期奶牛的能量負(fù)平衡狀態(tài)。
[Abstract]:Objective: to study the effect of immune enhancer and prolactin powder on the immune function and antioxidant ability of perinatal cows by preventing negative balance of energy, immune stress and oxidative stress in perinatal cows. The effects of prolactin powder on milk yield and apparent digestibility of nutrients in perinatal dairy cows were discussed, which laid a theoretical and experimental foundation for further study on the mechanism of immune booster and prolactin health powder on perinatal cows. Methods: in experiment 1, the serum cytokines (IL-2IL-6TNF- 偽) and immunoglobulin Ig (Ig / Ig) were detected by adding immunoenhancer to the diet of the perinatal cows and collecting the blood of the caudal vein of the cows, using the kit to detect the contents of the cytokines (TNF- 偽) and the immunoglobulin (Ig). Experiment 2: adding immunoenhancer to the diet of perinatal cows and collecting the blood of caudal vein of dairy cattle, using the test kit to detect the content of GSH-PxX SODCATCATDAs in serum. Experiment 3: adding prolactin and health powder to the diet of perinatal dairy cows, recording the milk yield in 3 months postpartum, detecting the content of each component (lactose, milk protein, milk fat) in the milk sample, and calculating the corrected milk quantity of 4% milk fat. Experiment 4: adding prolactin and health powder to the diet of the perinatal dairy cow, collecting the ration and fresh feces of the cow regularly, and detecting the contents of dry matter, crude protein, neutral detergent fiber, calcium and phosphorus in the diet. The dry matter intake and apparent digestibility of each nutrient were calculated. Results: experiment 1: the results showed that the immune-enhancer could significantly increase the content of Ig / Ig / Ig in perinatal cow serum (P0.01P0.01P0. 01). However, the effect of immunoenhancer on the content of TNF- 偽 and IL-2 in serum was not stable. Experiment 2: the results showed that the content of SOD in serum of perinatal cows was significantly increased by immunoenhancer, and the content of MDA in serum of perinatal cows was decreased significantly. The content of GSH-PxPxcat in serum of perinatal cows increased, but the difference was not significant. Experiment 3: the results showed that the prolactin powder could effectively increase the milk yield and the 4% milk fat correction milk of the early stage cow.) the lactose rate and the milk fat rate in the bovine colostrum were significantly increased, and the milk protein rate was affected to some extent. But the difference was not significant (P < 0.05). The lactose rate in milk was significantly increased by 14 days postpartum to 28 days, and the milk fat rate at 14 days after delivery by 28 days to 42 days and 56 days to 70 days, respectively, which was 11.111.10.51and 5.085.450.The milk protein rate of the test group was significantly increased at the day of 70d 84d, and the milk protein rate of the test group was significantly higher than that of the control group on the 14th day postpartum and the milk fat rate of the control group was significantly higher than that of the control group on the 14th day after delivery. Experiment 4: the results showed that the apparent digestibility of Ca-P and the apparent digestibility of DM and NDF in perinatal cows were significantly increased by prolactin and health powder, respectively, and the apparent digestibility of NDF and DM were significantly increased in perinatal cows. Conclusion: the comprehensive analysis shows that the immune enhancer can effectively improve the immune function and the antioxidant stress ability of the perinatal cows, and the prolactin health powder can significantly increase the milk yield and 4% milk fat correction milk of the perinatal cows. Prolactin powder can promote the apparent digestibility of the nutrients, increase the intake of dry matter and improve the negative energy balance of the cows during the perinatal period.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S853.7

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