本文選題：犬細(xì)小病毒 + 犬瘟熱病毒��； 參考：《吉林農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：隨著人們生活水平的提高,犬、貓等伴侶動物已逐漸成為人們生活中重要的組成部分,犬貓疾病也隨之成為威脅寵物健康的主要因素。犬細(xì)小與犬瘟熱是寵物犬病毒病中傳染性強、發(fā)病率高、導(dǎo)致患病犬死亡率最高的急性傳染病,也是寵物臨床中最常見的犬科疾病,在世界范圍內(nèi)大規(guī)模流行,對寵物犬的健康造成了嚴(yán)重的威脅。本試驗對吉林省長春地區(qū)犬瘟熱病毒(Canine distemper virus,CDV)與犬細(xì)小病毒(Canine parvovirus,CPV)的流行病學(xué)及遺傳進化差異進行了調(diào)查與分析,以期為CPV與CDV病原學(xué)調(diào)查的進一步研究和防控策略的制定提供數(shù)據(jù)支持。本試驗采用膠體金快速檢測試劑盒對2015年12月~2017年1月吉林省長春地區(qū)9個不同區(qū)/縣寵物醫(yī)院就診的539只犬進行了CPV、CDV的病原學(xué)調(diào)查。結(jié)果表明:CPV陽性率為28.57%,CDV陽性率為17.25%,CPV+CDV陽性率3.15%;CPV在3月份發(fā)病率最高,為38.29%,在7、8、9月份的發(fā)病率最低,分別為16.13%、16.27%、16.67%,CDV在3、4、11月份的發(fā)病率最高,分別為21.28%、24.36%、22.22%,8月份發(fā)病率最低,為9.30%;2~4月齡犬CPV發(fā)病率最高,為38.17%,CDV發(fā)病率最高的年齡段為0~2月齡(23.81%);公犬CPV陽性率為29.70%,CDV陽性率為17.82%,母犬CPV陽性率為27.12%,CDV陽性率為16.53%,差異不顯著;小型犬CPV及CDV發(fā)病率最高,分別為32.99%、19.79%,大型犬CPV及CDV發(fā)病率最低,分別為22.61%、14.78%;未免疫犬CPV、CDV發(fā)病率最高,未完成完全免疫的犬發(fā)病率次之,發(fā)病率最低的為完全免疫的犬。經(jīng)分析證明,CPV、CDV發(fā)病率與地區(qū)、性別關(guān)系不顯著,與月份、年齡、品種、血統(tǒng)、免疫情況關(guān)系顯著�；贑PV VP2基因、CDV H基因分析長春地區(qū)CPV、CDV基因型分布情況。結(jié)果表明,隨機挑選的36份CPV陽性樣品中,CPV-2基因型占5.56%,CPV-2a基因型占61.11%,CPV-2b基因型占30.56%,CPV-2c基因型為占2.78%;隨機挑選的12份CDV陽性樣品中,CDV AsiaⅠ基因型占91.67%,CDV America基因型占8.33%。本研究表明長春地區(qū)寵物醫(yī)院就診犬CPV發(fā)病率高于CDV,其中0~4月齡犬CPV、CDV發(fā)病率高,主要發(fā)生于3、4、11月份,易發(fā)群體主要表現(xiàn)為中小型犬、純種犬、未免疫犬。本次調(diào)查中長春地區(qū)CPV流行毒株主要為CPV-2a基因型,其次為CPV-2b基因型;CDV流行毒株主要為AsiaⅠ基因型。
[Abstract]:With the improvement of people's living standards, dogs, cats and other companion animals have gradually become an important part of people's lives, and dog and cat diseases have become the main factors threatening the health of pets. Canine parvovirus and canine distemper is an acute infectious disease with high incidence and the highest mortality in dogs. It is also the most common canine disease in pet clinic. It poses a serious threat to the health of the pet dog. The epidemiological and genetic differences of canine distemper virus (canine distemper virus) and canine parvovirus (canine parvovirus) in Changchun, Jilin Province, were investigated and analyzed. In order to provide data support for the further study of CPV and CDV etiology investigation and the formulation of prevention and control strategy. From December 2015 to January 2017, 539 dogs in 9 different areas / counties of Changchun, Jilin Province, were investigated for CPV CDV by colloidal gold rapid detection kit. The results showed that the positive rate of CDV was 17.25% in March, 38.29 in March, and the lowest in September, 16.130.16.270.The incidence of CDV was the highest in November, 21.2824.36 and 22.2222 in August, respectively. The highest incidence of CPV was found in dogs of 4 months old (38.17). The positive rates of CPV in male dogs were 29.70% and 17.82% respectively, and the positive rates of CPV in female dogs were 16.533.The incidence rates of CPV and CDV in small dogs were the highest, and the positive rates of CPV were 17.82% in male dogs and 17.82% in female dogs, and the incidence rates of CPV and CDV in small dogs were the highest. The incidence of CPV and CDV in large dogs was the lowest (22.61 and 14.78, respectively), the incidence of CPV in unimmunized dogs was the highest, and that in dogs without complete immunization was the second, and the lowest in dogs with complete immunization. The analysis showed that the incidence of CPV CDV was not significantly related to gender, but to month, age, variety, lineage and immune status. The distribution of CDV genotypes in Changchun area was analyzed based on CPV VP2 gene and CDV H gene. The results showed that the CPV-2 genotype was 5.56% and the CPV-2a genotype was 61.11%, and the CPV-2b genotype was 30.565.56% and the CPV-2c genotype was 2.78% in the 36 randomly selected CDV positive samples, and in the 12 randomly selected CDV positive samples, 91.67 Asia 鈪，

本文編號：1881528