本文選題：肉仔雞 + 換料應(yīng)激��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：為探討換料應(yīng)激和自然霉變玉米對(duì)肉仔雞食欲的影響,本研究以活體入手,分別用顆粒飼料直接替換粉料或自然霉變玉米配制飼料,觀察家禽的采食行為,同時(shí)分析下丘腦和腸道內(nèi)食欲相關(guān)基因表達(dá)情況,為深入肉仔雞的食欲調(diào)控機(jī)制提供一定的理論依據(jù)。首先,本研究通過(guò)在21日齡用顆粒料直接替換粉料,建立肉仔雞換料應(yīng)激模型,考察換料應(yīng)激對(duì)肉仔雞采食量的影響。試驗(yàn)一、選擇體重相近的1日齡從肉仔雞80只,分成2個(gè)處理,每個(gè)處理4個(gè)重復(fù),每個(gè)重復(fù)10只雞,所有肉仔雞飼喂粉料到20日齡,21日齡早上8：00一個(gè)組繼續(xù)飼喂粉料(對(duì)照組),另一個(gè)組用顆粒料代替粉料(換料料組),換料后每隔1h統(tǒng)計(jì)一次采食量。試驗(yàn)二、選擇體重相近的1日齡AA肉仔雞200只,分成2個(gè)處理,每個(gè)處理4個(gè)重復(fù),每個(gè)重復(fù)25只雞,所有肉仔雞飼喂粉料到20日齡,21日齡早上8：00一個(gè)組繼續(xù)飼喂粉料(對(duì)照組),另一個(gè)組用顆粒料代替粉料(換料組),分別在6h；12h；24h每個(gè)處理隨機(jī)取8只雞取腺胃,十二指腸,空腸,回腸。試驗(yàn)三、選擇體重相近的1日齡AA肉仔雞400只,分為4個(gè)處理,每個(gè)處理4個(gè)重復(fù),每個(gè)重復(fù)25只雞,試驗(yàn)期0-22日齡,17日齡隨機(jī)選擇一個(gè)組開(kāi)始飲用添加2%谷氨酰胺或天冬氨酸的水,飼喂粉料到20日齡,21日齡早上8：00兩個(gè)組均換料,換料后每隔兩個(gè)小時(shí)統(tǒng)計(jì)一次采食量。試驗(yàn)四、選擇體重相近的1日齡AA肉仔雞480只,隨機(jī)分成三個(gè)組,飼喂粉料到17日齡,18日齡時(shí)隨機(jī)選取其中一組飼喂粉料時(shí)表面覆蓋30%顆粒料(預(yù)處理組),其余兩組繼續(xù)飼喂粉料至20日齡。.21日齡開(kāi)始正式試驗(yàn),另外兩個(gè)飼喂粉料組一個(gè)組繼續(xù)飼喂粉料(對(duì)照組),另一個(gè)組用顆粒料代替粉料(換料組),換料后每隔2h統(tǒng)計(jì)一次采食量,分別在6h；24h每個(gè)處理隨機(jī)取8只雞采血,并采集下丘腦,腺胃,十二指腸,空腸,回腸。試驗(yàn)主要結(jié)果顯示,換料應(yīng)激降低肉仔雞采食量的調(diào)控機(jī)制是提高腸道中g(shù)hrelin水平,而提前表面覆蓋顆粒料可以通過(guò)調(diào)節(jié)下丘腦的食欲因子和降低腸道ghrelin水平來(lái)緩解換料應(yīng)激對(duì)肉雞采食量的影響。其次,本研究通過(guò)用100%自然霉變玉米配制飼料代替正常飼料,考察自然霉變玉米對(duì)家禽采食量的影響。試驗(yàn)一、選取240只體重相近的1日齡海蘭褐蛋公雛,分成兩個(gè)組,對(duì)照組和試驗(yàn)組(霉變玉米組),每個(gè)組6個(gè)重復(fù),每個(gè)重復(fù)20只雞,試驗(yàn)共14天,每天早上8：00統(tǒng)計(jì)采食量。生長(zhǎng)試驗(yàn)結(jié)束后,每個(gè)重復(fù)選擇2只雞,禁食12h后取血,采集血清,十二指腸,空腸,回腸。試驗(yàn)二、選取240只體重相近的1日齡AA肉仔雞,分成兩個(gè)組,對(duì)照組和試驗(yàn)組(霉變玉米組),每個(gè)組6個(gè)重復(fù),每個(gè)重復(fù)20只雞,試驗(yàn)共14天,每天早上8：00統(tǒng)計(jì)采食量。生長(zhǎng)試驗(yàn)結(jié)束后,每個(gè)重復(fù)選擇2只雞,禁食12h后取血,采集血清、下丘腦、肝臟、十二指腸、空腸、回腸。試驗(yàn)主要結(jié)果顯示,短期飼喂自然霉變玉米對(duì)蛋公雛攝食無(wú)不良影響,但是影響肉仔雞腸道發(fā)育,從而對(duì)肉仔雞發(fā)育有不良影響。
[Abstract]:In order to investigate the effect of feeding stress and natural mildew corn on the appetite of broilers, this study, starting with the living body, used particle feed directly to replace powder or natural mouldy corn to prepare feed, observed the feeding behavior of poultry, and analyzed the expression of appetite related genes in the hypothalamus and intestines, in order to deepen the appetite regulation mechanism of broilers. To provide a certain theoretical basis. First, by replacing the powder directly with particles at 21 days of age, the stress model of broilers was established, and the effect of stress on the feed intake of broilers was investigated. The broilers were fed at 20 days of age, and at 8:00 in the morning of 21 days, one group continued to feed the powder (control group). The other group used particle material instead of powder material (feeding material group). After the meal, the feed intake was counted every 1H. The test two, the 1 day old AA broilers of similar weight were selected and 200 were divided into 2 treatments, each treated 4 repetitions, each repeated 25 chickens, All broilers were fed powder for 20 days of age, and at 8:00 in the morning of 21 days, one group continued to feed powder (control group), the other group used granule material instead of powder (feeding group), in 6h; 12h; 24h each treatment randomly took 8 chickens for glandular stomach, duodenum, jejunum and ileum. Three of the 1 day old AA broilers with similar weight were selected and 4 were divided into 4. Each treatment was treated with 4 repetitions, each repeated 25 chickens, the test period was 0-22 days old and 17 days of age randomly selected a group to start drinking with 2% glutamine or aspartic acid water, feeding powder to 20 days of age and two groups at 8:00 in the morning of 21 days. After feeding, the feed intake was counted every two hours. Four of the experiment was selected, and the body weight was close to the similar weight. 480 AA broilers of 1 days of age were randomly divided into three groups, feeding powder to 17 days of age. At 18 days of age, one group was randomly selected to cover 30% particles (pre treatment group) when feeding powder. The other two groups continued to be fed to the age of 20 days of age to start the formal test, and the other two fed powder groups continued to feed powder (control group). The other group used particle material instead of powder (feeding group). After changing the feed, the feed intake was counted every 2h, and 8 chickens were collected at random in 6h and 24h, and the hypothalamus, glands, stomach, duodenum, jejunum and ileum were collected. The main results showed that the regulation mechanism of reducing feed intake by feeding stress was to improve the level of ghrelin in the intestinal tract. The effect of advance surface covering particles on the appetite factor of the hypothalamus and the decrease of the ghrelin level of the intestinal tract could be used to alleviate the effect of the feeding stress on the feed intake. Secondly, the effect of natural mildew corn on the feed intake of domestic fowl was investigated by using 100% natural mouldy corn feed instead of normal feed. The 1 day old brown egg male chicks of 1 days of age were divided into two groups, the control group and the test group (mildew corn group), each group was repeated 6 repetitions, each repeated 20 chickens. The test was 14 days. At the end of the test, 2 chickens were selected repeatedly, after fasting 12h, the blood was taken, the duodenum, jejunum and ileum were collected. Test two, 240 AA broilers of 1 days of age with similar weight were selected, divided into two groups, control group and test group (mildew corn group), each group was 6 repeats, each repeated 20 chickens, the test was 14 days, at 8:00 every morning to count the food intake. After the growth test, each repeated selection of 2 chickens, after fasting 12h, collected blood, collected serum, hypothalamus, liver, ten Two finger intestines, jejunum and ileum. The main results showed that the short term feeding of natural mouldy corn had no adverse effects on the feeding of egg male chicks, but it affected the development of enteron of meat chicks and had adverse effects on the development of broilers.

【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李W，

本文編號(hào)：1874820