發(fā)布時(shí)間：2018-05-08 14:56

  本文選題：肉雞 + 腎臟��； 參考：《華中農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：多巴胺是機(jī)體內(nèi)最主要的兒茶酚胺類遞質(zhì)之一,隨著人們對(duì)多巴胺作用機(jī)理的深入研究,發(fā)現(xiàn)許多疾病如抽動(dòng)穢語綜合征(Tourette綜合征),精神分裂癥,帕金森病等都與多巴胺神經(jīng)遞質(zhì)傳導(dǎo)有密切聯(lián)系。低劑量多巴胺具有利尿利鈉的作用,在人醫(yī)臨床上應(yīng)用極為廣泛,但在肉雞此方面的研究尚未見報(bào)道。腎臟具有維持機(jī)體酸堿平衡、水鹽代謝以及內(nèi)環(huán)境穩(wěn)態(tài)等功能,其主要是通過鈉鉀氯共轉(zhuǎn)運(yùn)體(NKCC2)、鈉氯共轉(zhuǎn)運(yùn)體(NCC)和水通道蛋白2(AQP2)等對(duì)水鹽代謝的調(diào)控來實(shí)現(xiàn)的。研究表明,低劑量多巴胺具有利鈉利尿作用,對(duì)腎小管鈉水的重吸收具有較強(qiáng)的抑制作用。為研究低劑量多巴胺對(duì)肉雞腎臟鈉鉀氯共轉(zhuǎn)運(yùn)體(NKCC2)、鈉氯共轉(zhuǎn)運(yùn)體(NCC)和水通道蛋白2(AQP2)表達(dá)的影響,本試驗(yàn)按常規(guī)條件飼養(yǎng)AA肉雞40羽,25日齡時(shí)隨機(jī)分為對(duì)照組(C組=20)和試驗(yàn)組(D組=20),試驗(yàn)組肉雞經(jīng)靜脈注射給予低劑量左旋多巴胺(5μg/kg/min),對(duì)照組肉雞按相同方法給予等體積0.9%生理鹽水。并采用組織形態(tài)學(xué)觀察、血清生化檢測(cè)、蛋白質(zhì)免疫印跡試驗(yàn)和RT-q PCR等方法,檢測(cè)肉雞腎組織形態(tài)學(xué)和各種生化指標(biāo)的變化,及鈉鉀氯共轉(zhuǎn)運(yùn)體(NKCC2)、鈉氯共轉(zhuǎn)運(yùn)體(NCC)和水通道蛋白2(AQP2)蛋白水平和m RNA水平的變化。結(jié)果顯示:與對(duì)照組肉雞相比,試驗(yàn)組在給以低劑量左旋多巴胺后,尿素氮、肌酐(Cre)及動(dòng)脈壓(m PAP)水平均未發(fā)生明顯改變(P0.05);低劑量左旋多巴胺持續(xù)給藥后,肉雞腎小管上皮細(xì)胞胞膜NKCC2、NCC、AQP2蛋白水平顯著降低,細(xì)胞質(zhì)NKCC2、NCC、AQP2蛋白顯著升高(P0.05);然而NKCC2、NCC、AQP2 m RNA水平未發(fā)生明顯變化(P0.05)。本試驗(yàn)從腎臟水鈉代謝途徑中的關(guān)鍵水鈉通道蛋白的表達(dá)變化著手,探討了低劑量多巴胺對(duì)肉雞水鈉通道蛋白調(diào)節(jié)的機(jī)制。實(shí)驗(yàn)結(jié)果表明,在不影響腎小球?yàn)V過率及血壓前提下,低劑量多巴胺能夠引起肉雞機(jī)體持續(xù)的利鈉利尿作用,其機(jī)制與NKCC2、NCC、AQP2蛋白的穿梭作用有關(guān)。
[Abstract]:Dopamine is one of the most important catecholamine transmitters in the body. With the further study of the mechanism of dopamine, many diseases such as Tourette syndrome and schizophrenia have been found. Parkinson's disease is closely related to dopamine neurotransmitter conduction. Low dose dopamine has diuretic natriuretic effect and has been widely used in human medicine, but it has not been reported in broiler. The kidney has the functions of maintaining the balance of acid and base, water and salt metabolism, and the homeostasis of the internal environment, which is mainly realized by the regulation of water and salt metabolism by the sodium, potassium and chlorine co-transporters NKCC2, sodium chloride co-transporter NCC2, and aquaporin 2aquaporp. The results showed that low dose dopamine had diuretic effect and inhibited the reabsorption of renal tubular sodium water. To investigate the effects of low dose dopamine on the expression of NKCC2N, NCC2 and aquaporin 2AQP2in the kidney of broilers. In this study, 40 AA broilers were randomly divided into control group (group C) and control group (group D) at 25 days of age under conventional conditions. The experimental group was given low dose of L-dopamine 5 渭 g / kg / min by intravenous injection, while the control group was given the same method. The volume of saline was 0.9%. Histomorphologic observation, serum biochemical test, Western blot test and RT-q PCR were used to detect the changes of renal histomorphology and biochemical indexes in broilers. The changes of protein levels and m RNA levels of sodium potassium chloride co-transporter NKCC2, sodium chloride co-transporter NCC2 and aquaporin-2aquaporin (AQP2). The results showed that compared with the control group, the levels of urea nitrogen, creatinine (creatinine) and arterial pressure (MPP) in the experimental group were not significantly changed after the low dose of L-dopamine was given, and the low dose of levodopamine was given continuously. The protein level of NKCC2 / NCC-AQP2 in renal tubular epithelial cells of broilers was significantly decreased, while the protein level of NKCC2 / NCC-AQP2 was significantly increased in the cytoplasm of NCC2, but the level of NKCC2 / NCCP AQP2 m was not changed significantly (P 0.05). In this study, we studied the mechanism of low dose dopamine on the regulation of sodium channel protein in broilers by analyzing the changes of the expression of sodium channel protein in renal water sodium metabolism pathway. The results showed that, without affecting glomerular filtration rate and blood pressure, low dose dopamine could induce continuous diuretic natriuretic effect in broilers, and its mechanism was related to the shuttle effect of NKCC2NCC-AQP2 protein.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S859.7

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