本文選題：豬圓環(huán)病毒2型 + 抗體檢測�。� 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：豬圓環(huán)病毒2型(PCV2)在豬群中感染率較高,除了本身可以引起豬發(fā)病外可還會(huì)造成免疫系統(tǒng)損害,引起多種疾病的發(fā)生。疫苗免疫接種可以減少PCV2對免疫豬的危害程度、組織中病毒的載量,也為豬場進(jìn)行PCV2的凈化提供了基礎(chǔ)條件。疫苗免疫后的抗體水平是評價(jià)其效果的主要指標(biāo),對免疫豬體內(nèi)PCV2病毒的檢測是評價(jià)疫苗效果的最直接指標(biāo)。在專業(yè)實(shí)習(xí)期間,主要從事PCV2疫苗免疫效果的評價(jià),先后采集了7個(gè)長期堅(jiān)持進(jìn)行PCV2的豬場豬血清,2個(gè)未進(jìn)行PCV2疫苗免疫豬場的血清樣品,對其血清抗體和病毒進(jìn)行檢測,以期從抗體水平和病毒清除方面說明疫苗免疫在豬圓環(huán)病毒病防控中的作用。在分析疫苗免疫和病毒感染關(guān)系的基礎(chǔ)上,本研究又將檢測為PCV2核酸陽性的樣品中克隆了其rep和cap基因,進(jìn)行序遺傳變異分析,以期從核酸水平上分析PCV2感染的原因,也為疫苗研制提供基礎(chǔ)資料。本研究獲得以下結(jié)果:(1)從來自7個(gè)PCV2疫苗免疫豬場的178份血清中抗體陽性率65.17%(116/178),最高抗體陽性率達(dá)到90.20%,最低僅有26.67%;2個(gè)非PCV2疫苗免疫豬場的84份樣品中抗體陽性率15.48%(13/84)。(2)262份血清樣品中6份PCV2核酸陽性,占2.29%(6/262)。在7個(gè)疫苗免疫場中有2個(gè)場的血清中檢出PCV2核酸,陽性率分別為3.33%(1/30)和9.09%(1/11)。非免疫豬場1的陽性率為2.86%(1/35),豬場2為6.12%(3/49)。(3)PCV2核酸陽性的樣品其特異性抗體均為陰性。(4)6個(gè)分離株的rep基因序列的同源性較高,為97.3%~99.8%;GS1、3、4的同源性較高,GS2、5、6同源性較高,GS1、3、4與GS2、5、6的同源性較低。(5)6個(gè)分離株之間以及與國內(nèi)外分離株之間均存在一些基因差異,分離株的cap基因之間同源性最高為98.1%,最低為87.7%;在進(jìn)化樹上同屬于一個(gè)大的分支,和國內(nèi)分離的PCV2d基因亞型的毒株存在較高的同源性。
[Abstract]:The infection rate of porcine circovirus type 2 (PCV2) is higher in pigs, which can cause immune system damage and cause many diseases. Vaccination can reduce the harm of PCV2 to immunized pigs and the viral load in the tissues. It also provides the basic conditions for the purification of PCV2 in pig farms. The level of antibody after immunization is the main index to evaluate the effect of the vaccine, and the detection of PCV2 virus in immunized pigs is the most direct index to evaluate the effect of vaccine. During the period of professional practice, he was mainly engaged in the evaluation of the immune effect of PCV2 vaccine. Seven pig serum samples which persisted in PCV2 for a long period of time, and two serum samples from pig farm without PCV2 vaccine immunization were collected, and their serum antibodies and viruses were detected. In order to explain the role of vaccine immunization in prevention and control of porcine circovirus disease from antibody level and virus clearance. Based on the analysis of the relationship between vaccine immunization and viral infection, the rep and cap genes were cloned from the samples positive for PCV2 nucleic acid, and the sequence genetic variation analysis was carried out in order to analyze the causes of PCV2 infection at nucleic acid level. It also provides basic information for vaccine development. The results of this study were as follows: 1) the antibody positive rate of 178 sera from seven PCV2 vaccine vaccination farms was 65.17 / 116 / 178. The highest antibody positive rate was 90.20 and the lowest was 26.67. The antibody positive rate of 84 samples from two non-PCV2 vaccine vaccination farms was 15.48% 13 / 84 .2262%. Six of the serum samples were PCV2 nucleic acid positive. 2.29 / 262a. PCV2 nucleic acid was detected in 2 of the 7 vaccine immunization fields, the positive rates were 3.33 / 30) and 9.09% / 11 ~ (-1 / 11), respectively.The positive rate of PCV2 nucleic acid was 3.33% and 9.09% / 11%, respectively. The positive rate of non-immunized pig farm 1 was 2.86 / 35. The rep gene sequence of the six isolates were highly homologous to each other in the samples with 6.12%(3/49).(3)PCV2 nucleic acid positive and the specific antibodies were all negative. There were some genetic differences among the six isolates, and between the six isolates and the domestic and foreign isolates. The highest homology of cap gene was 98.1 and the lowest was 87.7. The cap gene belonged to a large branch in the evolutionary tree and had high homology with the domestic isolates of PCV2d gene subtype.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.28

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