本文選題：多糖-硒化多糖復(fù)方 + 淋巴細胞增殖��； 參考：《南京農(nóng)業(yè)大學學報》2017年03期

【摘要】：[目的]驗證多糖-硒化多糖復(fù)方的增強免疫活性,篩選出活性最強的復(fù)方,為研制新型免疫增強劑提供試驗依據(jù)。[方法]在前期研究篩選出增強免疫活性較強的當歸多糖(CAP)、黨參多糖(CPP)、大蒜多糖(GP)、硒化當歸多糖(sCAP)、硒化黨參多糖(sCPP)和硒化大蒜多糖(s GP)的基礎(chǔ)上,以其為組分藥,按照多糖與硒化多糖質(zhì)量比9∶1的比例組成CAP-sCPP、CAP-s GP、CPP-sCAP、CPP-s GP、GP-sCAP和GP-sCPP共6個復(fù)方,比較它們的體內(nèi)/外增強免疫活性能力。體外試驗,用MTT法測定6個復(fù)方對雞外周血淋巴細胞增殖的影響;體內(nèi)試驗,進一步比較了篩選出的3個復(fù)方對新城疫苗免疫雛雞淋巴細胞增殖,血清抗體效價,IL-2、IL-6和IFN-γ含量的影響。[結(jié)果]體外試驗結(jié)果顯示:單獨或與植物血凝素(PHA)共同刺激時,6個復(fù)方均有不同程度的增強免疫活性,CAP-sCPP、GP-sCPP和CPP-sCAP的活性較強;體內(nèi)試驗結(jié)果顯示:在免疫后7、14、21和28 d,GP-sCPP高劑量促進T淋巴細胞增殖的作用最強,血清抗體效價和IL-2、IL-6及IFN-γ含量均為最高,且均顯著高于免疫對照組。[結(jié)論]GP-sCPP的增強免疫活性最強,可以考慮作為新型免疫增強劑的候選方。
[Abstract]:[objective] to verify the enhanced immune activity of polysaccharides-selenide polysaccharide compound, and to screen out the most active compound, and to provide the experimental basis for the development of new immune enhancer. [methods] on the basis of screening out the polysaccharides of Angelica sinensis, Codonopsis pilosula, polysaccharide of garlic, polysaccharide of Angelica sinensis, polysaccharide of decanum selenidum and polysaccharide of Se-treated garlic, which were selected in the previous study, the polysaccharides of Angelica sinensis and Allium selenidum were selected as components. According to the ratio of polysaccharides to polysaccharides (9:1), CAP-sCPPU / CAP-s GPP / CPP-s GPGP-sCAP and GP-sCPP were composed of six compounds to compare their immunological activity in vivo and in vitro. In vitro, the effects of six compounds on the proliferation of chicken peripheral blood lymphocytes were determined by MTT method, and the proliferation of lymphocytes of chickens immunized with Xincheng vaccine was further compared in vivo. The effect of serum antibody titer on IL-2, IL-6 and IFN-緯 levels. [results] the results of in vitro test showed that all of the six compounds had enhanced immunological activity in different degrees when stimulated alone or with phytohemagglutinin (PHA). The activities of GP-sCPP and CPP-sCAP were stronger than those of CAP-sCPPU alone or in combination with phytohemagglutinin (PHA). The results of in vivo test showed that GP-sCPP had the strongest effect on the proliferation of T lymphocytes on the 7th and 28th days after immunization, the titer of serum antibody and the contents of IL-6 and IFN- 緯 were the highest, and they were significantly higher than those of the control group. [conclusion] GP-sCPP has the strongest enhanced immune activity and can be considered as a candidate for new immunoenhancers.
【作者單位】： 南京農(nóng)業(yè)大學中獸醫(yī)學研究室;
【基金】：國家自然科學基金項目(31272596) 國家公益性行業(yè)(農(nóng)業(yè))科研專項(201403051)
【分類號】：S831.5

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