本文選題：玉米赤霉烯酮 + 脫氧雪腐鐮刀菌烯醇��； 參考：《中國畜牧獸醫(yī)》2017年05期

【摘要】：為研究鐮刀菌毒素玉米赤霉烯酮(ZEA)、脫氧雪腐鐮刀菌烯醇(DON)及其聯(lián)合作用對動物免疫功能的影響,試驗以CTLL-2細胞(細胞毒性T淋巴細胞株)為材料,用不同濃度的ZEA(0、5、10、20μg/mL)、DON(0、0.5、1、2μg/mL)及聯(lián)合(空白組、5μg/mL ZEA、0.5μg/mL DON、5μg/mL ZEA+0.5μg/mL DON)處理CTLL-2細胞48h,采用ELISA法檢測了細胞內(nèi)及培養(yǎng)上清液中顆粒酶B(GZMB)、穿孔素(PFP)、干擾素γ(IFN-γ)和腫瘤壞死因子α(TNF-α)等細胞因子的含量。結(jié)果顯示,ZEA、DON能夠降低CTLL-2細胞胞內(nèi)及培養(yǎng)上清液中PFP、GZMB、IFN-γ的濃度,增加TNF-α濃度,染毒組與對照組相比均有顯著或極顯著差異(P0.05;P0.01),且均呈劑量效應(yīng)關(guān)系;ZEA、DON聯(lián)合染毒表現(xiàn)為加性效應(yīng)。結(jié)果表明,ZEA、DON及其聯(lián)合作用可通過影響免疫細胞因子的分泌,降低免疫細胞殺傷活力,間接影響機體體液免疫和細胞免疫的負調(diào)節(jié),從而導(dǎo)致動物機體免疫機能下降。
[Abstract]:In order to study the effect of Fusoxin ZEAA, deoxynivalenoldol (DON) and its combined action on the immune function of animals, CTLL-2 cells (cytotoxic T lymphocyte strain) were used as materials. The cell line CTLL-2 cells were treated with different concentrations of ZEAA (0.5 渭 g/mL, 0.5 渭 g/mL ZEA, 0.5 渭 g/mL DON-1) and combined (5 渭 g/mL, 0.5 渭 g/mL DONs). The levels of cytokines such as granulase Bg/mL GZMBN, perforin PFPN, interferon 緯 IFN- 緯) and tumor necrosis factor- 偽 (TNF- 偽) were determined by ELISA assay. The results showed that ZEADON could decrease the concentration of IFN- 緯 in CTLL-2 cells and culture supernatant, and increase the concentration of TNF- 偽. Compared with the control group, there were significant or extremely significant differences between the two groups. The results showed that ZEADON and its combined action could decrease the killing activity of immune cells by affecting the secretion of immune cytokines and indirectly affect the negative regulation of humoral and cellular immunity, resulting in the decrease of immune function in animals.
【作者單位】： 揚州大學(xué)獸醫(yī)學(xué)院;江蘇省動物重要疫病與人獸共患病防控協(xié)同創(chuàng)新中心;
【基金】：江蘇省普通高校自然科學(xué)基金(08KJD230002) 江蘇高校優(yōu)勢學(xué)科建設(shè)工程資助項目(PAPD)
【分類號】：S859.87

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