本文選題：山羊 + 生長性狀�。� 參考：《貴州大學(xué)》2015年碩士論文

【摘要】：本研究以貴州省地方優(yōu)良山羊品種——貴州白山羊、貴州黑山羊和黔北麻羊3種山羊作為研究對(duì)象,試驗(yàn)山羊共計(jì)337只,利用DNA混合池、PCR-SSCP、DNA直接測序等方法研究STAT5A和STAT5B基因單核苷酸多態(tài)性,并分別研究了不同山羊品種群體遺傳結(jié)構(gòu)、遺傳多樣性與生長性狀的關(guān)聯(lián)分析,以期得到普遍適用于能顯著提高山羊各品種個(gè)體生長性狀的分子遺傳輔助標(biāo)記。同時(shí),本研究還利用q PCR技術(shù)分析了貴州白山羊STAT5A和STAT5B基因在9個(gè)不同組織中的表達(dá)。研究結(jié)果如下:1在三種山羊STAT5A和STAT5B基因中分別篩查到3個(gè)SNPs2 STAT5A基因C11447T位點(diǎn)對(duì)貴州白山羊體高有顯著影響,G14264A位點(diǎn)對(duì)貴州黑山羊體重、體高和胸圍有顯著影響,G14320A位點(diǎn)對(duì)貴州白山羊體斜長、管圍和胸圍及貴州黑山羊體重和胸圍有顯著影響(P0.05)3 STAT5B基因T16159C位點(diǎn)對(duì)貴州白山羊體重、體斜長、管圍和胸圍及黔北麻羊體重、體高、管圍和胸圍有顯著影響,C20844T位點(diǎn)對(duì)貴州白山羊體重、體斜長、管圍和胸圍及貴州黑山羊胸圍有顯著影響,C21455T位點(diǎn)對(duì)貴州白山羊5個(gè)生長性狀,貴州黑山羊體重和胸圍及黔北麻羊體重、體高和體斜長有顯著影響(P0.05)4 STAT5A和STAT5B基因在貴州白山羊不同年齡階段表達(dá)量(1)STAT5A周歲羊:肝垂體心下丘腦肺脾小腦大腦腎;(2)STAT5A成年羊:心肺大腦垂體下丘腦小腦脾肝腎;(3)STAT5B周歲羊:肝垂體心脾小腦肺大腦腎下丘腦;(4)STAT5B成年羊:心肺大腦肝垂體腎脾小腦下丘腦。5 STAT5A和STAT5B基因在貴州白山羊周歲羊肝臟和垂體中表達(dá)最高,在貴州白山羊成年羊心臟、肺和大腦表達(dá)最高,暗示這兩個(gè)基因是調(diào)控山羊早期生長的重要基因,且隨著山羊個(gè)體的發(fā)育成熟,其主要作用和作用部位發(fā)生改變,同時(shí)這兩個(gè)基因具有高度的協(xié)調(diào)性與一致性。
[Abstract]:In this study, three kinds of goats, Guizhou white goat, Guizhou black goat and Qianbei Ma goat, were used as the research objects. A total of 337 goats were tested. Single nucleotide polymorphisms (SNP) of STAT5A and STAT5B genes were studied by DNA mixed pool PCR-SSCP DNA sequencing, and the genetic structure, genetic diversity and growth traits of different goat breeds were analyzed. In order to obtain the molecular genetic assistant markers which can improve the individual growth traits of goat breeds. At the same time, Q PCR technique was used to analyze the expression of STAT5A and STAT5B genes in 9 different tissues of Guizhou White Goat. The results were as follows: 1. Three SNPs2 STAT5A C11447T loci were screened in three goat STAT5A and STAT5B genes, respectively, which had a significant effect on the body height of Guizhou White Goat and the weight of Guizhou Black Goat was affected by G14264A locus. Body height and chest circumference had significant effects on body weight, body length, tube circumference and chest circumference of Guizhou white goats and body weight and chest circumference of Guizhou black goats. The T16159C locus of P0.05 STAT5B gene had significant effects on body weight, body length, tube circumference and chest circumference of Guizhou white goats and body weight of Qianbei Ma sheep. Body height, tube circumference and chest circumference had significant effects on body weight, body oblique length, tube circumference and chest circumference of Guizhou black goat, and C21455T locus on five growth traits of Guizhou white goat. Weight and chest circumference of Guizhou Black Goat and weight of Guizhou Black Goat, Body height and body oblique length have significant effects on the expression of P0.05 STAT5A and STAT5B genes in Guizhou White Goat at different age stages: liver, pituitary, heart, hypothalamus, lung, spleen, cerebellum, kidney, adult sheep: cardiopulmonary pituitary, hypothalamus, cerebellum, spleen, liver, kidney, liver and kidney. ST5B: liver, pituitary, heart, spleen, cerebellar, cerebellar, cerebellar and renal hypothalamus, adult sheep: cardiopulmonary cerebrum, liver, pituitary, kidney, cerebellar hypothalamus, liver, lung, cerebrum, pituitary, kidney and cerebellar hypothalamus showed the highest expression in Guizhou white goat, liver and pituitary. The expression of these two genes in the heart, lungs and cerebrum of Guizhou white goat is the highest, suggesting that these two genes are important genes to regulate the early growth of goats, and their main roles and action sites change with the development and maturation of goat individuals. At the same time, the two genes have a high degree of coordination and consistency.
【學(xué)位授予單位】：貴州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S827

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