發(fā)布時間：2018-05-05 15:28

  本文選題：雞血細(xì)胞 + 利巴韋林��； 參考：《中國畜牧獸醫(yī)》2017年10期

【摘要】：試驗旨在建立雞血細(xì)胞藥物代謝模型,并用利巴韋林進(jìn)行驗證。本試驗比較了3種不同培養(yǎng)基(L-15、M199和RPMI1640培養(yǎng)基)及添加不同濃度胎牛血清的細(xì)胞培養(yǎng)效果,采用MTT法測細(xì)胞活力,確定最佳給藥時間,之后用利巴韋林進(jìn)行給藥,檢測培養(yǎng)液中利巴韋林及其代謝物(TCONH2和RTCOOH)含量。結(jié)果發(fā)現(xiàn),3種培養(yǎng)基中用L-15培養(yǎng)基培養(yǎng)時細(xì)胞存活率最高,胎牛血清添加濃度為10%時血細(xì)胞狀態(tài)較好;血細(xì)胞活力檢測表明其最佳給藥時間為培養(yǎng)3 h;利巴韋林給藥后,其含量隨著時間的延長而降低,TCONH2和RTCOOH在給藥0.5 h時迅速產(chǎn)生,給藥3 h后其濃度變化趨于平緩。綜上所述,本試驗建立的雞血細(xì)胞代謝模型操作簡便,用添加10%胎牛血清的L-15培養(yǎng)基培養(yǎng)效果較好,利巴韋林給藥試驗表明,雞血細(xì)胞存在一定的代謝轉(zhuǎn)化功能,該雞血細(xì)胞代謝模型可用于某些藥物的體外代謝研究。
[Abstract]:The aim of this study was to establish a drug metabolism model of chicken blood cells and to verify it with ribavirin. In this experiment, we compared the cell culture effects of three different media (L-15M199 and RPMI1640) and supplemented with different concentrations of fetal bovine serum. MTT method was used to measure cell viability and determine the best administration time, and then ribavirin was given. The contents of ribavirin and its metabolites TCONH2 and RTCOOH were determined. The results showed that the cell survival rate was the highest in L-15 medium, the blood cell status was better when the serum concentration of fetal cattle was 10, the best time of administration was 3 h, the best time was 3 h after ribavirin administration. The content of TCONH2 and RTCOOH decreased rapidly with the increase of time, and the concentration of TCONH2 and RTCOOH increased slowly after 3 h of administration. To sum up, the model of chicken blood cell metabolism established in this experiment is simple and easy to operate, and the culture effect of L-15 medium supplemented with 10% fetal bovine serum is better. Ribavirin administration test shows that chicken blood cell has certain metabolic transformation function. The model of chicken blood cell metabolism can be used to study the metabolism of some drugs in vitro.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院農(nóng)業(yè)質(zhì)量標(biāo)準(zhǔn)與檢測技術(shù)研究所 農(nóng)業(yè)部農(nóng)產(chǎn)品質(zhì)量安全重點實驗室;
【基金】：中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項目
【分類號】：S859.8
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本文編號：1848226