本文選題：雙峰駝 + MHC-DRB基因�。� 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：目的：本研究通過探索內(nèi)蒙古阿拉善右旗、阿拉善左旗、鄂爾多斯市、巴彥淖爾市、錫林郭勒盟地區(qū)的雙峰駝群體MHC-DRB基因第二外顯子序列多態(tài)性；分析了阿拉善雙峰駝MHC-DRB基因與陰道蠅蛆病的相關(guān)性；研究了阿拉善雙峰駝和蘇尼特雙峰駝兩個(gè)品種遺傳多樣性,并判斷其親緣關(guān)系,旨在為以后雙峰駝的抗病育種提供重要的信息。方法：(1)對(duì)內(nèi)蒙古地區(qū)121峰雙峰駝MHC-DRB基因外顯子2進(jìn)行克隆測序,用不同分析軟件進(jìn)行序列分析,確定多態(tài)位點(diǎn)、等位基因種類、氨基酸變異位點(diǎn)等。(2)采用PCR產(chǎn)物直接測序的方法檢測了40峰患陰道蠅蛆病的阿拉善雙峰駝和54峰未患陰道蠅蛆病的阿拉善雙峰駝的MHC-DRB基因第二外顯子序列,并計(jì)算了患病駝和未患病駝的基因頻率和基因型頻率。(3)采用PCR產(chǎn)物直接測序的方法檢測了72峰阿拉善雙峰駝和25峰蘇尼特雙峰駝MHC-DRB基因外顯子2序列多態(tài)性；用分析軟件計(jì)算97峰雙峰駝序列的遺傳多樣性參數(shù)值,NJ法構(gòu)建兩品種系統(tǒng)發(fā)育樹。結(jié)果：(1)運(yùn)用分析軟件檢測了121峰雙峰駝DRB基因第二外顯子序列,共發(fā)現(xiàn)了225個(gè)突變位點(diǎn)、213個(gè)多態(tài)位點(diǎn)；36種等位基因；84個(gè)氨基酸中有62個(gè)變異位點(diǎn),其中包括18個(gè)假定抗原結(jié)合位點(diǎn)。(2)通過對(duì)阿拉善雙峰駝中患蠅蛆病駝和健康駝序列分析,發(fā)現(xiàn)都由兩個(gè)不同的基因控制；健康駝?dòng)腥N基因型,患病駝?dòng)袃煞N基因型。(3)通過軟件分析了72峰阿拉善雙峰駝和25峰蘇尼特雙峰駝序列,發(fā)現(xiàn)阿拉善雙峰駝?dòng)腥N單倍型,蘇尼特雙峰駝?dòng)袃煞N單倍型。(4)對(duì)93和159多態(tài)位點(diǎn)進(jìn)行哈迪-溫伯格平衡檢驗(yàn),發(fā)現(xiàn)P值均小于0.05；(5)雙峰駝MHC-DRB基因序列進(jìn)行Tajima's檢驗(yàn),Tajima'sD值為正值,P0.01；(6)兩個(gè)雙峰駝品種的MHC-DRB基因外顯子2序列構(gòu)建系統(tǒng)發(fā)育樹,大多數(shù)阿拉善雙峰駝聚在一支,蘇尼特雙峰駝聚在另一支上,也有一部分兩品種雙峰駝混合分布在一支上。結(jié)論：MHC-DRB基因在五個(gè)地區(qū)雙峰駝群體中具有豐富的多態(tài)性；阿拉善雙峰駝中患病駝和健康駝與MHC-DRB基因有一定的相關(guān)性；阿拉善雙峰駝和蘇尼特雙峰駝MHC-DRB基因外顯子2序列都存在遺傳多樣性,且蘇尼特雙峰駝的遺傳多樣性高于阿拉善雙峰駝;兩品種雙峰駝?dòng)H緣關(guān)系非常近。
[Abstract]:Objective: to explore the polymorphism of the second exon of MHC-DRB gene in Bactrian camel population in Inner Mongolia Alashan right Banner, Alashan Zuo Banner, Ordos City, Bayan Nur City and Xilingol region. The relationship between MHC-DRB gene and vaginal myiasis in Alashan Bactrian Camel was analyzed, the genetic diversity of Alashan Bactrian Camel and Sunita Bactrian Camel was studied, and the genetic relationship was judged. The aim is to provide important information for disease resistance breeding of Bactrian camel in the future. Methods: 1) cloning and sequencing of exon 2 of MHC-DRB gene of 121-peak bactrian camel in Inner Mongolia. The polymorphic loci and alleles were determined by using different analysis software. Amino acid mutation site et al. (2) the MHC-DRB gene exon 2 of Alashan bactrian camel with 40 peaks of vaginal myiasis and 54 peaks without vaginal myiasis was detected by direct sequencing of PCR products. The gene frequency and genotype frequency of infected and uninfected camels were calculated. The polymorphism of exon 2 of MHC-DRB gene of Alashan Bactrian Camel and 25 Sunita Bactrian Camel was detected by direct sequencing of PCR products. The genetic diversity parameter values of 97 peak bactrian camel sequence were calculated by using the analysis software and NJ method was used to construct the phylogenetic tree of two varieties. Results the second exon sequence of DRB gene of Bactrian Camel was detected by using analysis software. A total of 225 mutation sites and 36 alleles of 213 polymorphic loci were found, and 62 mutation sites were found in 84 amino acids. This includes 18 hypothetical antigen-binding sites.) by analyzing the sequences of myiasis and healthy camels in Alashan Bactrian Camels, we found that they were both controlled by two different genes; healthy camels had three genotypes. The disease camels have two genotypes.) by software analysis of 72 peak Alashan bactrian camel and 25 peak Sunita bactrian camel sequence, we found that there are three haplotypes in Alashan bactrian camel. Sunita Bactrian Camel has two haplotypes, I. e., Hadi Weinberg equilibrium test for 93 and 159 polymorphic loci. It was found that the MHC-DRB gene sequence of Bactrian Camel (P < 0.05) was detected by Tajima's. The MHC-DRB gene exon 2 sequence of two Bactrian camel varieties was constructed by Tajima's test. Most Alashan bactrian camels were clustered in the same tree. Sunita bactrian camels gather on the other, and some of the two species are mixed in one. Conclusion there is a rich polymorphism in the population of Bactrian Camel (Bactrian Camel) in five regions, and there is a certain correlation between the MHC-DRB gene and the diseased camel and healthy camel in Alashan Bactrian Camel. There was genetic diversity in exon 2 of MHC-DRB gene in Alashan Bactrian camel and Sunita Bactrian Camel, and the genetic diversity of Sunita Bactrian Camel was higher than that of Alashan Bactrian Camel.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S824

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 閆路娜,張德興;種群微衛(wèi)星DNA分析中樣本量對(duì)各種遺傳多樣性度量指標(biāo)的影響[J];動(dòng)物學(xué)報(bào);2004年02期

2 陳幼春,曹紅鶴,李宏濱;品種內(nèi)亞群定點(diǎn)隨機(jī)抽樣法的應(yīng)用研究[J];黃牛雜志;2001年01期

3 禹文海;魯紹雄;;牛MHC(BoLA)基因的研究進(jìn)展[J];中國牛業(yè)科學(xué);2007年01期

4 權(quán)潔霞,韓建林,門正明,張亞平,張國琪,魯守瑋;雙峰駝線粒體DNA地高辛標(biāo)記探針的制備及其應(yīng)用[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報(bào);1997年04期

5 邱林;趙武述;;人類MHC區(qū)域基因結(jié)構(gòu)研究進(jìn)展[J];國外醫(yī)學(xué)(免疫學(xué)分冊(cè));1998年01期

6 張?zhí)祛?劉至治;劉俊;趙雪錦;王成輝;;玻璃紅鯉MHCⅡ類基因多態(tài)性與組織表達(dá)分析[J];淡水漁業(yè);2013年03期

7 亢孝珍;額爾敦木圖;姜建強(qiáng);陳澤明;劉圖雅;伊特格勒?qǐng)D;沙日扣;圖雅;;主要組織相容性復(fù)合體(MHC)基因研究進(jìn)展[J];中國畜牧獸醫(yī);2014年05期

8 陳智華;顧磊;鐘金城;;德昌水牛Bola-DRB3基因第二外顯子的PCR-RFLP多態(tài)性研究[J];家畜生態(tài)學(xué)報(bào);2007年06期

9 袁俊華,孫成剛;山東地區(qū)慢乙肝的預(yù)后與HLA-DRB1等位基因相關(guān)性研究[J];臨床肝膽病雜志;2004年04期

10 劉丑生,王志剛,李寧;我國瀕危畜禽遺傳資源保護(hù)的現(xiàn)狀與對(duì)策[J];中國牧業(yè)通訊;2004年21期

相關(guān)碩士學(xué)位論文 前5條

1 杜丹丹;川西北獼猴MHC-DRB基因外顯子2多態(tài)性研究[D];四川農(nóng)業(yè)大學(xué);2011年

2 王準(zhǔn);小鼠H2－Eb位點(diǎn)多態(tài)性與特異性體液免疫應(yīng)答[D];大連醫(yī)科大學(xué);2003年

3 張瑋;四川獼猴MHC DRB等位基因外顯子2多樣性分析[D];四川農(nóng)業(yè)大學(xué);2009年

4 柏麗;中國6個(gè)雙峰駝群體微衛(wèi)星遺傳多樣性分析[D];寧夏大學(xué);2014年

5 陳月娥;中國美利奴羊MHC-DRB1 exon2單倍型構(gòu)建及與布魯氏菌病易感性關(guān)聯(lián)性[D];石河子大學(xué);2014年

，

本文編號(hào)：1846025