本文選題：日本乙型腦炎病毒 + MDSCs　； 參考：《華中農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：日本乙型腦炎(Japanese encephalitis,JE)是由日本乙型腦炎病毒(Japanese encephalitis virus,JEV)引起的一種人畜共患傳染病,簡(jiǎn)稱乙腦。JEV為嗜神經(jīng)病毒,主要通過蚊蟲傳播。該病毒感染宿主后,經(jīng)外周免疫逃逸,能穿越血腦屏障從而進(jìn)入中樞神經(jīng)系統(tǒng)(central nervous system,CNS),引起腦內(nèi)炎癥風(fēng)暴,最終導(dǎo)致人和動(dòng)物的發(fā)病、死亡。JEV經(jīng)外周免疫逃逸進(jìn)入CNS是引起乙腦發(fā)病的主要原因,但對(duì)于JEV外周免疫逃逸的機(jī)制仍不清楚。本研究以感染乙腦病毒強(qiáng)毒株JEV-P3的C57BL/6小鼠為模型,研究JEV外周免疫逃逸過程中,MDSCs發(fā)揮的功能。具體研究?jī)?nèi)容如下:首先建立合適的感染模型,以105 PFU感染劑量尾靜脈注射C57BL/6小鼠,從感染后第1天到第5天,隔天采取小鼠血清及脾臟組織,用于MDSCs相關(guān)細(xì)胞因子m RNA水平檢測(cè)及流式細(xì)胞術(shù)檢測(cè)。結(jié)果發(fā)現(xiàn)在小鼠感染第3天,脾臟中MDSCs數(shù)量明顯上升的同時(shí),其發(fā)揮抑制功能相關(guān)的細(xì)胞因子表達(dá)也有所上調(diào)。用ELISA方法檢測(cè)Arg-1,我們發(fā)現(xiàn),在JEV刺激下,MDSCs所表達(dá)的Arg-1有明顯的上升趨勢(shì),而Arg-1是MDSCs發(fā)揮抑制功能的一個(gè)重要的因子;相對(duì)熒光定量PCR方法檢測(cè)與T細(xì)胞分化相關(guān)的細(xì)胞因子,發(fā)現(xiàn)MDSCs增殖的同時(shí),T細(xì)胞的分化被抑制;流式細(xì)胞術(shù)檢測(cè)MDSCs上ADAM17蛋白和T細(xì)胞上CD62L分子表達(dá)情況,結(jié)果顯示:ADAM17上調(diào)的同時(shí)CD62L表達(dá)量下降。綜上,本研究得出:乙腦病毒感染小鼠后能夠在使得外周免疫器官脾臟中的免疫抑制性細(xì)胞群體MDSCs數(shù)量上升,且刺激其發(fā)揮免疫抑制功能,從而協(xié)助JEV逃脫外周免疫應(yīng)答,最終進(jìn)入CNS,感染神經(jīng)元細(xì)胞。
[Abstract]:Japanese encephalitis Japanese encephalitis (JE) is a zoonotic infectious disease caused by Japanese encephalitis virus Japanese encephalitis virus. After infecting the host, the virus escapes through peripheral immunity and can cross the blood-brain barrier to enter the central nervous system central nervous system, causing inflammatory storms in the brain and eventually leading to the onset of human and animal disease. Death. JEV escape through peripheral immunity into CNS is the main cause of encephalitis B, but the mechanism of JEV peripheral immune escape is still unclear. In this study, C57BL/6 mice infected with Japanese encephalitis virus (JEV-P3) strain were used as models to study the function of JEV during peripheral immune escape. The specific contents of the study were as follows: firstly, a suitable infection model was established. C57BL/6 mice were injected by tail vein at a dose of 105 PFU. From the first day to the fifth day after infection, mice serum and spleen tissue were taken. It was used to detect the level of MDSCs related cytokine m RNA and flow cytometry. The results showed that the number of MDSCs in spleen increased significantly on the 3rd day of infection, and the expression of cytokines related to its inhibitory function was also up-regulated. ELISA assay was used to detect Arg-1. We found that the expression of Arg-1 in JEV stimulated by JEV showed an obvious upward trend, while Arg-1 was an important factor in the inhibition of MDSCs, and the relative fluorescence quantitative PCR method was used to detect the cytokines associated with T cell differentiation. The expression of ADAM17 protein on MDSCs and CD62L on T cell was detected by flow cytometry. In conclusion, this study concluded that the number of immunosuppressive cell population MDSCs in peripheral immune organs was increased in mice infected with Japanese encephalitis virus, and the immunosuppressive function was stimulated to assist JEV to escape the peripheral immune response. It eventually enters CNS and infects neuronal cells.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S852.65

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 趙榮樂;鄭光宇;;乙型腦炎病毒研究進(jìn)展[J];生物學(xué)通報(bào);2007年07期

2 鄧永;徐高原;;豬乙型腦炎研究進(jìn)展[J];養(yǎng)殖與飼料;2007年06期

相關(guān)博士學(xué)位論文 前1條

1 李么明;乙型腦炎病毒與樹突狀細(xì)胞的互作研究[D];華中農(nóng)業(yè)大學(xué);2009年

，

本文編號(hào)：1845828