本文選題：水牛 + FADS基因　； 參考：《中國畜牧獸醫(yī)》2017年10期

【摘要】：試驗旨在利用電子克隆法對水牛Δ6脂肪酸脫氫酶(Δ6-fatty acid desaturases,FADS2)基因進(jìn)行克隆和生物信息學(xué)分析,為探究FADS2基因?qū)λＣ谌樾阅艿淖饔脵C制奠定基礎(chǔ)。以奶牛FADS2基因序列(Gen Bank登錄號:NM_001083444.1)為探針設(shè)計引物,利用電子克隆法克隆水牛FADS2基因,并通過RT-PCR驗證,對FADS2基因的序列特征進(jìn)行生物信息學(xué)分析。測序結(jié)果表明,水牛FADS2基因序列全長為36 600 bp,由12個外顯子和11個內(nèi)含子組成,包含一個長1 335 bp的開放閱讀框,可編碼444個氨基酸。序列同源性分析顯示,水牛FADS2基因編碼序列與牦牛、黃牛、人、豬、家兔、虎鯨和褐家鼠序列的同源性分別為98.88%、98.88%、89.66%、90.79%、90.85%、92.35%和87.11%。蛋白質(zhì)預(yù)測分析表明,水牛FADS2蛋白分子質(zhì)量為52.51 ku,理論等電點(p I)為8.75,呈弱堿性,屬于親水性蛋白,無信號肽。系統(tǒng)進(jìn)化樹分析結(jié)果表明,FADS2基因在不同物種及進(jìn)化的過程中具有高度保守性,其中水牛與牦牛、黃牛親緣關(guān)系較近,與褐家鼠親緣關(guān)系較遠(yuǎn)。水牛FADS2基因的成功克隆為今后闡明水牛泌乳性能的作用機制奠定了基礎(chǔ)。
[Abstract]:The aim of this study was to clone the 螖 6 fatty acid dehydrogenase (螖 6-fatty acid desaturus FADS2) gene from buffalo by electronic cloning and to analyze its bioinformatics, so as to lay a foundation for exploring the mechanism of FADS2 gene acting on buffalo lactation performance. FADS2 gene sequence Gen Bank accession number: NM0083444.1) was used as a probe to design primers to clone buffalo FADS2 gene by electronic cloning, and the sequence characteristics of FADS2 gene were analyzed by bioinformatics through RT-PCR verification. The sequencing results showed that the buffalo FADS2 gene was 36,600 BP in length and consisted of 12 exons and 11 introns, which contained an open reading frame of 1 335 BP and encoded 444 amino acids. Sequence homology analysis showed that the homology of buffalo FADS2 gene coding sequence with yak, yellow cattle, human, pig, rabbit, orcas and brown rat sequences was 98.8888, 98.888.88 and 89.66, respectively. The homology of buffalo FADS2 gene coding sequence was 90.85% and 92.35% and 87.11% respectively with yak, yellow cattle, human being, rabbit and Rattus norvegicus. Protein prediction and analysis showed that the molecular weight of buffalo FADS2 protein was 52.51 kuand the theoretical isoelectric point (Ip) was 8.75, which was weakly alkaline and belonged to hydrophilic protein with no signal peptide. Phylogenetic tree analysis showed that the FADS2 gene was highly conserved in different species and evolution, among which buffalo was closely related to yak and yellow cattle, and far related to Rattus norvegicus (Rattus norvegicus). The successful cloning of buffalo FADS2 gene laid a foundation for elucidating the mechanism of buffalo lactation.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院廣西水牛研究所 農(nóng)業(yè)部(廣西)水牛遺傳繁育重點實驗室;
【基金】：廣西科技重大專項(桂科AA16450002) 廣西科技公關(guān)國合項目(桂科合1504001-3) 廣西自然科學(xué)基金青年項目(2015GXNSFBA139103) 廣西水產(chǎn)畜牧科技推廣應(yīng)用項目(桂漁牧科201633009)
【分類號】：S823.83
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本文編號：1844843