本文選題：豬圓環(huán)病毒型 + 聚合酶鏈?zhǔn)椒磻?yīng)��； 參考：《中國獸醫(yī)科學(xué)》2017年12期

【摘要】：根據(jù)NCBI中PCV3的基因組序列,設(shè)計(jì)了1對特異性引物,并對PCR的反應(yīng)體系和反應(yīng)條件進(jìn)行優(yōu)化,同時(shí)進(jìn)行特異性和敏感性試驗(yàn),最后應(yīng)用所建立的PCR方法檢測了臨床送檢的22份病料。結(jié)果顯示,建立的PCR方法的最佳反應(yīng)條件為總體積25 L,退火溫度56℃,模板DNA量3 L(l ng/m L),進(jìn)行35個(gè)循環(huán)。該P(yáng)CR能特異地?cái)U(kuò)增出目的片段,長度約為381 bp。測序結(jié)果表明,PCR擴(kuò)增產(chǎn)物正確。該P(yáng)CR反應(yīng)特異性強(qiáng),只能擴(kuò)增PCV3陽性樣品,且能擴(kuò)增的最低模板量為1×10-4ng/m L。用該P(yáng)CR對送檢的22份病料進(jìn)行PCV3檢測,結(jié)果陽性率為13.6%�？梢�,本研究建立的PCR方法特異、敏感,可用于PCV3的快速檢測。
[Abstract]:According to the genomic sequence of PCV3 in NCBI, a pair of specific primers was designed, and the reaction system and reaction conditions of PCR were optimized, and the specificity and sensitivity tests were carried out. Finally, 22 samples were detected by the established PCR method. The results show that the optimum reaction conditions of the PCR method are: total volume 25 L, annealing temperature 56 鈩，

本文編號：1826582