本文選題：豬氣喘病 + 診斷　； 參考：《南京農(nóng)業(yè)大學》2015年碩士論文

【摘要】：豬氣喘病是由豬肺炎支原體(Mycoplasmahyopneumoniae,Mhp)感染引起的一種慢性呼吸道傳染病,以咳嗽、喘氣和生長發(fā)育遲緩為主要臨床特征。該病極難防控,給養(yǎng)豬業(yè)帶來巨大的損失。如何經(jīng)濟有效的控制豬氣喘病一直是困擾全球養(yǎng)豬業(yè)的難題,目前研究表明控制該病的最好方法是從源頭抓起徹底凈化氣喘病病原。本研究在結(jié)合我們研究室對豬肺炎支原體防控及致病機理研究成果的基礎上,借鑒前人的研究經(jīng)驗,通過氣喘病陽性豬群的診斷,以氣喘病陽性母豬為基礎,采用集成聯(lián)合用藥、隔離早期斷奶(SEW)和"三點式"生產(chǎn)體系的培育方法,成功建立了 190頭氣喘病陰性群豬。主要研究內(nèi)容如下:1、豬氣喘病陽性豬群的診斷:選擇疑似感染豬肺炎支原體且正在發(fā)病的某養(yǎng)殖場,通過流行病學調(diào)查、臨床癥狀觀察、咳嗽指數(shù)統(tǒng)計分析、剖解評分、病原分離培養(yǎng)鑒定、血清抗體監(jiān)測及鼻拭子中抗原熒光定量PCR方法檢測等實驗室診斷結(jié)果綜合分析,確診該場感染了豬肺炎支原體并發(fā)病,為后期氣喘病的凈化方法及監(jiān)控診斷得以科學、有效的實施奠定基礎。2、氣喘病陰性群豬的建立:本研究以上述豬群為基礎,先通過妊娠母豬的篩選,母豬程序性用藥,再通過SEW技術、屏障隔離系統(tǒng)、獨立飼養(yǎng)管理體系及仔豬程序性用藥的培育方法,培育獲得5批共190頭豬,通過豬肺炎支原體血清抗體檢測和熒光定量PCR檢測鼻拭子中抗原檢測,持續(xù)4個月的檢測,證明新培育的5批豬在35日齡后血清抗體均為陰性,鼻拭子中抗原檢測全為陰性,表明集成聯(lián)合運藥、SEW和"三點式"生產(chǎn)管理體系技術可以有效凈化豬肺炎支原體,為國內(nèi)豬氣喘病的控制和凈化提供理論基礎及臨床實踐經(jīng)驗。
[Abstract]:Swine asthma is a chronic respiratory infectious disease caused by Mycoplasma pneumoniae pneumoniae (Mhp) infection. Cough, wheezing and growth retardation are the main clinical features. The disease is extremely difficult to prevent and control, which brings huge losses to the pig industry. How to control asthma in pigs economically and effectively has always been a difficult problem in pig industry all over the world. Current studies show that the best way to control the disease is to thoroughly purify the pathogen of asthma from the source. Based on the research results of prevention and control of mycoplasma pneumoniae and pathogenesis of mycoplasma pneumoniae in our laboratory, and based on the previous research experience, the diagnosis of positive pigs with asthma disease was carried out, and the results were based on the positive sows with asthma disease. 190 pigs with negative asthma disease were successfully established by using the method of integrated drug use, isolating SEW (early weaning) and "three-point" production system. The main contents of the study are as follows: 1, diagnosis of swine asthma positive pigs: select a breeding farm that is suspected to be infected with Mycoplasma pneumoniae and is developing. Through epidemiological investigation, clinical symptom observation, cough index statistical analysis, dissection score, Comprehensive analysis of laboratory diagnostic results such as pathogen isolation and culture identification, serum antibody monitoring and antigen fluorescence quantitative PCR detection in nasal swab showed that mycoplasma pneumoniae was infected in this field. For the later stage of asthma disease purification methods and monitoring diagnosis can be scientific, effective implementation of the foundation of the establishment of asthma negative pigs: this study is based on the above pig herd, first through the screening of pregnant sows, sows program medication, Then through SEW technology, barrier isolation system, independent feeding management system and breeding method of Piglet programmed medication, 5 batches of 190 pigs were obtained. The detection of antibody to mycoplasma pneumoniae in serum and detection of antigen in nasal swab by fluorescence quantitative PCR for 4 months proved that all the 5 groups of pigs were negative in serum and negative in antigen of nasal swab after 35 days of age. The results show that the integrated technology of SEW and "three-point" production management system can effectively purify mycoplasma pneumoniae and provide theoretical basis and clinical experience for the control and purification of swine asthma disease in China.
【學位授予單位】：南京農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.28

【參考文獻】

相關期刊論文 前10條

1 于新友;李天芝;王金良;李書光;苗立中;沈志強;;豬支原體肺炎常用實驗室診斷方法概述[J];今日畜牧獸醫(yī);2014年12期

2 甘源;華利忠;熊祺琰;馬慶紅;徐飛揚;劉茂軍;杜改梅;武昱孜;韋艷娜;馮志新;王海燕;白方方;邵國青;;集成聯(lián)合用藥、隔離早期斷奶(SEW)和“三點式”生產(chǎn)體系培育豬氣喘病陰性群[J];江蘇農(nóng)業(yè)科學;2014年07期

3 白方方;武昱孜;靳蒙蒙;劉茂軍;馮志新;熊祺琰;邵國青;;巢式PCR檢測豬鼻支原體方法的建立及應用[J];中國獸醫(yī)學報;2013年07期

4 馮志新;張亞;華利忠;甘源;韋艷娜;邵國青;;豬鼻拭子樣品采集及制備方法的標準化[J];中國農(nóng)學通報;2013年05期

5 張悅;劉茂軍;邵國青;;豬肺炎支原體主要抗原蛋白的研究進展[J];中國農(nóng)學通報;2013年02期

6 武昱孜;靳蒙蒙;白方方;張旭;華利忠;雷治海;邵國青;;豬肺炎支原體P97 TaqMan-BHQ熒光定量PCR檢測方法的建立及應用[J];中國獸醫(yī)科學;2012年12期

7 韋艷娜;白昀;孔猛;馮志新;劉茂軍;熊祺琰;白方方;吳敘蘇;邵國青;;豬肺炎支原體的分離方法[J];江蘇農(nóng)業(yè)學報;2012年01期

8 邵國青;;豬喘氣病的控制與凈化方法的建議[J];養(yǎng)豬;2010年06期

9 逯曉敏;馮志新;劉茂軍;吳敘蘇;甘源;張映;邵國青;;豬肺炎支原體套式PCR檢測方法的建立及應用[J];江蘇農(nóng)業(yè)學報;2010年01期

10 趙榮茂;姚建聰;;豬支原體肺炎血清學檢測技術及其應用[J];豬業(yè)科學;2006年11期

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