本文選題：鵝出血性多瘤病毒 + 櫻桃谷鴨��； 參考：《中國畜牧獸醫(yī)》2017年04期

【摘要】：為明確福建地區(qū)是否存在鵝出血性多瘤病毒(goose hemorrhagic polyomavirus,GHPV)感染,本研究對2016年福建省臨床送檢測19份鴨組織樣品進行GHPV檢測,結(jié)果從1例櫻桃谷鴨中檢測到GHPV感染陽性(記為GHPV-FJ201601株)。隨后根據(jù)GenBank中GHPV參考株序列特征,設(shè)計針對GHPV的VP3基因特異性引物,利用PCR技術(shù)擴增獲得GHPV的VP3基因片段。結(jié)果顯示,GHPV-FJ201601株的VP3基因全長為654bp,編碼217個氨基酸。對編碼的VP3蛋白分析發(fā)現(xiàn)其理論等電點為9.37,帶負電荷氨基酸為23個(Asp+Glu),帶正電荷氨基酸為28個(Arg+Lys);不穩(wěn)定指數(shù)為38.43,是一個穩(wěn)定蛋白;脂肪系數(shù)64.33,總平均疏水性指數(shù)為-0.744;該蛋白無典型的信號肽切割位點;其亞細胞定位類型為核定位;對其進行磷酸化分析發(fā)現(xiàn),該蛋白存在14個氨基酸磷酸化位點。核苷酸同源性分析顯示,不同來源GHPV代表株VP3基因相互之間核苷酸同源性均較高,均不低于99.7%。VP3基因遺傳進化結(jié)果顯示,GHPV-FJ201601株和鵝源GHPV分離株(匈牙利14234株與法國Toulouse Goose 2000株)處于同一遺傳進化分支,與鴨源分離株(法國Toulouse Muscovy Duck 2008株、法國Toulouse Mule Duck 2008和中國106株)卻處于不同的遺傳進化分支,但所有GHPV分離株VP3基因遺傳進化均較近。本研究首次證實福建地區(qū)櫻桃谷鴨群中存在GHPV感染,為豐富不同地區(qū)與宿主的GHPV分子流行病學(xué)數(shù)據(jù)提供參考。
[Abstract]:In order to find out whether goose hemorrhagic polytumour virus (Goose hemorrhagic polyomavirus) infection exists in Fujian province, 19 duck tissue samples from Fujian Province in 2016 were tested for GHPV. The results showed that GHPV infection was positive in one Cherry Valley duck (known as GHPV-FJ201601 strain). Then according to the sequence characteristics of GHPV reference strain in GenBank, the specific primers for VP3 gene of GHPV were designed, and the VP3 gene fragment of GHPV was amplified by PCR technique. The results showed that the VP3 gene of GHPV-FJ201601 strain was 654 BP, encoding 217 amino acids. The analysis of the encoded VP3 protein showed that the theoretical isoelectric point was 9.37, the negatively charged amino acid was 23 Amino acids and the positively charged Amino Acid was 28 Arg LysA, and the instability index was 38.43, which was a stable protein. The fat coefficient was 64.33, the total average hydrophobic index was -0.744; the protein had no typical signal peptide cleavage site; its subcellular localization type was nuclear localization; the phosphorylation analysis showed that there were 14 amino acid phosphorylation sites in the protein. Nucleotide homology analysis showed that the nucleotide homology of VP3 gene from different GHPV representative strains was high. The genetic evolution of GHPV-FJ201601 was not lower than that of 99.7%.VP3 gene. The results showed that 14234 strains of GHPV isolated from geese (14234 strains from Hungary and 2000 strains of Toulouse Goose from France) were in the same phylogenetic branch as duck isolates (2008 strains of Toulouse Muscovy Duck in France). In France, Toulouse Mule Duck 2008 and China 2008 were in different branches of genetic evolution, but all GHPV isolates had close genetic evolution of VP3 gene. This study first confirmed the existence of GHPV infection in Cherry Valley Duck population in Fujian Province, which provides a reference for enriching the molecular epidemiological data of GHPV in different regions and hosts.
【作者單位】： 福建省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所福建省畜禽疫病防治工程技術(shù)研究中心福建省禽病防治重點實驗室;
【基金】：國家水禽產(chǎn)業(yè)技術(shù)體系(CARS-43) 國家自然科學(xué)基金(31602068) 福建省畜禽疫病防控技術(shù)重大研發(fā)平臺(2014N2003) 福建省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所基金(MYQJ2015(S)-3) 福建省屬公益類項目(2015R1023-7) 福建省自然科學(xué)基金(2015J01114)
【分類號】：S852.65

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