本文選題：共軛亞油酸 + 初產(chǎn)母豬��； 參考：《中國農(nóng)業(yè)大學(xué)》2017年博士論文

【摘要】：論文通過兩個飼養(yǎng)試驗和兩個細胞試驗研究妊娠后期飼糧添加共軛亞油酸(Conjugated linoleicacid,CLA)對初產(chǎn)和經(jīng)產(chǎn)母豬繁殖性能、初乳產(chǎn)量和免疫球蛋白G(ImmunoglobulinG,IgG)含量的影響及其可能的分子作用機制。試驗一,選用48頭平均體重為257.6± 1.5 kg,平均胎次為5.5±0.1胎的經(jīng)產(chǎn)長白×大白雜交母豬,按體重和胎次相近的原則隨機分配到4個處理,每個處理12個重復(fù),每個重復(fù)1頭母豬,從妊娠第85 d到分娩前分別飼喂含0%(對照組)、0.75%、1.50%和2.25%CLA的試驗飼糧。結(jié)果表明,飼糧中添加共軛亞油酸對經(jīng)產(chǎn)母豬的窩產(chǎn)仔數(shù)、初乳產(chǎn)量及其仔豬的生長性能均無顯著影響。與對照組相比,添加共軛亞油酸提高妊娠第112d母豬血清(P0.05)、初乳(P0.01)以及新生仔豬血清(P0.01)中IgG含量,但添加不同CLA 水平的處理間沒有顯著差異。試驗二,選用24頭平均體重為170.7 ± 2.3 kg的初產(chǎn)長白×大白雜交母豬,按體重相近的原則分配到4個處理,每個處理6個重復(fù),每個重復(fù)1頭母豬,從妊娠第85d到分娩前分別飼喂含0%(對照組)、0.75%、1.50%和2.25%CLA的試驗飼糧。結(jié)果表明,與對照組相比,添加1.5%和2.25%CLA提高初產(chǎn)母豬初乳IgG的含量(P0.05),而添加2.25%CLA可以提高新生仔豬血清中IgG含量(P0.05),降低哺乳仔豬的死亡率(P0.05)。試驗三,選用不同濃度(0、12.5、25、50、100和200μM)的順9,反11(c9,t11)、反10,順12(t10,c12)共軛亞油酸以及兩者的等比例混合物對美洲商陸絲裂原刺激下豬外周血單核細胞(Peripheral blood mononuclear cells,PBMCs)的體外增殖率和IgG生成的影響。結(jié)果表明,t10,c12-CLA濃度為12.5和25 μM時促進PBMCs的增殖(P0.05)。當(dāng)c9,t11-CLA濃度為50μM以上,以及t10,c12-CLA和兩種CLA異構(gòu)體混合物的濃度為100 μM以上時,PBMCs的增殖受到抑制(P0.05)。另外,c9,t11-CLA、t10,c12-CLA及其混合物濃度分別為12.5、12.5和25、25 μM時促進豬外周血單核細胞IgG的生成(P0.05)。試驗四,在試驗三的基礎(chǔ)上進一步研究t10,c12-CLA 對豬 PBMCs 蛋白激酶 C(Protein kinase C,PKC)和細胞因子生成,以及 t10,c12-CLA 和 GW9508 對 IgG 生成和 G 蛋白偶聯(lián)受體 120(G-protein coupled receptor 120,GPR120)蛋白表達的影響。結(jié)果表明,t10,c12-CLA促進豬PBMCs白介素-2和PKC的生成(P0.05),抑制干擾素-γ的生成(P0.05)。t10,c12-CLA及其與GW9508的組合顯著提高豬外周血單核細胞IgG的生成(P0.05)。t10,c12-CLA、GW9508以及兩者的組合提高豬PBMCsGPR120蛋白的表達(P0.05)。結(jié)論,妊娠后期飼糧添加共軛亞油酸可以提高初產(chǎn)母豬和經(jīng)產(chǎn)母豬初乳IgG的含量,與c9,t11-CLA相比,t10,c12-CLA在促進IgG生成方面可能發(fā)揮更重要的作用。
[Abstract]:In this paper, the effects of conjugated linoleic acid on reproductive performance, colostrum yield and immunoglobulin G G (IgG) content in newborn and adult sows were studied by two feeding experiments and two cell tests. In experiment 1, 48 Landrace 脳 big white crossbred sows with average body weight of 257.6 鹵1.5 kg and average birth order of 5.5 鹵0.1 were randomly assigned to 4 treatments according to the principle of similar body weight and birth order, 12 replicates per treatment and 1 sow per replica. The experimental diet containing 0.75% and 1.50% 2.25%CLA was fed from the 85th day of pregnancy to the beginning of labor. The results showed that the addition of conjugated linoleic acid in diet had no significant effect on litter size, colostrum yield and growth performance of piglets. Compared with the control group, conjugated linoleic acid (CLA) increased the content of IgG in serum of sows (P0.05), colostrums (P0.01) and newborn piglets (P0.01) on the 12th day of gestation, but there was no significant difference between the treatments with different levels of CLA. In experiment 2, 24 Landrace 脳 big white crossbred sows with an average body weight of 170.7 鹵2.3kg were selected and assigned to four treatments according to the principle of similar body weight, six replicates per treatment and one sows per replica. The experimental diet containing 0.75% and 1.50% 2.25%CLA was fed from day 85 to delivery. The results showed that addition of 1.5% and 2.25%CLA could increase the content of IgG in colostrum and increase the content of IgG in the serum of newborn piglets, and decrease the mortality of lactating piglets. In experiment 3, the effects of cis-9, anti-11c9t11, anti-10, cis-12t10c12) conjugated linoleic acid and their mixtures on proliferation rate and IgG production of peripheral blood mononuclear cells of porcine peripheral blood monocytes stimulated by American pokeweed mitogen were studied. The results showed that the concentration of C12-CLA was 12.5 渭 M and 25 渭 M, which promoted the proliferation of PBMCs. When the concentration of c9t11-CLA was more than 50 渭 M and the concentration of t10c12-CLA and two CLA isomers was more than 100 渭 M, the proliferation of CLA was inhibited. In addition, the concentration of C9T11-CLAT10C12-CLA and its mixture were 12.5 and 2525 渭 M, respectively, to promote the production of IgG in porcine peripheral blood monocytes (P0.05). In experiment 4, on the basis of experiment 3, we further studied the effects of t10c12-CLA on the production of porcine PBMCs protein kinase (C(Protein kinase) and cytokines, and on the expression of IgG and G protein-coupled receptor (120(G-protein coupled receptor 120-GPR120) by t10c12-CLA and GW9508. The results showed that t10c12-CLA promoted the production of PBMCs interleukin-2 and PKC, inhibited the production of IFN- 緯 and its combination with GW9508 significantly increased the production of IgG in porcine peripheral blood mononuclear cells (P0.05N. T10C12-CLAGW9508) and the combination of the two increased the expression of porcine PBMCsGPR120 protein. Conclusion: supplementation of conjugated linoleic acid in diet in late pregnancy can increase the content of IgG in colostrum of first born sows and sows. Compared with c9t11-CLA, t10c12-CLA may play a more important role in promoting the production of IgG.
【學(xué)位授予單位】：中國農(nóng)業(yè)大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：S828.5

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