本文選題：豬鏈球菌 + 分離　； 參考：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：豬鏈球菌(Streptococcus suis,SS)是豬鏈球菌病主要病原菌,能夠引起豬的腦膜炎、敗血癥、心內(nèi)膜炎、關(guān)節(jié)炎和肺炎,導(dǎo)致全球養(yǎng)豬業(yè)出現(xiàn)嚴(yán)重經(jīng)濟(jì)損失,也是一種重要的人畜共患病原菌,具有公共衛(wèi)生學(xué)意義。本研究針對江西地區(qū)分離得到的豬鏈球菌的耐藥性、毒力基因型分布及血清型進(jìn)行檢測分析,以期為江西地區(qū)豬鏈球菌病防治奠定基礎(chǔ)。在2014年至2016年間,從江西省多個不同規(guī)模豬場,對疑似豬鏈球菌病的病豬進(jìn)行病料采集,通過細(xì)菌的分離培養(yǎng)、特異性PCR鑒定及測序比對,共得到62株豬鏈球菌,其中在肺臟分離率最高。多數(shù)豬鏈球菌病呈現(xiàn)與其它病原混合感染,主要為PRRSV和PCV-2。運(yùn)用PCR基因分型方法對62株豬鏈球菌的流行的1、2、7、9型進(jìn)行鑒定。其中豬鏈球菌1型8株,豬鏈球菌2型22株,豬鏈球菌9型5株,分別占12.9%、35.5%、8.1%,未知血清型占43.5%,未檢測到7型豬鏈球菌。表明江西省存在豬鏈球菌的多種血清型感染,其中豬鏈球菌2型為主要流行血清型。根據(jù)豬鏈球菌6種主要毒力基因(mrp、orf2、gdh、sly、fbps、epf),設(shè)計合成對應(yīng)引物序列,對62株豬鏈球菌進(jìn)行檢測,檢測結(jié)果為:gdh(62/100%),mrp(47/75.8%),epf(33/53.2%),fbps(36/58.1%),orf2(34/54.8%),sly(14/22.3%)。22株豬2型鏈球菌中各種毒力因子占比均高于平均水平,可分為10種基因型,gdh+mrp+epf+fbps+orf2+sly-和gdh+mrp+epf+fbps+orf2+sly+為主要基因型。選取16種臨床常用抗菌藥物,應(yīng)用K-B紙片瓊脂擴(kuò)散法,對62株豬鏈球菌進(jìn)行藥物敏感性試驗,結(jié)果顯示,豬鏈球菌江西分離株多重耐藥嚴(yán)重,多重耐藥分離株數(shù)為59(95.16%)。對四環(huán)素、紅霉素、強(qiáng)力霉素耐藥最為嚴(yán)重,分別占96.78%、80.64%和80.64%;對頭孢唑啉、頭孢噻肟和阿莫西林敏感度較高,分別占85.49%、87.1%和82.26%。表明豬鏈球菌在一定程度上對常用藥物產(chǎn)生了耐藥性。
[Abstract]:Streptococcus suis (Streptococcus suis, SS), the main pathogen of Streptococcus suis disease, can cause meningitis, septicemia, endocarditis, arthritis and pneumonia in pigs, causing serious economic losses in the global pig raising industry. It is also an important zoonosis, which has the significance of public hygiene. This study was isolated in Jiangxi area. The drug resistance, the distribution of virulence genotype and the serotype of Streptococcus suis were detected and analyzed in order to lay the foundation for the prevention and control of Streptococcus suis disease in Jiangxi. From 2014 to 2016, from various pig farms in Jiangxi Province, the diseases of pigs suspected to be Streptococcus suis were collected, the isolation and culture of bacteria, specific PCR identification and measurement were carried out. 62 strains of Streptococcus suis were obtained by sequence alignment, among which the lung separation rate was the highest. Most Streptococcus suis disease was mixed with other pathogens, mainly PRRSV and PCV-2. used PCR genotyping to identify the 62 strains of Streptococcus suis, including 8 strains of Streptococcus suis, 22 Streptococcus suis type 2, and 5 strains of Streptococcus suis 9, and 5 strains of Streptococcus suis. 12.9%, 35.5%, 8.1%, unknown serotypes accounted for 43.5%, and no Streptococcus suis type 7 was not detected. It showed that there were a variety of serotype infection of Streptococcus suis in Jiangxi Province, and Streptococcus suis 2 was the main serotype. According to the 6 main virulence genes of Streptococcus suis (MRP, ORF2, GDH, sly, FBPS, EPF), the sequence of corresponding primers was designed and synthesized, and 62 pig chains were designed. The test results were as follows: GDH (62/100%), MRP (47/75.8%), EPF (33/53.2%), FBPS (36/58.1%), ORF2 (34/54.8%), sly (14/22.3%) type 2 streptococci were all higher than the average level, and could be divided into 10 genotypes. 16 species were selected. 62 strains of Streptococcus suis were tested by K-B paper agar diffusion method. The results showed that the multidrug resistance of Streptococcus suis Jiangxi isolates was serious and the number of multidrug resistant isolates was 59 (95.16%). The most serious resistance to tetracycline, erythromycin, and doxycycline was 96.78%, 80.64% and 80.64%, respectively. The sensitivity of cephalosporin, cefotaxime and amoxicillin was 85.49%, 87.1% and 82.26%. respectively, indicating that Streptococcus suis was resistant to commonly used drugs.

【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S852.611

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