本文選題：秸稈 + 木質(zhì)纖維素　； 參考：《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2017年04期

【摘要】：本試驗(yàn)以水稻秸稈為研究對(duì)象,采用高效降解纖維素酶進(jìn)行青貯發(fā)酵處理,分析其對(duì)水稻秸稈微觀(guān)結(jié)構(gòu)的影響,旨在實(shí)現(xiàn)農(nóng)作物秸稈的高效降解,提高其營(yíng)養(yǎng)價(jià)值。設(shè)青貯對(duì)照(S組)、復(fù)合酶制劑處理(C組)、果膠酶+漆酶處理(PL組)、復(fù)合酶制劑+果膠酶+漆酶處理(CPL組)和原料(M組),各組采用袋裝真空青貯。室溫貯藏45 d后取樣,采用實(shí)驗(yàn)室檢測(cè)方法、苯酚硫酸法、4,4'二羧酸-2,2'-喹啉(BCA)法、近紅外光譜(FTIR)、X-射線(xiàn)衍射和伊紅美蘭法分析不同酶或酶組合處理的水稻秸稈青貯飼料的營(yíng)養(yǎng)成分含量、發(fā)酵品質(zhì)及微觀(guān)結(jié)構(gòu)變化。結(jié)果表明:1)與S組相比,PL組、C組及CPL組的乳酸(LA)含量顯著增加(P0.05),p H、氨態(tài)氮/總氮及乙酸、纖維素含量均顯著降低(P0.05);CPL組、C組的粗蛋白質(zhì)含量顯著降低(P0.05)。2)與S組相比,PL組青貯水稻秸稈的聚合度顯著降低(P0.05),降低幅度達(dá)30.26%,分子間氫鍵結(jié)合力減弱,結(jié)晶度與比表面積無(wú)顯著變化(P0.05);C組青貯水稻秸稈聚合度顯著降低(P0.05),降低幅度達(dá)27.11%,分子間氫鍵結(jié)合力減弱,結(jié)晶度與比表面積無(wú)顯著變化(P0.05);CPL組青貯水稻秸稈的聚合度顯著降低(P0.05),降低幅度達(dá)56.32%,分子間氫鍵結(jié)合力減弱,有降低結(jié)晶度和增加比表面積的趨勢(shì)(P0.05)。綜上,CPL組降解纖維組分的效果最理想,能有效地破解細(xì)胞壁中木質(zhì)素-纖維素-半纖維素復(fù)合結(jié)構(gòu),將纖維素降解為可利用的糖,提高水稻秸稈青貯飼料的營(yíng)養(yǎng)含量,降低聚合度及結(jié)晶度,增大比表面積,從而提高秸稈的可消化性及利用率。
[Abstract]:In this experiment, rice straw was used as the research object, and the effect of cellulase degradation on the microstructure of rice straw was analyzed in order to achieve the high efficiency degradation of crop straw and improve its nutritional value. The silage control group S, the compound enzyme treatment group C, the Pectinase laccase group, the compound enzyme Pectinase laccase group (CPL group) and the raw material group M were treated with vacuum silage in each group. After storage at room temperature for 45 days, the samples were sampled by laboratory method, and the method of phenol sulfuric acid 4 'dicarboxylic acid-2O2O2-Quinoline (BCA) was used. The content of nutrients, fermentation quality and microstructure of rice straw silage treated with different enzyme or enzyme combination were analyzed by near infrared spectrum FTIR X-ray diffraction and ehongmeilan method. The results showed that the content of lactic acid in group C and CPL increased significantly than that in group S (P 0.05), ammonia nitrogen / total nitrogen and acetic acid. Compared with group S, the degree of polymerization of silage rice straw in Pl group was significantly lower than that in group S, the degree of polymerization of silage rice straw was decreased significantly, the reduction range was 30.26%, and the hydrogen bonding force between the molecules was decreased, compared with that of group S, the content of cellulose was significantly decreased, and the content of crude protein in group C was significantly decreased compared with that of group S. There was no significant change in crystallinity and specific surface area. The degree of polymerization of silage straw significantly decreased by 27.11%, and the intermolecular hydrogen bond strength decreased. There was no significant change in crystallinity and specific surface area. The degree of polymerization of silage rice straw in P0.05 / CPL group was significantly decreased by 56.32cm, and the hydrogen bonding force between molecules was decreased. There was a tendency of decreasing crystallinity and increasing specific surface area (P0.05N). The effect of CPL group on the degradation of fiber components is the best, which can effectively decipher the lignin-cellulose hemicellulose compound structure in the cell wall, degrade cellulose into available sugar, and improve the nutrient content of rice straw silage. By reducing the degree of polymerization and crystallinity and increasing the specific surface area, the digestibility and utilization ratio of straw can be improved.
【作者單位】： 塔里木大學(xué)動(dòng)物科學(xué)學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院飼料研究所農(nóng)業(yè)部飼料生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：秸稈飼料生物轉(zhuǎn)化技術(shù)研究與示范項(xiàng)目(20120304202) 國(guó)家肉羊產(chǎn)業(yè)技術(shù)體系建設(shè)專(zhuān)項(xiàng)資金(CARS-39)
【分類(lèi)號(hào)】：S816

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