本文選題：小鵝瘟病毒 + 分子流行病學(xué)��； 參考：《仲愷農(nóng)業(yè)工程學(xué)院》2017年碩士論文

【摘要】：小鵝瘟是由小鵝瘟病毒(Goose Parvovirus,GPV)引起3~20日齡雛鵝和雛番鴨的一種急性或亞急性敗血性傳染病,具有傳播快、發(fā)病率高和死亡率高等特點(diǎn),給養(yǎng)鵝業(yè)造成了重大損失。為研究小鵝瘟在廣東省的流行情況,在2015年9月至2016年12月,從廣東省各地采集到有疑似小鵝瘟癥狀的鵝肝臟與腸組織42份,共分離到7株GPV,分別是從粵北地區(qū)清遠(yuǎn)市分離到的QY1和QY2,從珠三角地區(qū)廣州市分離到的GZ1和GZ2,從粵西地區(qū)陽江市分離到Y(jié)J1和YJ2,從粵西地區(qū)茂名市分離到的MM。對這7株GPV全基因進(jìn)行測序,并對其序列、遺傳進(jìn)化和糖基化位點(diǎn)進(jìn)行分析。結(jié)果顯示如下:在全基因分析中,7株GPV的全長都為5050 bp,它們之間的同源性為99.8%~99.9%,在進(jìn)化樹中形成一個獨(dú)立的新分支。7株GPV與GenBank上已發(fā)表的全基因序列同源性為93.4%~98.9%,與重慶毒株RC16親緣關(guān)系最近,同源性為98.8%~98.9%;與標(biāo)準(zhǔn)株B同源性為98.2%~98.3%。對NS進(jìn)行分析發(fā)現(xiàn),7株GPV的NS1和NS2分別長為1884 bp和456 bp,分別可翻譯成628和152個氨基酸。7株GPV的NS1的同源性為99.7%~100%,而NS2的同源性為100%。NS1只有個別堿基發(fā)生突變并引起氨基酸的變化,而NS2沒有發(fā)生堿基的突變。7株GPV在NS1進(jìn)化樹中形成一個獨(dú)立的新分支,與臺灣毒株06-0329、82-0321以及重慶毒株RC16親緣關(guān)系最為接近,同源性為99.1%~99.7%;與標(biāo)準(zhǔn)株B同源性為99.0%~99.2%。在NS2進(jìn)化樹中形成一個獨(dú)立的新分支,與臺灣毒株上海毒株SHFX1201和重慶毒株RC16親緣關(guān)系最為接近,同源性為99.1%~99.6%;與標(biāo)準(zhǔn)株B同源性為98.7%。7株GPV的NS有著4個相同的糖基化位點(diǎn),分別為150-152NKT、225-227NYS、360-362NWT、433-435NST,而GZ2比對比其他毒株多了330-332NAT糖基化位點(diǎn)。對VP進(jìn)行分析發(fā)現(xiàn),7株GPV的VP1、VP2和VP3分別長為2199 bp、1764 bp和1605 bp,分別可翻譯成733、588和535個氨基酸。VP1、VP2和VP3同源性分別為99.6%~100%、99.6%~99.9%和99.6%~99.9%。7株GPV在VP1進(jìn)化樹中形成一個獨(dú)立的新分支,與廣東毒株GDFSh以及重慶毒株RC16親緣關(guān)系最為接近,同源性為97.7%~98.2%;與標(biāo)準(zhǔn)株B同源性為97.4%~97.5%。在VP2進(jìn)化樹中形成一個獨(dú)立的新分支,與標(biāo)準(zhǔn)株B同源性為97.8%~97.9%。在VP3進(jìn)化樹中與江蘇毒株JSXZ-4和安徽毒株AH-1形成一個獨(dú)立的新分支,親緣關(guān)系最近,同源性為98.8%~99.8%;與標(biāo)準(zhǔn)株B同源性為97.9%~98.0%。7株GPV的VP有著5個相同的糖基化位點(diǎn),分別為219-221NAS、331-333NLT、582-584NTT、700-702NFS、712-714NET。對ITR進(jìn)行分析發(fā)現(xiàn),7株GPV的ITR都長為416 bp,它們間的同源性為99.8%~100%。7株GPV在進(jìn)化樹中形成一個獨(dú)立的新分支,與重慶毒株RC16和臺灣毒株06-0329親緣關(guān)系最近,同源性為97.0%~98.9%;與標(biāo)準(zhǔn)株B同源性為88.8%。本研究通過對從廣東分離到的7株GPV基因序列、遺傳進(jìn)化和糖基化位點(diǎn)進(jìn)行分析,旨在了解小鵝瘟病毒在廣東省的進(jìn)化方向和變異程度,為廣東小鵝瘟的防控提供數(shù)據(jù)依據(jù)。
[Abstract]:Gosling plague is an acute or subacute septic infectious disease caused by Goose Parvovirus (GPVV) of Gosling Plague virus (Goose Parvovirus). It is an acute or subacute septic infectious disease of goslings and young muscovy ducks at the age of 20 days. It has the characteristics of fast transmission, high incidence and high mortality, and has caused great losses to the goose industry. In order to study the prevalence of Gosling Plague in Guangdong Province, 42 goose liver and intestinal tissues with suspected symptoms of Gosling plague were collected from various parts of Guangdong Province from September 2015 to December 2016. Seven strains of GPVs were isolated from Qingyuan city in northern Guangdong, GZ1 and GZ2 from Guangzhou city in Pearl River Delta, YJ1 and YJ2-2 from Yangjiang city in western Guangdong, and MM. from Maoming city in western Guangdong. The whole GPV genes of the 7 strains were sequenced and their sequences, genetic evolution and glycosylation sites were analyzed. The results are as follows: in the whole gene analysis, the total length of GPV of the 7 strains is 5050 BP, the homology between them is 99.8 and 99.9. The homology between GPV and the published whole gene sequence published on GenBank is 93.4% 98.9, which is similar to that of the whole gene sequence published on GenBank, and the homology between them is 99.8% and 99.9% respectively. The RC16 strains of Chongqing are closely related to each other. The homology was 98.8% and 98.2% with the standard strain B. It was found that the NS1 and NS2 of GPV were 1884 BP and 456bp, respectively. The homology of NS1 in GPV of 628 and 152 amino acid 7 strains was 99.7%, while the homology of NS2 was that only a few base pairs of 100%.NS1 mutated and caused amino acid changes. However, GPV, which had no base mutation in NS2, formed an independent new branch in the NS1 evolutionary tree, most closely related to Taiwan strain 06-0329 (82-0321) and Chongqing strain (RC16), with the homology of 99.1% and 99.7%, and 99.0% and 99.2% with standard strain B. An independent branch was formed in the NS2 phylogenetic tree, most closely related to the Taiwan strain Shanghai strain SHFX1201 and Chongqing strain RC16 strain, with a homology of 99.1 and 99.6, and had four identical glycosylation sites with standard strain B homology of 98.7.7 strain GPV. The results were as follows: 150-152 NKTX 225-227NYSU 360-362NWTT 433-435NST, and GZ2 had more 330-332NAT glycosylation sites than other strains. The VP2 and VP3 of GPV were 2199 BP and 1605 BP, respectively, which could be translated into 733588 and 535 amino acids, respectively. The homology of VP2 and VP3 were 99.699% and 99.699.9.7% and 99.699.9.7%, respectively. The genetic relationship with Guangdong strain GDFSh and Chongqing strain RC16 is the closest, the homology is 97.798. 2, and the homology with standard strain B is 97.4 and 97.5. An independent branch was formed in the VP2 phylogenetic tree, and the homology with the standard strain B was 97.8% and 97.9%. An independent branch was formed in the VP3 phylogenetic tree with Jiangsu strain JSXZ-4 and Anhui strain AH-1, with the closest genetic relationship, the homology was 98.8% and 99.8%, and the VP of the standard strain B with 97.9% homology and 98.0.7% had five identical glycosylation sites, respectively, which were 219-221 NAS331-333NLTT 582-584NTT 700-702NFS 712-714NET. The results of ITR analysis showed that the ITR length of 7 GPV strains was 416bp. the homology among them was 99.8kW and 100.7 strains formed an independent new branch in the evolutionary tree, which was closely related to the Chongqing strain RC16 and Taiwan strain 06-0329. The homology was 97.0 and 98.9, and the homology with standard strain B was 88.8. In this study, the sequence, genetic evolution and glycosylation sites of 7 strains of GPV gene isolated from Guangdong province were analyzed in order to understand the evolutionary direction and variation of Gosling Plague virus in Guangdong Province, and to provide data basis for the prevention and control of Gosling Plague virus in Guangdong Province.
【學(xué)位授予單位】：仲愷農(nóng)業(yè)工程學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.33

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