本文選題：S- + 溶血性　； 參考：《中國動物傳染病學報》2017年04期

【摘要】：研究三七皂苷S-6的溶血性及體外免疫活性。常規(guī)方法提取PNS,大孔樹脂柱層析分離純化得S-6。溶血試驗測定紅細胞溶血率。脾淋巴細胞毒性試驗確定S-6的安全劑量范圍,體外脾淋巴細胞增殖反應和IL-2的誘生及含量測定檢測S-6的體外免疫活性。S-6溶血性較小,2 mg/mL濃度以下無溶血性;S-6(0.1μg/mL、1μg/mL)顯著促進了ConA誘導的小鼠脾淋巴細胞增殖反應,且與PNS和APS組相比,差異不顯著;S-6(1μg/mL)極顯著地促進了LPS誘導的小鼠脾淋巴細胞增殖反應,且與PNS相比,S-6(1μg/mL)差異顯著,其余各個劑量與PNS和APS組相比,差異不顯著;S-6(12.5μg/mL、25μg/mL、50μg/mL)顯著或極顯著地提高了IL-2的含量,且與PNS和APS組相比,差異不顯著。S-6安全性好,體外具有較好的細胞免疫活性和誘生細胞因子的能力。
[Abstract]:To study the hemolysis and in vitro immune activity of Panax notoginsenoside S-6. PNSs were extracted by conventional method and purified by macroporous resin column chromatography. The hemolysis rate of red blood cells was measured by hemolysis test. The safety dose range of S-6 was determined by splenic lymphocyte toxicity test. Proliferation of splenic lymphocytes in vitro and induction and determination of IL-2 in vitro the in vitro immunological activity of S-6. S-6 hemolytic activity. S-6 hemolytic activity was less than 2 mg/mL. No hemolytic S-6N 0.1 渭 g / mL 1 渭 g 路mL ~ (-1) significantly promoted the proliferation of spleen lymphocytes induced by ConA in mice. Compared with the PNS and APS groups, the difference was not significant (P < 0.05). The proliferation of splenic lymphocytes induced by LPS was significantly promoted by S-6N (1 渭 g / mL), and the difference was significant compared with that of PNS (P < 0.05). The other doses were compared with those of PNS and APS groups. There was no significant difference in S-6, 12.5 渭 g / mL, 25 渭 g 路mL ~ (-1) or 50 渭 g / mL). Compared with PNS and APS, the difference was not significant. S-6 was safe, and had better cellular immune activity and the ability to induce cytokines in vitro.
【作者單位】： 江蘇農(nóng)牧科技職業(yè)學院江蘇省獸用生物制藥高技術研究重點實驗室;揚州大學獸醫(yī)學院;
【基金】：江蘇省獸用生物制藥高技術研究中心重點實驗室開放基金資助項目 江蘇省產(chǎn)學研合作項目“新型中獸藥免疫增強劑復方桑杜口服液的研制”項目(BY2015066-01) 江蘇高�！扒嗨{工程”資助 “泰州市311人才工程”項目 學院“鳳凰人才工程”項目
【分類號】：S853.7

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