本文選題：小花棘豆 + 中毒�。� 參考：《江西農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年06期

【摘要】：以小花棘豆(Oxytropis glabra DC)中毒和田羊母羊?yàn)檠芯繉?duì)象,通過復(fù)制和田羊小花棘豆亞慢性中毒模型,對(duì)其卵巢的病理變化和超微結(jié)構(gòu)變化進(jìn)行觀察,檢測(cè)其氧化應(yīng)激和細(xì)胞凋亡情況,Real-Time q PCR法檢測(cè)繁殖基因mRNA的表達(dá)量,Western Blot檢測(cè)其蛋白表達(dá)量。結(jié)果發(fā)現(xiàn),小花棘豆中毒和田羊卵巢指數(shù)極顯著升高(P0.01),而且卵巢表面卵泡數(shù)極顯著下降(P0.01),鏡檢表明中毒和田羊卵巢中初級(jí)卵母細(xì)胞溶解、消失,間質(zhì)血管擴(kuò)張,原位末端標(biāo)記(TUNEL)發(fā)現(xiàn)中毒和田羊卵巢細(xì)胞和卵泡顆粒細(xì)胞發(fā)生凋亡,中毒和田羊卵巢Bax基因mRNA表達(dá)量極顯著升高(P0.01),Bcl-2基因mRNA表達(dá)量極顯著下降(P0.01)。小花棘豆中毒和田羊血清和卵巢組織中超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)和過氧化氫酶(CAT)活性極顯著下降(P0.01),丙二醛(MDA)含量極顯著升高(P0.01);血清中雌二醇(E2)和孕酮(P4)含量均極顯著下降(P0.01)。卵巢中FSHR和LHR mRNA表達(dá)量均極顯著下降(P0.01),其蛋白表達(dá)量均顯著下降(P0.05)。研究結(jié)果表明,小花棘豆中毒可導(dǎo)致和田羊卵巢病理性損傷,抑制相關(guān)繁殖基因表達(dá),促使細(xì)胞凋亡,從而抑制和田羊機(jī)體生殖功能。
[Abstract]:Taking Oxytropis glabra glabra poisoning and sheep sheep as the research object, the ovarian pathological changes and ultrastructural changes of Hetian sheep were observed by replicating the subchronic poisoning model of Hetian sheep. The expression of reproductive gene mRNA was detected by Real-Time Q PCR method and the protein expression was detected by Western Blot. The results showed that the ovarian index was significantly increased and the number of follicles on the surface of the ovary decreased significantly. The microscopic examination showed that the primary oocytes dissolved and disappeared, and the interstitial blood vessels dilated. Tunel showed that the apoptosis of ovarian cells and follicular granulosa cells was observed, and the expression of Bax gene mRNA was significantly increased. The expression of Bcl-2 gene mRNA was significantly decreased (P 0.01). The activities of superoxide dismutase (SODX), glutathione peroxidase (GSH-Px) and catalase (catalase) in serum and ovary tissues were significantly decreased (P 0.01), and the contents of malondialdehyde (MDAs) were significantly increased in serum, estradiol (E 2) and catalase (CAT) in the serum and ovary tissues of the sheep. The content of P _ (4) in progesterone decreased significantly (P _ (0.01)). The expression of FSHR and LHR mRNA in the ovary was significantly decreased, and the protein expression was significantly decreased. The results showed that the toxicity of small flower ratchet bean could induce ovarian pathological damage, inhibit the expression of related reproductive genes, promote cell apoptosis, and thus inhibit the reproductive function of Hetian sheep.
【作者單位】： 塔里木大學(xué)動(dòng)物科學(xué)學(xué)院/新疆生產(chǎn)建設(shè)兵團(tuán)塔里木畜牧科技重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31460678) 國家星火計(jì)劃項(xiàng)目(2015GA891015)~~
【分類號(hào)】：S858.26

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 斯拉吉丁;對(duì)和田羊改良及有關(guān)問題的意見[J];新疆畜牧業(yè);2000年04期

2 於建國;董紅;季躍光;孔艷麗;古麗格娜;肉孜買買提;楊飛;亞森·庫爾班;溫且木;米日尼沙;;和田羊品種資源現(xiàn)狀與保護(hù)利用措施[J];草食家畜;2011年02期

3 李一善,陳玉驊;和田羊[J];新疆農(nóng)業(yè)科學(xué);1962年05期

4 道良佐,鄂璞,郭功騎,項(xiàng)毓華,惠恩廣,陳玉驊;和田羊調(diào)查報(bào)告(第一部分)[J];新疆農(nóng)業(yè)科學(xué);1963年12期

5 王錚;李小兵;;阿萊羊?qū)牒吞镅螂s交效果探討[J];新疆畜牧業(yè);2007年04期

6 李小兵;王錚;;和田羊生產(chǎn)性能變化分析[J];草食家畜;2008年04期

7 李萍;;新疆和田羊種質(zhì)資源的保護(hù)和利用現(xiàn)狀[J];當(dāng)代畜牧;2011年05期

8 道良佐,鄂璞,郭功騎,惠恩^，

本文編號(hào)：1787923