本文選題：玉米赤霉烯酮 + 斷奶小母豬　； 參考：《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2017年12期

【摘要】：本試驗(yàn)旨在研究不同水平玉米赤霉烯酮(ZEA)污染飼糧對(duì)斷奶小母豬卵巢指數(shù)及孕激素受體的陽(yáng)性分布和mRNA相對(duì)表達(dá)量的影響。選擇40頭35~38日齡健康三元雜交(杜×長(zhǎng)×大)斷奶小母豬,按平均體重[(14.01±0.86)kg]隨機(jī)分為4個(gè)組,采用單籠飼養(yǎng)。對(duì)照組飼喂基礎(chǔ)飼糧,試驗(yàn)組分別在基礎(chǔ)飼糧中添加0.5、1.0和1.5 mg/kg的ZEA,4個(gè)組ZEA的測(cè)定值分別為0、(0.52±0.07)mg/kg、(1.04±0.03)mg/kg和(1.51±0.13)mg/kg。試驗(yàn)期35 d。結(jié)果表明:隨著飼糧ZEA水平的升高,斷奶小母豬卵巢指數(shù)呈一次線性升高(P0.01);1.0 mg/kg ZEA組的卵巢指數(shù)顯著高于1.5 mg/kg ZEA組(P0.05),1.5 mg/kg ZEA組的卵巢指數(shù)顯著高于0.5 mg/kg ZEA組和對(duì)照組(P0.05)。隨著飼糧ZEA水平的升高,斷奶小母豬卵巢孕激素受體累積光密度(IOD)和mRNA相對(duì)表達(dá)量均呈一次線性升高(P0.01);1.5 mg/kg ZEA組的孕激素受體IOD和mRNA相對(duì)表達(dá)量顯著高于1.0 mg/kg ZEA組(P0.05),1.0 mg/kg ZEA組又顯著高于0.5 mg/kg ZEA組和對(duì)照組(P0.05)。免疫組化結(jié)果顯示斷奶小母豬卵巢中孕激素受體免疫陽(yáng)性物質(zhì)主要分布于原始卵泡和生長(zhǎng)卵泡的卵母細(xì)胞及顆粒細(xì)胞、卵泡膜細(xì)胞及血管壁細(xì)胞。隨著飼糧ZEA水平的升高和卵泡閉鎖程度的增加,免疫陽(yáng)性反應(yīng)明顯增強(qiáng),但卵巢內(nèi)孕激素受體免疫陽(yáng)性物質(zhì)的分布位置沒(méi)有因ZEA的水平發(fā)生明顯改變。由此可見(jiàn),ZEA(1.0~1.5 mg/kg)可以通過(guò)調(diào)控?cái)嗄绦∧肛i卵巢中孕激素受體的高水平表達(dá),使孕激素受體的水平超出正向調(diào)節(jié)卵泡生長(zhǎng)的限值,從而促使卵泡閉鎖,降低卵巢指數(shù),改變卵巢發(fā)育,進(jìn)而影響斷奶小母豬生殖系統(tǒng)的健康。
[Abstract]:The purpose of this study was to study the effects of different levels of Zea on ovarian index, progesterone receptor positive distribution and relative expression of mRNA in weaned sows. Forty 35 脳 38 days old healthy three-way crossbred (du 脳 long 脳 big) weaned sows were randomly divided into 4 groups according to their average body weight [14.01 鹵0.86)kg] and raised in a single cage. The control group was fed with basic diet, and the experimental group was fed with 0.5 mg/kg and 1.5 mg/kg, respectively. The ZEA values of the four groups were 0.52 鹵0.07 mg 路kg ~ (-1) 0.03)mg/kg and 1.51 鹵0.13 mg 路kg ~ (-1) 路kg ~ (-1) respectively. The trial period was 35 days. The results showed that with the increase of dietary ZEA level, the ovarian index of weaning sows showed a linear increase. The ovarian index of weaned sows in 1.0 mg/kg ZEA group was significantly higher than that in 1.5 mg/kg ZEA group (P0.05 mg/kg ZEA group) and in 0.5 mg/kg ZEA group and control group (P0.05). The results showed that the ovarian index of weaning sows was significantly higher than that of 1.5 mg/kg ZEA group. With the increase of dietary ZEA level, The relative expression of progesterone receptor (IOD) and progesterone receptor (mRNA) in the ovary of weaning sows were increased linearly. The relative expression of progesterone receptor IOD and mRNA in the 1.5 mg/kg ZEA group was significantly higher than that in the 1.0 mg/kg ZEA group and the 0.5 mg/kg ZEA group and the control group P0.055.The relative expression of progesterone receptor IOD and mRNA in the ovary of weaning sows was significantly higher than that in the 1.0 mg/kg ZEA group and the 0.5 mg/kg ZEA group and the control group. Immunohistochemical results showed that progesterone receptor immunoreactive substances were mainly distributed in oocytes and granulosa cells of primordial follicles and growth follicles, follicular membrane cells and vascular parietal cells. With the increase of dietary ZEA level and follicular atresia, the immunoreactive reaction was significantly enhanced, but the distribution of progesterone receptor immunoreactive substance in ovary was not significantly changed by the level of ZEA. It can be seen that ZEAA 1.0 mg / kg can regulate the high level of progesterone receptor expression in the ovaries of weaning sows, so that the level of progesterone receptor exceeds the limit of positive regulation of follicular growth, thus promoting follicular atresia and reducing ovarian index. Change the development of ovaries, and then affect the reproductive health of weaning sows.
【作者單位】： 山東農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院山東省動(dòng)物生物工程與疾病防治重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(31572441) 山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系生豬創(chuàng)新團(tuán)隊(duì)建設(shè)項(xiàng)目(SDAIT-08-04) “雙一流”獎(jiǎng)補(bǔ)資金資助
【分類(lèi)號(hào)】：S828.5

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