本文選題：超聲波 + 優(yōu)化��； 參考：《農(nóng)業(yè)工程學(xué)報(bào)》2017年13期

【摘要】：病死畜禽資源化利用是解決病死畜禽污染的一條重要途徑。為探索病死豬肉酶解工藝條件,該文以豬肉為原料,以胰蛋白酶為試驗(yàn)用酶,以水解度為指標(biāo),選取加酶量、底物濃度、pH值、溫度作為試驗(yàn)因素,通過單因素試驗(yàn)初步確定了胰蛋白酶的水解條件,并分析了各因素對酶解反應(yīng)的影響規(guī)律。應(yīng)用Box-Behnken中心組合設(shè)計(jì)建立數(shù)學(xué)模型,以水解度為響應(yīng)值,進(jìn)行了四因素三水平的響應(yīng)面優(yōu)化試驗(yàn),確定了最佳酶解工藝條件,并通過響應(yīng)面模型的曲面圖直觀地分析了各影響因素之間的交互作用。在此基礎(chǔ)上,探索頻率為20 k Hz,功率為500 W的超聲波預(yù)處理豬肉20 min對酶解效果的影響,并應(yīng)用掃描電子顯微鏡在微觀結(jié)構(gòu)上對其原因進(jìn)行了分析。結(jié)果表明,各因素對酶解反應(yīng)的影響大小依次為:加酶量溫度pH值底物濃度,在試驗(yàn)范圍內(nèi)得到的酶解最佳工藝條件為:加酶量為1.15%(質(zhì)量分?jǐn)?shù))、底物質(zhì)量濃度為80.5 g/L、pH值為7.96、溫度為40.6℃,酶解1 h的預(yù)測水解度可達(dá)16.74%,驗(yàn)證試驗(yàn)水解度為16.77%,表明試驗(yàn)結(jié)果與軟件分析結(jié)果相符,最佳水解時間為6 h,此時的水解度為28.91%。超聲波預(yù)處理后,最佳水解時間為4 h,水解度達(dá)到32.86%,因此超聲波預(yù)處理能縮短水解周期2 h,提高水解度4個百分點(diǎn)。由此可見,應(yīng)用超聲波預(yù)處理可以提高酶解效率,縮短工作時間。
[Abstract]:The resource utilization of sick and dead livestock is an important way to solve the pollution of sick and dead livestock and poultry. In order to explore the technological conditions of enzymatic hydrolysis of dead pork, this paper takes pork as raw material, trypsin as experimental enzyme, hydrolysis degree as index, enzyme dosage, substrate concentration, pH value and temperature as experimental factors. The hydrolysis conditions of trypsin were preliminarily determined by single factor test, and the influence of each factor on enzymatic hydrolysis was analyzed. The mathematical model was established by using the Box-Behnken center combination design. The optimum conditions of enzymatic hydrolysis were determined by using four factors and three levels of response surface optimization test with the degree of hydrolysis as the response value. The interaction between the factors is analyzed intuitively by the surface graph of the response surface model. On this basis, the effects of ultrasonic pretreatment of pork for 20 min at a frequency of 20kHz and 500W on the enzymatic hydrolysis were investigated, and the causes were analyzed by scanning electron microscope (SEM). The results showed that the effect of various factors on enzymatic hydrolysis was as follows: enzyme dosage, temperature, pH value, substrate concentration, The optimum conditions of enzymatic hydrolysis were obtained in the range of experiment: the amount of enzyme added was 1.15 (mass fraction), the substrate concentration was 80.5 g / L ~ (-1) pH value was 7.96, and the temperature was 40.6 鈩，

本文編號：1778188