本文選題：絲蘭提取物 + 肉仔雞��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本論文通過在日糧中添加不同濃度的絲蘭提取物(Yucca schidigera extract,YSE),研究YSE對肉仔雞抗氧化和免疫功能的影響,探討YSE在肉仔雞日糧中的應(yīng)用效果,為確定肉仔雞日糧中YSE的適宜添加量提供理論依據(jù)。試驗選取體重相近的健康15日齡的AA肉仔雞128只,隨機(jī)分為4個處理組,每個處理4個重復(fù),每個重復(fù)8只雞(公母各半)。將玉米豆粕型日糧作為基礎(chǔ)日糧,對照組飼喂基礎(chǔ)日糧,3個YSE添加組分別添加100、200、300 mg/kg的YSE。試驗期28 d,分為試驗前期(15～28 d)和試驗后期(29～42 d),各14 d。論文共分三個試驗內(nèi)容:試驗一:通過測定血清、小腸、肝臟、胸肌樣品中抗氧化酶活力及其產(chǎn)物濃度,并測定組織中抗氧化酶基因的相對表達(dá)量,研究YSE對于肉仔雞抗氧化功能的影響。.結(jié)果表明:在14 d,血清中總抗氧化能力(T-AOC)和過氧化氫酶(CAT)水平均在200 mg/kg YSE添加劑量時提高(P=0.031,P=0.009),血清中超氧化物歧化酶(SOD)活性在所有YSE添加水平均升高(P=0.014);200mg/kg的YSE添加水平提高了肝臟中CAT活力及其基因表達(dá)(P=0.035,P=0.003);十二指腸和回腸中T-AOC(P=0.039,P=0.031)、谷胱甘肽過氧化物酶(GPx)(P=0.016,P=0.045)和SOD(P=0.001,P=0.023)水平在100mg/kgYSE添加水平時均升高;100mg/kgYSE上調(diào)了回腸中SOD和GPx的基因表達(dá)(P=0.018,P=0.003),回腸中CAT的活力及其基因相對表達(dá)量在100 mg/kg添加水平時升高(P0.001,P=0.028)。在28 d,血清中T-AOC水平在200和300 mg/kg劑量時上升(P=0.006),但MDA含量僅在300 mg/kg時顯著降低(P=0.019);肝臟T-AOC水平在300 mg/kg YSE添加劑量時顯著提高(P=0.001);300mg/kgYSE添加量顯著升高了肝臟中GPx和SOD活性(P=0.002,P=0.049),并上調(diào)了其GPx的基因表達(dá)(P=0.028);肝臟MDA濃度在300 mg/kg劑量組顯著降低(P=0.006);十二指腸SOD活力在100mg/kg時上升(P=0.039);所有YSE添加組均能提升回腸中GPx水平(P=0.022)。300mg/kgYSE添加能夠顯著提高胸肌T-AOC的水平(P=0.017)。試驗二:通過測定血清中免疫細(xì)胞因子濃度及新城疫抗體滴度,探討在肉仔雞日糧中添加YSE對其免疫功能的影響。結(jié)果表明:在14 d,血清中白介素(IL)-6濃度在 200 和 300 mg/kg YSE 添加水平時降低(P0.001);在 28 d,100 mg/kg YSE濃度降低了 IL-4濃度(P=0.002),提高了 IL-6和γ干擾素(IFN-γ)的濃度(P=0.004,P=0.026);在14 d和28 d,新城疫抗體滴度在100 mg/kg YSE添加水平時升高(P0.001,P=0.032)。試驗三:通過測定血清中一氧化氮(NO)及誘導(dǎo)型一氧化氮合酶(iNOS)水平,及肝臟、脾臟、小腸中iNOS的基因表達(dá),從NO途徑探究YSE對于肉仔雞免疫及抗氧化功能的影響。結(jié)果表明:在14d,100mg/kgYSE促進(jìn)了血清中NO含量和iNOS的活性(P=0.032,P=0.048),200 mg/kg YSE上調(diào)了回腸中iNOS的基因表達(dá)(P=0.031),肝臟和脾臟中iNOS基因表達(dá)在100 mg/kg YSE添加水平呈現(xiàn)上調(diào)趨勢(P=0.081,P=0.075);在28d,與對照組相比,YSE處理組血清中NO和iNOS濃度,組織中iNOS的基因表達(dá)均未出現(xiàn)顯著差異。本試驗結(jié)果表明,在日糧中添加YSE能夠提升肉仔雞的抗氧化及免疫功能。100、200和300 mg/kg YSE添加水平能夠通過提高不同組織的抗氧化指標(biāo)來提升肉仔雞機(jī)體的抗氧化功能;100mg/kg的YSE添加能夠促進(jìn)肉仔雞的免疫功能;100mg/kg的YSE添加能夠通過提升血清中NO濃度及iNOS活性從而提升肉仔雞的抗氧化和免疫功能。
[Abstract]:In this paper, by adding different concentrations of yucca extract in diets (Yucca schidigera, extract, YSE), the effects of YSE on antioxidant and immune function of broilers, to explore the application effect of YSE in broiler diets, as determined in broiler diets the appropriate addition amount of YSE and provide a theoretical basis for the similar weight. 15 day old 128 AA broiler chickens were selected, randomly divided into 4 groups with 4 replicates of 8 chicks per replicate (male and female). The corn soybean meal diet as basal diet. The control group was fed basal diet, 3 YSE supplementation groups were added 100200300 mg/kg YSE. test of 28 d, divided into pre test (15~28 d) and the end of the experiment (29~42 d), each 14 d. of the experiment was divided into three parts: test one: by determination of serum, small intestine, liver, antioxidant enzyme activity and product concentration in sample and determination of muscle, tissue The relative expression of anti oxidase gene, effect of YSE on the antioxidant function in broilers. The results showed that at 14 d, the total antioxidant capacity in serum (T-AOC) and catalase (CAT) levels were increased in 200 mg/kg YSE added dose (P=0.031, P=0.009), serum superoxide dismutase (SOD) activity increased in all YSE levels (P=0.014); 200mg/kg YSE added to improve the level of CAT activity and gene expression in the liver (P=0.035, P=0.003); T-AOC in the duodenum and ileum (P=0.039, P=0.031), glutathione peroxidase (GPx) and Gu Guanggan (P=0.016, P=0.045) and SOD (P=0.001, P=0.023) level increased in 100mg/kgYSE when the addition level of 100mg/kgYSE increased; the expression of SOD and GPx in the ileum of genes (P=0.018, P=0.003), relative activity and mRNA expression in the ileum of CAT increased at 100 mg/kg when the addition level of (P0.001, P=0.028). In 28 d, blood In the T-AOC level increased in 200 and 300 of the dose of mg/kg (P=0.006), but the content of MDA in only 300 mg/kg significantly decreased (P=0.019); liver T-AOC levels increased significantly in the 300 mg/kg YSE adding dose (P=0.001); the amount of 300mg/kgYSE significantly increased GPx and SOD activity in liver (P=0.002, P=0.049). And raised the expression of GPx gene (P=0.028); liver MDA concentration decreased significantly in the 300 mg/kg group (P=0.006); the activity of SOD 100mg/kg increased in the duodenum (P=0.039); all YSE added groups can raise GPx level in the ileum (P= 0.022).300mg/kgYSE addition can significantly improve the level of T-AOC (P=0.017). Two. Test: through the determination of serum cytokine concentrations and Newcastle disease antibody titer, investigated in broiler diets the effect of YSE on immune function. The results showed that at 14 d, serum interleukin (IL) -6 concentration at 200 and 300 mg/k G YSE decreased when the addition level (P0.001); in 28 d, 100 mg/kg YSE concentration decreased the concentration of IL-4 (P=0.002), increased IL-6 and interferon gamma (IFN- gamma) concentration (P=0.004, P=0.026); in 14 d and 28 d, Newcastle disease antibody titer increased at 100 mg/kg when the addition level of YSE (P0.001, P=0.032). Experiment three: the determination of serum nitric oxide (NO) and inducible nitric oxide synthase (iNOS) levels, and the liver, spleen, intestinal iNOS gene expression, to explore the impact of YSE on immune function and antioxidation of broiler chickens from the NO pathway. The results showed that: in 14d, 100mg/kgYSE promoted NO content and iNOS activity in serum (P=0.032, P=0.048), 200 mg/kg YSE up-regulated the expression of iNOS gene in the ileum (P=0.031, iNOS) in the liver and spleen gene expression level increased in adding 100 mg/kg YSE (P=0.081, P= 0.075); in 28d, compared with the control group, YSE treatment group in serum N O and iNOS concentration, the expression of iNOS gene had no significant difference. The test results show that the addition of YSE can enhance the antioxidant and immune function of.100200 broilers and 300 mg/kg YSE levels can increase the antioxidant index of different tissues to enhance the body's antioxidant function in Broilers Diets; 100mg/kg YSE add can promote the immune function of broilers; 100mg/kg YSE is added to be able to enhance the concentrations of serum NO and iNOS activity so as to enhance the antioxidant and immune function of broilers.

【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S831.5

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本文編號：1770660