本文選題：黃芪 + 靈芝　； 參考：《揚州大學》2015年碩士論文

【摘要】：黃芪和靈芝是傳統(tǒng)的中草藥,主要活性成分為黃芪多糖和靈芝多糖,具有免疫增強作用。黃芪靈芝口服液為某生物技術公司研制的新產品,黃芪多糖和靈芝多糖為其有效成分。本文研究黃芪靈芝口服液對正常及免疫抑制模型雛雞免疫功能及ND疫苗免疫作用的影響,旨在探討黃芪靈芝口服液的免疫增強作用,為黃芪靈芝口服液作為一種新型免疫增強劑應用于獸醫(yī)臨床提供實驗依據(jù)。對雛雞免疫功能的影響研究：7日齡黃羽肉雞180只,隨機分成9組。第1-3組分別按10、5、1 mL/kg劑量灌服黃芪靈芝口服液,第4-7組分別按80 mg/kg劑量肌內注射環(huán)磷酰胺復制免疫抑制模型,第4-6組同時分別給予10、5、1 mL/kg黃芪靈芝口服液,第8-9組分別按1 mL/kg劑量灌服陽性對照物黃芪精口服液和生理鹽水,黃芪靈芝口服液、黃芪精口服液及生理鹽水連用5天,環(huán)磷酰胺連用3天。分別于12、17、22日齡從各組隨機采樣,測定免疫器官指數(shù)、脾淋巴細胞增殖及血清中IL-2水平。結果表明,黃芪靈芝口服液高、中、低劑量組在12、17、22日齡時,脾淋巴細胞增殖顯著高于生理鹽水對照組和免疫抑制模型組(P0.05)。22日齡時,黃芪靈芝口服液高、中、低劑量組IL-2水平顯著高于生理鹽水對照組和免疫抑制模型組(P0.05)。黃芪靈芝口服液中劑量組的胸腺和法氏囊指數(shù)顯著高于生理鹽水對照組和免疫抑制模型組(P0.05)。黃芪靈芝口服液灌服能促進雛雞免疫功能,在一定程度上能有效拮抗由環(huán)磷酰胺引起的免疫抑制。對雛雞ND疫苗免疫作用的影響研究：14日齡黃羽肉雞180只,隨機分成9組。1-9組通過滴眼滴鼻接種新城疫活疫苗,第1～3組分別按10、5、1 mL/kg劑量灌服黃芪靈芝口服液,第4～7組分別按80 mg/kg劑量肌內注射環(huán)磷酰胺,第4-6組同時分別給予按10、5、1 mL/kg黃芪靈芝口服液,第8組以1 mL/kg劑量灌服黃芪精口服液陽性對照物；第9組給予生理鹽水作為疫苗對照組。黃芪靈芝口服液、黃芪精口服液及生理鹽水連用5天,環(huán)磷酰胺連用3天。分別于免疫后第7、14、21、28天從各組隨機采樣,測定免疫器官指數(shù)、脾淋巴細胞增殖、血清中IL-2水平及ND抗體滴度。結果表明,黃芪靈芝口服液高、中、低劑量組脾淋巴細胞增殖在免疫后第7、14、21、28天顯著高于疫苗對照組(P0.05)；IL-2水平在免疫后3w顯著高于疫苗對照組(P0.05)；免疫后1 w,受試物所有劑量組的抗體滴度均高于疫苗對照組,且抗體滴度維持的時間與疫苗組相當,黃芪靈芝口服液能促進ND疫苗免疫雛雞后的抗體產生。綜上所述,黃芪靈芝口服液可增強雛雞免疫功能及ND疫苗的免疫作用,可望作為免疫增強劑使用。
[Abstract]:Astragalus membranaceus and Ganoderma lucidum are traditional Chinese herbal medicines. The main active components are astragalus polysaccharide and Ganoderma lucidum polysaccharide.Astragalus ganoderma oral liquid is a new product developed by a biotechnology company. Astragalus polysaccharides and Ganoderma lucidum polysaccharides are effective components.The effects of Huangqi Ganoderma lucidum oral liquid on immune function and ND vaccine immunity of normal and immunosuppressive chicks were studied in order to explore the immune enhancement effect of Huangqi Ganoderma lucidum oral liquid.To provide experimental evidence for the application of Astragalus Ganoderma oral liquid as a new immune enhancer in veterinary clinic.Study on immune function of Chicks 180 yellow broilers aged 7 days were randomly divided into 9 groups.Group 1-3 was given Astragalus Ganoderma oral liquid at a dose of 10 mL/kg, group 4-7 was injected intramuscularly with cyclophosphamide at a dose of 80 mg/kg to establish immunosuppressive model, group 4-6 was given 10 mL/kg Astragalus ganoderma oral liquid at the same time, and group 4-7 was injected intramuscularly with cyclophosphamide at a dose of 80 mg/kg.In group 8-9, Huangqi essence oral liquid and normal saline, astragalus lucidum oral liquid, astragalus essence oral liquid and normal saline were given at the dose of 1 mL/kg for 5 days, and cyclophosphamide for 3 days.The immune organ index, splenic lymphocyte proliferation and serum IL-2 level were measured by random sampling at the age of 1722 days.The results showed that the proliferation of splenic lymphocytes in Astragalus lucidum oral liquid was significantly higher than that in normal saline control group and immunosuppressive model group at the age of 12 days 1722 days, and the oral liquid of Astragalus lucidum was high and medium at 22 days of age, and the dose of Astragalus Ganoderma lucidum oral liquid was higher than that of normal saline control group and immunosuppressive model group.The level of IL-2 in low dose group was significantly higher than that in normal saline control group and immunosuppressive model group (P 0.05).The thymus and bursa index of middle dose group of Huangqi Ganoderma lucidum oral liquid was significantly higher than that of normal saline control group and immunosuppressive model group (P 0.05).Huangqi Ganoderma lucidum oral solution could promote the immune function of chicks and antagonize the immunosuppression caused by cyclophosphamide to some extent.Effects of ND Vaccine on immunity of Chicks 180 broilers aged 14 days were randomly divided into 9 groups. Group 1-9 was given live Newcastle disease vaccine by intranasal drip. The first and third groups were given Astragalus Ganoderma oral solution at the dose of 10 ~ 51g / mL/kg, respectively.In group 4, cyclophosphamide was injected intramuscularly at the dose of 80 mg/kg, group 4-6 was given Astragalus ganoderma oral liquid for 10 mL/kg, and group 8 was administered with 1 mL/kg dose of Astragalus essence oral liquid as positive control.Group 9 was given normal saline as vaccine control group.Astragalus lucidum oral liquid, astragalus essence oral liquid and normal saline were used for 5 days and cyclophosphamide for 3 days.The immune organ index, spleen lymphocyte proliferation, serum IL-2 level and ND antibody titer were measured by random sampling on day 28 after immunization.The results showed that Huangqi Ganoderma lucidum oral liquid was high and moderate.The proliferation of spleen lymphocytes in the low dose group was significantly higher than that in the vaccine control group on the 28th day after immunization, and the level of IL-2 was significantly higher than that in the vaccine control group at 3 weeks after immunization, and the titer of antibody in all dose groups was higher than that in the vaccine control group at 1 week after immunization.Astragalus lucidum oral liquid could promote antibody production after ND vaccine immunization.To sum up, Astragalus Ganoderma oral liquid can enhance the immune function of chicks and the immune effect of ND vaccine, which is expected to be used as immune enhancer.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S853.7

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本文編號：1763193