發(fā)布時間：2018-04-16 21:32

  本文選題：大通犢牦牛 + 線粒體��； 參考：《畜牧與獸醫(yī)》2017年12期

【摘要】：為探討高原低氧環(huán)境下大通犢牦牛線粒體琥珀酸脫氫酶(SDH)在不同組織中的表達模式以及SDH活性與其亞基mRNA表達量之間的關系,采用酶聯(lián)免疫分析法(ELISA)對犢牦牛心肌、骨骼肌、大腦、肝臟組織中線粒體SDH活性進行測定;實時熒光定量PCR(Real-Time PCR)檢測組織中Sdha、Sdhb、Sdhc和Sdhd亞基mRNA表達量。結果表明:大通犢牦牛大腦、心肌、骨骼肌組織中線粒體SDH活性顯著高于樂都犢牦牛;大腦組織中線粒體Sdh4個亞基mRNA表達量極顯著高于樂都犢牦牛。結論:含野血大通犢牦牛組織線粒體SDH有氧代謝功能強于樂都犢牦牛;大腦組織線粒體Sdh4個亞基轉錄水平較高;大通犢牦牛大腦組織線粒體SDH活性與Sdh4個亞基mRNA表達量呈正相關。
[Abstract]:In order to investigate the expression pattern of mitochondrial succinate dehydrogenase (SDH) in different tissues and the relationship between the activity of SDH and the expression of its subunit mRNA in Datong calf yak under high altitude hypoxia, the myocardial and skeletal muscles of calf yak were treated by Elisa.The activity of mitochondrial SDH was measured in brain and liver, and the expression of Sdhagsdhbhc and Sdhd subunit mRNA was detected by real-time fluorescence quantitative PCR(Real-Time.The results showed that the activity of mitochondrial SDH in brain, myocardium and skeletal muscle of Datong calf yak was significantly higher than that of Ledong calf yak, and the mRNA expression of mitochondrial Sdh4 subunit in brain tissue was significantly higher than that in Ledong calf yak.Conclusion: the aerobic metabolism function of mitochondrial SDH in yak with wild blood is stronger than that in Ledong calf, and the transcription level of mitochondrial Sdh4 subunit in brain tissue is higher than that in Ledong calf yak.There was a positive correlation between mitochondrial SDH activity and mRNA expression of Sdh4 subunits in the brain of Datong calf yak.
【作者單位】： 青海大學農牧學院;
【基金】：國家自然科學基金項目資助(31460643) 青海省農牧廳項目(NMSY-2016-04)
【分類號】：S823.85

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