本文選題：黃芪多糖 + PRRSV　； 參考：《安徽農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：豬繁殖與呼吸綜合征(porcine reproductive and respiratory syndrome,PRRS)又名豬藍(lán)耳病,是由豬繁殖與呼吸綜合征病毒(porcine reproductive and respiratory syndrome virus.PRRSV)引起豬以繁殖障礙和呼吸系統(tǒng)癥狀為特征的一種急性、高度傳染的病毒性傳染病。該病已成為嚴(yán)重威脅我國(guó)養(yǎng)豬業(yè)發(fā)展的重要傳染病之一。當(dāng)前疫苗免疫是防制我國(guó)豬群PRRS的重要手段。為了探究黃芪多糖促進(jìn)PRRSV疫苗免疫效果和抗PRRSV野毒感染能力,本研究開(kāi)展如下三方面的研究:1.黃芪多糖對(duì)PRRSV VR-2332株弱毒苗免疫效果的研究。本研究選取70頭后備母豬,根據(jù)其血液中PRRSV抗體水平和細(xì)胞因子(IL-2和IFN-γ)水平,平均分為兩組(試驗(yàn)組A和對(duì)照組B)。根據(jù)PRRSV抗體S/P值大小,將A組,再分為S/P值1.0以下組(A1組),S/P值1.0-2.0組(A2組),S/P值2.0以上組(A3組),B組分組方法同A。A組連續(xù)飼喂黃芪多糖14天,B組不飼喂黃芪多糖。飼喂至第8天,A、B兩組免疫PRRSV弱毒疫苗。免疫后第10天和第25天對(duì)A、B兩組母豬進(jìn)行前腔靜脈采血。利用ELISA方法測(cè)定PRRSV抗體和細(xì)胞因子(IL-2和IFN-γ)水平。分析A、B兩組PRRSV抗體和細(xì)胞因子水平的變化。結(jié)果表明,(1)A、B組PRRSV疫苗免疫效果無(wú)明顯差異。但對(duì)免疫前S/P1.0的豬群,連續(xù)飼喂黃芪多糖14天會(huì)對(duì)PRRSV疫苗免疫效果有明顯的抑制作用;(2)PRRSV疫苗免疫后,A、B組外周血中IL-2和IFN-γ均會(huì)上升,但A組IL-2和IFN-γ水平上升的更快,且IFN-γ水平上升持續(xù)時(shí)間更長(zhǎng)。研究結(jié)果提示,PRRSV疫苗免疫前后各一周飼喂黃芪多糖,對(duì)PRRSV疫苗抗體水平增長(zhǎng)沒(méi)有明顯的促進(jìn)作用,但可以有效促進(jìn)IL-2和IFN-γ的增長(zhǎng)。2.PRRSV病原檢測(cè)方法選擇本探究性試驗(yàn)以稀釋不同倍數(shù)的PRRSV弱毒活疫苗為樣本,分別使用常規(guī)RT-PCR、熒光RT-PCR及POCKIT便攜式PCR檢測(cè)系統(tǒng)對(duì)稀釋不同倍數(shù)PRRSV弱毒苗進(jìn)行PRRSV抗原檢測(cè)。結(jié)果發(fā)現(xiàn):POCKIT便攜式PCR檢測(cè)系統(tǒng)的檢測(cè)敏感度高,操作簡(jiǎn)單,使用于研究黃芪多糖對(duì)于妊娠母豬抗PRRSV試驗(yàn)PRRSV病原的檢測(cè)工作。3.黃芪多糖對(duì)妊娠母豬抗PRRSV的研究。本研究從未免疫PRRSV疫苗且存在PRRSV帶毒的豬群中選取70頭妊娠第25天的母豬,根據(jù)PRRSV抗體水平和細(xì)胞因子(IL-2和IFN-γ)水平及血常規(guī)指標(biāo),平均分為兩組(試驗(yàn)組C和對(duì)照組D)。C組連續(xù)飼喂黃芪多糖60天,D組不飼喂黃芪多糖。在飼喂黃芪多糖第30天和第60天分別采集C、D組前腔靜脈血,檢測(cè)PRRSV抗體、IL-2、IFN-γ和血常規(guī)值。在分娩時(shí)采集臍帶血和檢測(cè)PRRSV及統(tǒng)計(jì)繁殖指標(biāo)。結(jié)果表明,(1)飼喂黃芪多糖第30天時(shí)(即妊娠第55天),C、D兩組PRRSV抗體水平無(wú)顯著差異。飼喂黃芪多糖第60天(即妊娠第85天),C組PRRSV抗體水平顯著高于D組;(2)比較臍帶血PRRSV檢測(cè)率,C組為0%(0/35),D組為17%(6/35);(3)相比于妊娠25天,妊娠55天時(shí),C、D兩組豬群外周血中IL-2和IFN-γ含量顯著性升高,妊娠85天時(shí),C、D兩組豬群外周血中IFN-γ含量依然保持高水平。
[Abstract]:Porcine reproductive and respiratory syndrome (PRRSs), also known as porcine blue ear disease, is an acute and highly contagious viral infection characterized by reproductive disorders and respiratory symptoms in pigs caused by porcine reproductive and respiratory syndrome virus.The disease has become a serious threat to the development of pig industry in China one of the important infectious diseases.At present, vaccine immunization is an important method to prevent and control PRRS in swine herd in China.In order to investigate the effect of astragalus polysaccharide on PRRSV vaccine immunity and the ability of resisting PRRSV wild virus infection, this study carried out the following three aspects: 1.Study on the immune effect of Astragalus Polysaccharide on PRRSV VR-2332 attenuated Vaccine.According to the levels of PRRSV antibody and cytokines IL-2 and IFN- 緯 in blood, 70 sows were divided into two groups: experimental group A and control group.According to the S- P value of PRRSV antibody, group A was divided into two groups: group A (< 1.0) and group A1 (P / P = 1.0-2.0), group A _ 2: group A _ 2, group A _ 3 and group A _ 3 were treated with Astragalus polysaccharide for 14 days and group A were not fed with Astragalus polysaccharide for 14 days.Two groups of PRRSV attenuated vaccine were immunized until the 8th day.On the 10th and 25th day after immunization, the blood samples were collected from the anterior vena cava of the two groups of sows.The levels of PRRSV antibody and cytokine IL-2 and IFN- 緯 were measured by ELISA method.The changes of PRRSV antibody and cytokine levels in two groups were analyzed.The results showed that there was no significant difference in the immune effect of PRRSV vaccine in group A and B.However, the immune effect of Astragalus Polysaccharide on PRRSV vaccine was significantly inhibited by Astragalus Polysaccharide for 14 days before immunization. The levels of IL-2 and IFN- 緯 in peripheral blood of group A were higher than those of group A, but the levels of IL-2 and IFN- 緯 in group A increased more rapidly.The level of IFN- 緯 increased for a longer time.The results showed that Astragalus polysaccharide was fed with Astragalus membranaceus polysaccharide for one week before and after immunization with PRRSv vaccine, and the antibody level of PRRSV vaccine did not increase significantly.However, it can effectively promote the growth of IL-2 and IFN- 緯. 2. PRRSv pathogen detection method selected this inquiry test to dilute different times of PRRSV attenuated live vaccine as a sample,Routine RT-PCR, fluorescent RT-PCR and POCKIT portable PCR system were used to detect the PRRSV antigen of diluted different times PRRSV attenuated vaccine.The results showed that the portable PCR detection system was highly sensitive and easy to operate. It was used to study the detection of Astragalus polysaccharides against PRRSV in pregnant sows.Study on the effect of Astragalus Polysaccharide on PRRSV in pregnant sows.In this study, 70 sows on the 25th day of gestation were selected from a group of pigs that had never been immunized with PRRSV vaccine and were infected with PRRSV virus. According to the level of PRRSV antibody, the levels of cytokines IL-2 and IFN- 緯, and the blood routine indexes,The rats were divided into two groups: experimental group C and control group DX. C were fed with Astragalus polysaccharide for 60 days and group D was not fed with Astragalus polysaccharide.The blood samples of anterior vena cava of group C were collected on the 30th and 60th day after feeding with Astragalus membranaceus polysaccharides. The serum levels of IL-2mIFN- 緯 and blood routine were detected.Umbilical cord blood was collected during delivery, PRRSV was detected and reproductive index was counted.The results showed that there was no significant difference in the level of PRRSV antibody between the two groups on the 30th day (that is, the 55th day of pregnancy) fed with Astragalus polysaccharides.The level of PRRSV antibody in group C was significantly higher than that in group D on day 60 (that is, 85 days after pregnancy). The PRRSV detection rate in group C was higher than that in group C, and the rate of detection of PRRSV in group C was higher than that in group D (group D was 17.6 / 35) compared with that in group D on the 25th day of gestation, compared with that of group D, the level of PRRSV antibody in group C was significantly higher than that in group D on day 85 of pregnancy.The levels of IL-2 and IFN- 緯 in peripheral blood of two groups were significantly increased at day 55, and the levels of IFN- 緯 in peripheral blood of two groups were still high at day 85 of gestation.
【學(xué)位授予單位】：安徽農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.28

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