本文選題：廣東地區(qū) + 豬流感病毒�。� 參考：《中國獸醫(yī)學報》2017年02期

【摘要】：為了解廣東地區(qū)豬流感病毒(SIV)的流行和變異情況,本研究于2013年10月至2014年1月從廣東省4個不同地區(qū)采集豬鼻拭子和肺臟病料共203份,對樣品處理之后進行雞胚分離和RT-PCR檢測,從中分離得到5株SIV,對其進行病毒純化、全基因組測序和遺傳進化分析。結果表明,5株SIV分離毒株其中3株為H1N1亞型,2株為H1N2。分離毒株HA裂解位點位于PSIQSR↓GL,具有典型的低致病性流感病毒特征。HA基因序列比對結果顯示,5株SIV分離毒株與A/Jiangsu/ALS1/2011(H1N1)株同源性最高,核苷酸相似性為97%~99%。遺傳進化分析結果顯示,5株分離毒株的PB2、PB1、PA和NP基因片段屬于PDM/09分支,M基因片段以及外部基因片段HA屬于歐亞類禽分支,NS基因片段屬于北美三元重組分支,3株H1N1亞型的NA基因屬于歐亞類禽分支,2株H1N2亞型的NA基因屬于人季節(jié)性流感分支�？乖稽c分析結果表明,分離毒株的抗原位點基本保守,但YJ28分離株HA1蛋白上發(fā)現3個氨基酸突變,分別是L69S、S137P、E222G。本研究通過對廣東省不同地區(qū)分離到的SIV進行鑒定分析,掌握SIV的變異趨勢,為豬流感的防控提供切實有效的理論依據。
[Abstract]:In order to understand the prevalence and variation of swine influenza virus (SIV) in Guangdong, 203 pig nasal swabs and lung samples were collected from 4 different areas of Guangdong province from October 2013 to January 2014.Five strains were isolated from chicken embryo and detected by RT-PCR after sample treatment. The virus was purified, the whole genome was sequenced and the genetic evolution was analyzed.The results showed that 5 SIV isolates were isolated, of which 3 were H1N1 subtype and 2 were H1 N 2.The HA cleavage site of the isolated strain was located at PSIQSR GLL. The results of sequence alignment of HA gene with typical low-pathogenic influenza virus showed that five isolated strains of SIV had the highest homology with A / Jiangsu-r / ALS / 2011H1 H1 / N1 strain, and the nucleotide similarity was 97 / 9999.The results of genetic evolution analysis showed that the PB2P1PA and NP gene fragments of five isolates belong to the PDM/09 branch M gene fragment and the external gene fragment HA belongs to the Eurasian avian branch NS gene fragment belonging to the H1N1 subdivision of the three North American recombinant strains.The na gene of type A belongs to the subtype of H1N2 of Eurasian avian branch 2 strains, and the na gene belongs to the human seasonal influenza branch.The results of antigenic locus analysis showed that the antigenic sites of the isolates were basically conserved, but three amino acid mutations were found on the HA1 protein of the YJ28 isolate, which were L69SX S137PNE222G.In this study, SIV isolated from different regions of Guangdong Province were identified and analyzed, and the variation trend of SIV was grasped, which provided practical and effective theoretical basis for prevention and control of swine flu.
【作者單位】： 華南農業(yè)大學獸醫(yī)學院國家生豬種業(yè)工程技術研究中心;甘肅農業(yè)大學動物醫(yī)學院;
【基金】：現代農業(yè)產業(yè)技術體系資助項目(CARS-36)
【分類號】：S852.651

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