本文選題：濱湖水牛 + 卵泡��； 參考：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：為了提高濱湖水牛繁殖力,本研究運用同期發(fā)情技術(shù)和B超診斷分析了濱湖水牛卵泡發(fā)育動態(tài)變化和生殖激素含量變化。1 湖南濱湖水牛卵泡發(fā)育動態(tài)變化的研究本研究采用同期發(fā)情技術(shù)處理濱湖水牛,利用B超檢測濱湖水牛卵泡動態(tài)發(fā)育情況。研究結(jié)果表明：(1)卵泡波出現(xiàn)時間：2波周期青年水牛的第1波和第2波分別在1.5d,8.2d出現(xiàn)；經(jīng)產(chǎn)水牛則在1.0d,12.3d出現(xiàn),兩者差異不顯著(P0.05)。3波周期中第1波、第2波、第3波分別出現(xiàn)在1.0d,7.5d和15.0d。(2)卵泡波持續(xù)時間：2波周期中,青年牛第1波、第2波分別持續(xù)7.16d、10.33d；經(jīng)產(chǎn)牛第1波、第2波分別為9.33d和11.13d。3波周期中3個波各持續(xù)6.5、8.0和9.0d。2波或3波周期,各波持續(xù)的天數(shù)間均無顯著性差異(P0.05)。(3)發(fā)情周期內(nèi)征集的卵泡總數(shù)：經(jīng)產(chǎn)牛為16.46個,要顯著高于青年牛的12.48個(P0.05)。(4)周期內(nèi)選擇的卵泡總數(shù)：經(jīng)產(chǎn)牛為7.96個,與青年牛的7.30個差異不顯著(P0.05)。(5)優(yōu)勢卵泡最大直徑：2波周期中青年牛第1、2波優(yōu)勢卵泡最大直徑分別為8.22mm和9.81mm,經(jīng)產(chǎn)牛第1、2波優(yōu)勢卵泡最大直徑分別為7.85mm和7.54mm,兩者差異不顯著(P0.05)。3波周期中的濱湖青年母水牛的第1、2、3波優(yōu)勢卵泡最大直徑分別為7.54mm、10.33mm和8.32mm。(6)各級卵泡數(shù)：青年水牛、經(jīng)產(chǎn)水牛之間在左、右卵巢上各類型卵泡的數(shù)量統(tǒng)計均無明顯差異(P0.05)。2濱湖水牛生殖激素變化的研究本研究運用放射免疫技術(shù)分析了發(fā)情周期內(nèi)生殖激素變化。研究結(jié)果表明：(1)從發(fā)情前2d到出現(xiàn)發(fā)情,FSH持續(xù)升高,發(fā)情后1d出現(xiàn)最高峰(3.5mIU/mL),然后持續(xù)回落。按發(fā)情周期各階段分析,發(fā)情中期FSH水平值最高,且顯著高于發(fā)情前期、發(fā)情后期和間情期(P0.05)；(2)從發(fā)情前期到發(fā)情第3d,LH水平呈現(xiàn)一個緩慢上升的過程,其中第3d達(dá)到22.13mIU/mL,第6d降到16.12mIU/mL;然后先緩慢升高再下降,第10d達(dá)到最低值15.17mIU/mL;之后緩慢升高,分別在14d、16d出現(xiàn)兩個小高峰,峰值分別為20.71和22.17mIU/mL,第18d出現(xiàn)最高值23.24mIU/mL。發(fā)情期三個階段(前期、中期和后期)的LH水平分別為20.65、20.02和21.29mIU/mL,但差異不顯著(P0.05),但都要顯著高于間情期的17.24mIU/mL(Pp0.05)。(3)發(fā)情前2d,E2水平最低(20.43 pg/mL),然后持續(xù)緩慢升高,發(fā)情第6d達(dá)到一個小高峰(72.88 pg/mL);之后緩慢下降,第15d到達(dá)低值(39.90 pg/mL);之后迅速上升,第19d達(dá)到最大峰值(118.32 pg/mL),隨后又迅速下降到40pg/mL以下。發(fā)情前期E2水平(67.83 pg/mL)顯著高于發(fā)清中期(51.01 pg/mL)、發(fā)情后期(56.33pg/mL)和間情期(53.74 pg/mL) (P0.05)。而其他各階段的E2水平差異不顯著(Pp0.05)。(4)發(fā)情前2d,P_4水平急劇下降,發(fā)情當(dāng)天(0d)降到最低值(0.37pg/mL),然后開始上升(出現(xiàn)三個峰值,即第7d為6.79pg/mL,10d達(dá)到最大值8.96pg/mL,14d為7.77pg/mL);隨后迅速下降。發(fā)情中期(1.87pg/mL)和發(fā)情后期(2.62pg/mL) P_4水平最低,兩者差異不顯著和(P0.05),但均低于發(fā)情前期(4.13pg/mL和間情期(5.71pg/mL),且差異顯著(P0.05),間情期P_4水平高于發(fā)情前期,且差異顯著(P0.05)。
[Abstract]:In order to improve Binhu buffalo Binhu buffalo fecundity, analysis on follicular dynamics and changes of reproductive hormones in Hunan Binhu buffalo.1 dynamic changes of follicular development by this study of estrus synchronization treatment Binhu water cow estrus synchronization and ultrasound diagnosis by this study, the use of ultrasound in Binhu buffalo follicular dynamics development. The results show that: (1) follicular wave time: first wave and second wave 2 wave period youth buffalo respectively in 1.5D, 8.2d; Buffalo multiparous appeared in 1.0d, 12.3d, had no significant difference (P0.05).3 wave period in the first wave, the second wave, the third wave were found in 1.0d, 7.5d and 15.0d. (2) follicular wave duration: 2 wave cycle, the first wave of the second wave of young cattle, respectively for 7.16d, 10.33d; multiparous cattle first waves, second waves are respectively 9.33d and 11.13d.3 in the 3 wave of the wave period of persistent 6.5,8.0 and 9.0d.2 wave Or 3 wave period, there were no significant differences in the number of days between continuous wave (P0.05). (3) the total number of follicles in the estrous period for 16.46 multiparous: cattle, was significantly higher than that of the 12.48 young cattle (P0.05). (4) the total number of follicular period selection: 7.96 multiparous cows 7.30, with no significant difference between the young cattle (P0.05). (5) the maximum diameter of the dominant follicle: 2 wave period in young cattle 1,2 wave maximum dominant follicle diameter were 8.22mm and 9.81mm, the 1,2 wave of the dominant follicle multiparous cows maximum diameter were 7.85mm and 7.54MM, the difference was not significant (P0.05) in Binhu the young mother of buffalo.3 in the period of wave 1,2,3 wave maximum diameter of dominant follicles were 7.54MM, 10.33mm and 8.32mm. (6): the number of follicles of young buffalo, buffalo in between the left and right ovarian follicle number statistics of various types were not significantly different (P0.05).2 change of reproductive hormone in Binhu Buffalo This study used radioimmunoassay analyses the change of reproductive hormones during the estrous cycle. The results show that: (1) from 2D to appear before estrus estrus, FSH continues to rise, the peak estrus after 1D (3.5mIU/mL), and then continued to fall. According to the analysis of each stage of the estrous cycle, the highest level of estrus at metaphase FSH, and was significantly higher than that of proestrus, estrus and diestrus stage (P0.05); (2) from proestrus estrus to 3D, LH levels showed a slow rise in the process, the 3D reached 22.13mIU/mL, 6D to 16.12mIU/mL; and then slowly increased and then decreased, the 10d reached the lowest value after 15.17mIU/mL; increased slowly, respectively in 14d, 16d two small peaks, the peak is 20.71 and 22.17mIU/mL respectively, the 18D peak 23.24mIU/mL. estrus three stages (early, middle and late) LH levels were respectively 20.65,20.02 and 21.29mIU/mL, but the difference 涓嶆樉钁，

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