本文選題：銅 + 凹凸棒石粘土�。� 參考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年06期

【摘要】：目的:研究開發(fā)一種抗生素替代品,應(yīng)用于動(dòng)物細(xì)菌性腹瀉病的治療。創(chuàng)新點(diǎn):將凹凸棒石的吸附作用、腸道粘膜保護(hù)作用和銅離子的殺菌作用巧妙的結(jié)合在一起,能夠替代抗生素,在動(dòng)物細(xì)菌性腹瀉的預(yù)防和治療中發(fā)揮作用。方法:用硝酸銅和凹凸棒石粘土(Pal)制備銅離子改性的凹凸棒石粘土(CM-Pal)。通過比較添加CM-Pal前后沙門氏菌數(shù)量的變化檢測抗菌活性。體內(nèi)試驗(yàn),將70只小鼠分為7組,其中5組感染沙門氏菌后分別給予Pal、CM-Pal、蒙脫石散、慶大霉素和生理鹽水;1組在感染沙門氏菌之前給予CM-Pal,作為預(yù)防組;1組不感染沙門氏菌,作為未感染組。通過糞便形態(tài)、糞便計(jì)分、生長性能和腸絨毛高度等指標(biāo)判斷對(duì)沙門氏菌引起的小鼠腹瀉的預(yù)防和治療效果。結(jié)論:體外抑菌試驗(yàn)顯示,CM-Pal與沙門氏菌作用2 h后抗菌率達(dá)100%。體內(nèi)試驗(yàn)也顯示,CM-Pal組小鼠24 h后腹瀉癥狀消失,所有小鼠恢復(fù)正常。但是其他組小鼠(Pal組、蒙脫石散組和慶大霉素組)分別在48、48和96 h后才恢復(fù)正常。CM-Pal治療組小鼠腸絨毛的高度在感染3 d后恢復(fù)正常,但是其他組小鼠腸絨毛高度恢復(fù)正常至少需要5 d。CM-Pal預(yù)防組小鼠增重高于其他組小鼠。本研究表明,CM-Pal在預(yù)防和治療由沙門氏菌引起的腹瀉方面能夠替代抗生素具有較好的效果。
[Abstract]:Objective: to research and develop a kind of substitute for antibiotics used to treat animal bacterial diarrhea. Innovations: the adsorption of attapulgite, intestinal mucosal protective effect and bactericidal effect of copper ions are combined together, to replace the antibiotics, play a role in the prevention and treatment of animal bacterial diarrhea. Methods: using copper nitrate and attapulgite (Pal) preparation of copper ion modified palygorskite clay (CM-Pal). By comparing the changes before and after the addition of CM-Pal the number of Salmonella detection of antimicrobial activity. In vivo experiment, 70 mice were divided into 7 groups, the 5 groups were treated with Pal after infection of Salmonella, CM-Pal, Montmorillonite powder, gentamicin and saline; the 1 group was given CM-Pal before infection of Salmonella, as prevention group; the 1 groups are not infected with Salmonella, as uninfected group. Through fecal morphology, fecal score, growth performance and intestinal cashmere To determine the effect of prevention and treatment of diarrhea of mice caused by Salmonella gross height index. Conclusion: the in vitro antibacterial test, the effect of CM-Pal and Salmonella 2 h antibacterial rate of 100%. in vivo experiments also showed that CM-Pal mice 24 h after diarrhea symptoms disappeared, all mice return to normal. But the other group of mice (Pal group, Montmorillonite powder group and gentamicin group) respectively at 48,48 and 96 h after.CM-Pal treatment group returned to normal mice intestinal villus height after infection 3 D returned to normal, but the other group of mice intestinal villus height recovered at least 5 d.CM-Pal weight gain prevention groups were higher than that in other groups of mice. This study showed that CM-Pal has a good effect on prevention and treatment of diarrhea can be caused by Salmonella instead of antibiotics.

【作者單位】： College
【基金】：supported by the Foundation of Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province(No.HPK201506) the Natural Science Foundation of Jiangsu Province(No.BK20130686) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China
【分類號(hào)】：S856.4

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