本文選題：斷奶仔豬 + 淀粉消化率�。� 參考：《武漢輕工大學(xué)》2015年碩士論文

【摘要】：為了測(cè)定斷奶仔豬日糧外源代謝葡萄糖,本研究進(jìn)行了四個(gè)試驗(yàn)。在比較淀粉測(cè)定方法的準(zhǔn)確性的基礎(chǔ)上,進(jìn)一步研究四種日糧養(yǎng)分的淀粉消化和利用規(guī)律。試驗(yàn)一本試驗(yàn)旨在比較三種淀粉測(cè)定方法的準(zhǔn)確性。配制6種不同比例的淀粉與纖維素組成的混合標(biāo)準(zhǔn)品,分別采用酸解法(GB/T 2008)、蒽酮比色法和酶解法測(cè)定混合標(biāo)準(zhǔn)品中淀粉含量。試驗(yàn)結(jié)果表明,酶解法測(cè)定結(jié)果比酸解法和蒽酮比色法更準(zhǔn)確,可以認(rèn)為是飼料中淀粉含量測(cè)定的最佳方法。試驗(yàn)二選取6頭21日齡杜長×大三元雜交斷奶仔豬,試驗(yàn)豬平均體重為5.48±0.61kg。研究在基礎(chǔ)日糧條件下,仔豬小腸淀粉消化和利用規(guī)律,為進(jìn)一步研究仔豬葡萄糖營養(yǎng),提供基礎(chǔ)數(shù)據(jù)。試驗(yàn)結(jié)果表明,基礎(chǔ)日糧外源代謝葡萄糖含量為472.78g/kg,在日均采食量為284.48g時(shí),試驗(yàn)斷奶仔豬每日外源代謝葡萄糖攝入量為108.09g。試驗(yàn)三選取20頭25±2日齡杜×長×大三元雜交斷奶仔豬,平均體重為7.06±0.09kg,在試驗(yàn)豬回腸末端安裝“T”型瘺管。以玉米、飼料級(jí)葡萄糖粉作為碳水化合物來源,配合4種等能等氮試驗(yàn)日糧,MG含量不同的4種試驗(yàn)日糧,分別用于消化試驗(yàn)。研究四種日糧養(yǎng)分的淀粉消化和利用規(guī)律,并根據(jù)前期試驗(yàn)結(jié)果估算四種日糧的MG值。試驗(yàn)結(jié)果表明,日糧I、II、III、IV外源代謝葡萄糖含量為254.52g/kg、315.04g/kg、451.21g/kg、490.27g/kg。在日采食量均為300g時(shí),組I、II、III、IV斷奶仔豬每日外源代謝葡萄糖攝入量分別為68.83 g、85.40 g、128.8 g、139.99g。試驗(yàn)四采本試驗(yàn)以小腸消化時(shí)間為處理因素采用單因子設(shè)計(jì),分為1h,2h,4h,6h,8h共5個(gè)處理,以試驗(yàn)三中的四種日糧為底物,建立測(cè)定外源代謝葡萄糖的體外法,并應(yīng)用該方法測(cè)定六種仔豬常用飼料外源代謝葡萄糖的含量,以為建立以仔豬代謝葡萄糖為基礎(chǔ)的飼料評(píng)定新體系和葡萄糖營養(yǎng)調(diào)控技術(shù)的實(shí)用化奠定基礎(chǔ)。試驗(yàn)結(jié)果表明:(1)體外法測(cè)定淀粉消化率的最佳消化時(shí)間為6h。(2)體內(nèi)法測(cè)得的淀粉消化率與體外消化6h的淀粉降解率的回歸方程為y=0.1374x+84.834(R2=0.8711)。由此,得到體外法測(cè)定飼料外源代謝葡萄糖的估算公式為IDSG=(0.14K'+84.83)/0.9×BS。(3)體外法測(cè)得六種仔豬常用日糧的外源代謝的含量為玉米(新疆)741.10g/kg、玉米(吉林)667.41g/kg、二等小麥(荊門)632.22g/kg、麥麩(周口)396.40 g/kg、豆粕(46%,江蘇)93.25g/kg、豆粕(43%,武漢)84.11g/kg。
[Abstract]:In order to determine exogenous glucose metabolism in weaning piglets, four experiments were conducted.On the basis of comparing the accuracy of starch determination methods, the rules of starch digestion and utilization of four kinds of dietary nutrients were further studied.The purpose of experiment one is to compare the accuracy of three starch determination methods.Six kinds of mixed standard materials composed of starch and cellulose were prepared, and the starch content in the mixed standard was determined by acidolysis GBP / T 2008, anthrone colorimetry and enzymatic hydrolysis respectively.The results showed that the results of enzymatic hydrolysis were more accurate than those of acid hydrolysis and anthrone colorimetry, which could be considered as the best method for the determination of starch content in feed.In the second experiment, 6 21 day old Ducang 脳 big three-way crossbred weaned piglets were selected. The average body weight of the tested pigs was 5.48 鹵0.61kg.In order to provide basic data for further study of glucose nutrition in piglets, the digestion and utilization of starch in small intestine of piglets were studied under the condition of basal diet.The results showed that the exogenous glucose content in basal diet was 472.78 g / kg, and the daily intake of exogenous glucose was 108.09 g when the daily intake was 284.48 g.In the third experiment, 20 25 鹵2 days old Duroc 脳 long 脳 big three-way crossbred weaned piglets with an average body weight of 7.06 鹵0.09 kg were selected to install "T" fistula at the end of the ileum of the tested pigs.Corn and fodder grade glucose powder were used as carbohydrate source and four experimental diets with different content of MG were used in digestion experiment.The starch digestion and utilization of four diets were studied, and the MG values of the four diets were estimated according to the results of previous experiments.The results showed that the exogenous metabolic glucose content was 254.52 g / kg ~ 315.04 g / kg ~ (-1) ~ 451.21 g / kg ~ (49) 0.27 g 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1).When the daily intake was 300g, the intake of exogenous glucose was 68.83 g / d, 85.40 g / d and 128.8 g / L ~ 139.99 g, respectively.In the fourth experiment, the digestion time of the small intestine was used as the treatment factor. The experiment was divided into 5 treatments of 1 h ~ 2h ~ 4h ~ 4h ~ 6h ~ (-1) ~ 8h, and the four diets of experiment 3 were used as substrates to establish an in vitro method for the determination of exogenous glucose metabolism.The method was used to determine the content of exogenous glucose in six kinds of piglets, which laid a foundation for the establishment of a new feed evaluation system based on glucose metabolism in piglets and the practical application of glucose nutrition regulation techniques.The results showed that the best digestion time of starch digestibility in vitro was 6 h. 2) the regression equation of starch digestibility measured by in vivo method and that of in vitro digestibility of starch digestibility at 6 h was YYC 0.1374x 84.834C R2O 0.8711.As a result,There are 396.40 g / kg of soybean meal, 46g of soybean meal, 93.25 g / kg of Jiangsu, 43% of soybean meal, 84.11 g / kg of Wuhan.
【學(xué)位授予單位】：武漢輕工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S828

【相似文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 方勇;斷奶仔豬外源代謝葡萄糖的評(píng)定[D];武漢輕工大學(xué);2015年

，

本文編號(hào)：1743317