本文選題：乳房炎 + 中藥混懸液�。� 參考：《黑龍江八一農(nóng)墾大學(xué)》2015年碩士論文

【摘要】：甘草總黃酮混懸乳房注射劑是由吉林大學(xué)藥理實(shí)驗(yàn)室自主研發(fā)的一種用來治療奶牛乳房炎的純中藥制劑，并經(jīng)全面質(zhì)量考察表明該制劑符合甘草總黃酮混懸液的質(zhì)量標(biāo)準(zhǔn)草案。本研究旨在證明甘草總黃酮混懸乳房注射劑臨床用藥安全，并且能夠有效治療奶牛臨床型乳房炎。 本研究主要內(nèi)容包括：通過試管二倍稀釋法觀察中藥混懸液對乳房炎主要致病菌體外抑菌效果；通過家兔熱原試驗(yàn)、家兔皮膚過敏性試驗(yàn)及肌肉刺激性試驗(yàn)、溶血與凝集檢查、健康奶牛乳房刺激性試驗(yàn)、小鼠急性毒性試驗(yàn)，觀察甘草總黃酮混懸乳房注射劑臨床用藥安全性；通過對選取的80頭患有臨床型乳房炎奶牛，，按中藥低、中、高劑量組，抗生素對照組進(jìn)行1d一次，連用5d的治療方式，然后對各組治愈率、有效率進(jìn)行對比來確定混懸液臨床用藥劑量；通過采用中藥臨床確定劑量進(jìn)行臨床治療擴(kuò)大試驗(yàn)，對60頭臨床型乳房炎奶牛進(jìn)行治療，以抗生素組、健康組為對照，對治愈率、有效率進(jìn)行統(tǒng)計(jì)，并對治療前后乳汁SCC數(shù)、泌乳量，乳清中NAGase（N-乙�；�-β-D-氨基葡萄糖苷酶）、SA（乳清白蛋白）、LDH（乳酸脫氫酶）這些指標(biāo)的升降趨勢，以及血清及乳清中SOD（超氧化物歧化酶）、GSH-PX（谷胱甘肽過氧化物酶）、MDA（丙二醛）、NO（一氧化氮）給藥前后這些指標(biāo)的活性變化進(jìn)行檢測，全面了解中藥混懸液的藥效。 體外藥敏研究說明：甘草總黃酮混懸液對無乳鏈球菌的最低抑菌濃度（MIC）、最低殺菌濃度（MBC）為4.88μg／mL，對金黃色葡萄球菌的MIC、MBC為19.53μg／mL，對大腸桿菌的MIC為625.00μg／mL。 安全性試驗(yàn)結(jié)果表明：該中藥制劑熱原性符合藥典規(guī)定；該中藥注射劑無溶血性；不會(huì)引起皮膚過敏反應(yīng)，是弱致敏物；對家兔腿部肌肉刺激級數(shù)小于2，可用于肌肉注射；對小鼠急性毒性屬于基本無毒物質(zhì)；對健康奶牛乳房刺激效果與注射用生理鹽水無差異（P0.05），以上結(jié)果證明甘草總黃酮混懸乳房注射劑符合臨床用藥安全性。 用藥劑量篩選試驗(yàn)結(jié)果表明：中藥治療組中，20ml/乳區(qū)組、40ml/乳區(qū)組、抗生素組治愈率能高達(dá)86.00%、89.36%、86.54%，并且三組之間治愈率差異性均不顯著（P＞0.05），有效率高達(dá)90.00%、93.62%、94.23%，差異不顯著（P＞0.05）；治療后發(fā)現(xiàn)由金黃色葡萄球菌、無乳鏈球菌及大腸桿菌和混合菌引發(fā)的患病乳區(qū)在中藥治療后轉(zhuǎn)陰率分別是70.00%、100.00%、28.57%、50.00%，病原菌總轉(zhuǎn)陰率高達(dá)69.04%，與抗生素組相比差異性不顯著（P＞0.05）。 臨床療效擴(kuò)大試驗(yàn)結(jié)果表明：中藥組和抗生素組治療60頭患病奶牛，治愈率分別為87.17%、86.84%，有效率高達(dá)97.44%、93.42%；在最后一次給藥后，與健康奶牛乳汁體細(xì)胞數(shù)相比，中藥組與抗生素組體細(xì)胞均恢復(fù)到正常范圍SCC＜50萬，但是抗生素組仍顯著高于健康值（P＜0.05）；患病奶牛經(jīng)過5d治療后，中藥組泌乳量恢復(fù)到正常值（P0.05）;患病奶牛治愈后乳清及血清中谷胱甘肽過氧化物酶、超氧化物歧化酶的活性明顯恢復(fù)（P＜0.05），一氧化氮、丙二醛含量明顯降低（P＜0.05或P0.01），與正常值相比無差異，乳清中乳清白蛋白、乳酸脫氫酶、N-乙酰基-β-D-氨基葡萄糖苷酶含量顯著降低（P＜0.05或P0.01）。 以上研究結(jié)果表明，甘草總黃酮混懸乳房注射劑臨床用藥安全，能夠有效修復(fù)受損的乳腺組織，治療奶牛臨床型乳房炎效果良好，為新獸藥的研發(fā)提供可靠的實(shí)踐及理論依據(jù)。
[Abstract]:Total flavonoids of Glycyrrhiza suspension injection is used in treating breast mastitis of pure Chinese medicine by the Jilin University Laboratory of pharmacology of independent research and development, and the comprehensive quality inspection showed that the quality standard draft of the preparation with total flavonoids of Glycyrrhiza suspension. The purpose of this study is to prove the TFG suspension injection breast clinical medication safety, and can effective treatment of clinical mastitis.
The main contents of this research include: the suspension of the main pathogenic bacteria of mastitis in vitro antibacterial effect through the tube two times dilution method to observe the traditional Chinese medicine; through the pyrogen test, allergic test and muscle of rabbit skin irritation test, hemolysis and agglutination test, healthy cow breast irritation test, acute toxicity test of mice, observe the total flavonoids of Glycyrrhiza mixture suspension injection breast clinical drug safety; through the 80 head selection with clinical mastitis, according to traditional Chinese medicine low, high dose group, antibiotic group 1D once and for 5D treatment, and the cure rate of each group, the efficiency were compared to determine the suspension through clinical dosage; the use of Chinese medicine to determine the clinical dose for clinical treatment of expanded testing, treatment of 60 clinical mastitis, antibiotic group, normal control group, the cure rate and effective rate. For statistics, before and after treatment SCC number of lactation, milk, whey (NAGase N- acetyl beta -D- Glucosaminidase (SA), whey albumin), LDH (lactate dehydrogenase) rise trend of these indexes, and serum and whey SOD (superoxide dismutase, glutathione (GSH-PX) peroxidase), MDA (malondialdehyde), NO (nitric oxide) before and after the administration activity changes of these indexes were detected, a comprehensive understanding of traditional Chinese medicine drug suspension.
In vitro drug sensitivity study showed that the minimum inhibitory concentration (MIC) of licorice flavonoids suspension against Streptococcus lactis was lowest (MBC), and the minimum bactericidal concentration (MBC) was 4.88 g / mL. The MIC and MBC for Staphylococcus aureus were 19.53 g / mL, and the MIC for E. coli was 625 g / mL..
Safety test results show that the traditional Chinese medicine preparation pyretogen accords with the Chinese Pharmacopoeia; the traditional Chinese medicine injection had no hemolysis; do not cause skin allergic reactions, is a weak sensitizer; on rabbit leg muscle stimulation series is less than 2, can be used for intramuscular injection; belong to non-toxic substances on the acute toxicity of mice; no difference of healthy cows breast stimulation the effect of injection with saline (P0.05), the results show that the total flavonoids of Glycyrrhiza suspension injection breast with clinical medication safety.
Dose screening test results show that the Chinese medicine treatment group, 20ml/ 40ml/ group in dairy, dairy group, antibiotics group, the cure rate can be as high as 86%, 89.36%, 86.54%, and the cure rate are not significantly difference between the three groups (P > 0.05), efficiency is as high as 90%, 93.62%, 94.23%, the difference is not significant (P > 0.05); after the treatment by Staphylococcus aureus, Streptococcus agalactiae and no milk in Escherichia coli and mixed bacteria caused in traditional Chinese medicine after treatment negative rate were 70%, 100%, 28.57%, 50%, total bacteria negative rate as high as 69.04%, compared with the antibiotic group difference significant (P > 0.05).
The clinical curative effect of expanding test results show that: Traditional Chinese medicine group and the antibiotic treatment group 60 head sick cows, the cure rate was 87.17%, 86.84%, the efficiency was up to 97.44%, 93.42%; after the final dose, compared with healthy milk somatic cell counts, somatic medicine group and the antibiotic group were restored to the normal range of SCC < 500 thousand, but still the antibiotic group was significantly higher than that of healthy values (P < 0.05); the prevalence of dairy cows after 5D treatment, traditional Chinese medicine group lactation returned to normal value (P0.05); sick cows were cured after whey and serum enzyme glutathione peroxidase, superoxide dismutase activity significantly (P < 0.05), nitric oxide, MDA content decreased significantly (P < 0.05 or P0.01), compared with the normal value of no difference in WHEY WHEY albumin, lactate dehydrogenase, N- acetyl beta -D- Glucosaminidase were significantly lower (P < 0.05 or P0.01).
The above research results indicate that licorice total flavonoids suspension breast injections are safe and effective in repairing damaged mammary tissues, which is effective in treating clinical mastitis in dairy cows, providing reliable practice and theoretical basis for the development of new veterinary drugs.

【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.23

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本文編號：1737381