本文選題：卵母細(xì)胞 + 老化��； 參考：《山東農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：卵母細(xì)胞老化過程中氧自由基累積導(dǎo)致線粒體、內(nèi)質(zhì)網(wǎng)等細(xì)胞器功能受損,細(xì)胞內(nèi)Ca2+平衡失調(diào),MPF活性下降,卵母細(xì)胞的激活敏感性升高,胚胎發(fā)育能力下降。自噬是吞噬自身細(xì)胞質(zhì)蛋白或細(xì)胞器并使其包被進(jìn)入囊泡,與溶酶體融合形成自噬溶酶體,降解其所包裹的內(nèi)容物的過程,藉此實(shí)現(xiàn)細(xì)胞本身的代謝需要和某些細(xì)胞器的更新。研究發(fā)現(xiàn)自噬是胚胎發(fā)育所必需的,并且在卵母細(xì)胞體外成熟期間激活自噬能夠提高胚胎發(fā)育能力,但卵母細(xì)胞老化過程中自噬的變化及作用研究較少。因此我們以小鼠為模型,研究體內(nèi)成熟卵母細(xì)胞在體內(nèi)和體外老化過程中自噬的變化規(guī)律,以及自噬對(duì)卵母細(xì)胞老化和發(fā)育的影響。結(jié)果如下：1、小鼠卵母細(xì)胞體內(nèi)老化12h后自噬降低,老化24h后自噬不再下降。2、小鼠卵母細(xì)胞體外老化12h期間在H202條件培養(yǎng)基中培養(yǎng),與對(duì)照組相比自噬下降；添加MG132組自噬則高于對(duì)照組。3、小鼠卵母細(xì)胞體外老化12h期間,添加自噬激活劑Rapamycin,自噬增加,激活率下降,胚胎發(fā)育能力升高；而添加自噬抑制劑3-MA,自噬下降,激活率增加,胚胎發(fā)育能力降低。4、小鼠卵母細(xì)胞體外老化12h后自噬降低,老化24h后自噬不再下降。5、小鼠卵母細(xì)胞體外老化0h時(shí)或老化24h時(shí)添加3-MA,降低卵母細(xì)胞碎裂率；而在卵母細(xì)胞老化12h或24h期間添加3-MA,不影響卵母細(xì)胞碎裂。6、小鼠卵母細(xì)胞體外老化期間,添加Rapamycin,不影響卵母細(xì)胞碎裂。總之,卵母細(xì)胞無論在體內(nèi)還是在體外老化過程中自噬下降,老化24h后自噬不再下降。卵母細(xì)胞體外老化12h期間,激活自噬,降低激活率,提高發(fā)育能力；抑制自噬,增加激活率,降低發(fā)育能力。自噬不影響卵母細(xì)胞碎裂。
[Abstract]:During oocyte aging, the accumulation of oxygen free radicals resulted in damage of mitochondria, endoplasmic reticulum and other organelles, decreased the activity of Ca2, increased the sensitivity of oocytes to activation, and decreased the ability of embryonic development.Autophagy is the process of phagocytosis of its own cytoplasmic protein or organelle and its encapsulation into vesicles, fusion with lysosome to form autophagy lysosomes, and degradation of the contents contained therein, thereby realizing the metabolic needs of the cells themselves and the renewal of some organelles.It was found that autophagy is necessary for embryonic development, and activation of autophagy during oocyte maturation in vitro can improve the embryonic development ability, but there is little research on the changes and effects of autophagy during oocyte aging.So we used mouse model to study the changes of autophagy during in vivo and in vitro aging and the effect of autophagy on the aging and development of oocytes.The results were as follows: (1) the autophagy of mouse oocytes decreased after 12h aging and stopped decreasing after 24h aging. The mouse oocytes were cultured in H202 medium during 12h aging in vitro, and the autophagy decreased compared with the control group.During 12h aging of mouse oocytes, the rate of autophagy increased, the rate of autophagy decreased and the ability of embryonic development increased, but the rate of autophagy decreased and the activation rate increased.The development ability of mouse oocytes decreased, the autophagy of mouse oocytes decreased after aging in vitro for 12 h, and the autophagy did not decrease at 24 h after aging. The rate of oocyte fragmentation was reduced by adding 3-MAat at 0 h or 24 h after aging in vitro.The addition of 3-MAduring 12h or 24h of oocyte aging did not affect the cleavage of oocytes. However, during the aging of mouse oocytes in vitro, Rapamycin was added and oocyte fragmentation was not affected.In short, the autophagy of oocytes decreased during in vivo and in vitro aging, and no longer decreased after 24 h aging.During 12h aging in vitro, oocytes activated autophagy, decreased activation rate and increased developmental ability, inhibited autophagy, increased activation rate and decreased developmental ability.Autophagy does not affect oocyte fragmentation.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S814

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李國東;吳德全;李本義;;細(xì)胞自噬在腫瘤中作用的研究進(jìn)展[J];癌癥;2009年04期

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本文編號(hào)：1737201