本文選題：microRNA-a(miR-a)　切入點：牛　出處：《黑龍江畜牧獸醫(yī)》2017年21期

【摘要】：為了探討miR-181a對牛骨骼肌衛(wèi)星細胞分化的影響,試驗將分離培養(yǎng)的牛骨骼肌衛(wèi)星細胞接種在6孔板中,待細胞匯合度為70%時,用PEI轉(zhuǎn)染miR-181a的模擬物miR-181a-M、抑制劑miR-181a-I、模擬物對照miR-181a-M-NC和抑制劑對照miR-181a-I-NC進入牛骨骼肌衛(wèi)星細胞中,在0,1,2,3天用Western-blot檢測肌肉有關(guān)分化蛋白MyoD、MyoG和MYH3以及靶基因HOX-A11蛋白的表達量,用免疫熒光法檢測細胞肌管的數(shù)量。結(jié)果表明:在牛骨骼肌衛(wèi)星細胞中,轉(zhuǎn)染miR-181a模擬物的牛骨骼肌衛(wèi)星細胞,有關(guān)肌肉分化蛋白質(zhì)如MyoD、MyoG和MYH3的表達量明顯增加,細胞肌管數(shù)量顯著增多,進一步證實miR-181a促進細胞的分化。在雙熒光素酶報告系統(tǒng)中,miRNA-181a和HOX-A11基因的3'-UTR結(jié)合時熒光素酶活性明顯下降,miRNA-181a和突變的3'-UTR結(jié)合時熒光素酶活性沒有下降。說明HOX-A11是miR-181a的靶基因,miR-181a對牛骨骼肌衛(wèi)星細胞的分化具有促進作用。
[Abstract]:In order to investigate the effect of miR-181a on the differentiation of bovine skeletal muscle satellite cells, the isolated and cultured bovine skeletal muscle satellite cells were inoculated in a 6-well plate.鐢≒EI杞煋miR-181a鐨勬ā鎷熺墿miR-181a-M,鎶戝埗鍓俶iR-181a-I,妯℃嫙鐗╁鐓iR-181a-M-NC鍜屾姂鍒跺墏瀵圭収miR-181a-I-NC榪涘叆鐗涢楠艱倢鍗槦緇嗚優(yōu)涓，

本文編號：1712466