本文選題：偽狂犬病毒　切入點：分離　出處：《中國獸醫(yī)學報》2017年03期

【摘要】：2011年以來,我國多個省份免疫過偽狂犬病疫苗的規(guī)�；i場發(fā)生新生仔豬出現(xiàn)神經(jīng)癥狀和死亡的現(xiàn)象,并已確定為豬偽狂犬病病毒(pseudorabies virus,PRV)變異株感染所引起。為探究現(xiàn)PRV流行株特性,2014年下半年以來,我們從免疫過偽狂犬疫苗卻暴發(fā)偽狂犬病的豬場分離到3株偽狂犬病病毒(分別為XP,QJ,LY株),并對其gE、gB、gC全基因進行測序和序列分析,序列比對結(jié)果顯示,3株P(guān)RV分離株與2012年后分離毒株有較高的同源性;進化樹分析顯示,3株P(guān)RV分離株的gE基因與2012年后分離毒株處在同一進化樹分支上,而gB、gC則分別形成了獨立的小分支,但仍處在同一較大分支上。我們對當?shù)貜V泛使用的疫苗株HB-98和Bartha-K61活疫苗免疫血清與Ea,HNX株以及3株P(guān)RV分離株進行中和試驗,結(jié)果顯示,HB-98和Bartha-K61活疫苗免疫血清對早期分離株Ea株的中和能力高于HNX、XP、QJ、LY株,但在NX、XP、QJ、LY株之間無明顯差異,表明3株P(guān)RV分離株的抗原性與2012年后分離毒株無明顯差異,而與傳統(tǒng)毒株Ea株的抗原性存在一定差異。
[Abstract]:Since 2011, neurologic symptoms and death in newborn piglets have occurred in large scale pig farms immunized with pseudorabies virus in many provinces of China, and have been identified as caused by the infection of pseudorabies virus (PRV) variant strain of pseudorabies virus (PRV).The results of sequence alignment showed that there was a high homology between the three PRV isolates and the strains isolated after 2012, and the analysis of evolutionary tree showed that the GE gene of the three PRV isolates was in the same branch of the evolutionary tree as that of the virus isolates isolated after 2012.On the other hand, gBN GC formed independent small branches, but still in the same larger branch.鎴戜滑瀵瑰綋鍦板箍娉涗嬌鐢ㄧ殑鐤嫍鏍狧B-98鍜孊artha-K61媧葷柅鑻楀厤鐤娓呬笌Ea,HNX鏍互鍙，

本文編號：1706066