本文選題：仔豬均勻度　切入點：仔豬初生重　出處：《中國農業(yè)科學院》2015年碩士論文

【摘要】：研究顯示,仔豬均勻度對養(yǎng)豬業(yè)有極大影響,血清皮質醇及胰島素含量可能與仔豬的初生重相關。本研究采用動物模型REML(約束最大似然)方法對大白豬8個繁殖性狀的遺傳參數進行估計,利用SAS9.1統計軟件的GLM程序分析年份、配種季節(jié)、胎次對8個繁殖性狀的影響,采用Duncan,s法進行各固定效應間繁殖性狀的多重比較;采用放射免疫分析法檢測不同初生重仔豬血清皮質醇含量及胰島素含量。旨在為育種工作提供仔豬均勻度遺傳參數數據支持;為探索皮質醇及胰島素對初生重的影響起參考作用。1.仔豬均勻度遺傳參數估計利用5種統計模型對大白豬窩內仔豬初生重變異系數(CV0)、窩內仔豬21日齡重變異系數(CV3)、總產仔數(TNB)、產活仔數(NBA)、產死仔數(NBD)、初生窩重(TLW0)、21日齡窩重(TLW3)及妊娠周期(GL)進行遺傳參數估算。經比較,應用模型V(包括個體加性遺傳效應,母體遺傳效應、永久環(huán)境效應、與配公豬效應、母豬所在窩效應)得到的殘差方差所占比例小。因此本實驗采用模型V進行繁殖性狀遺傳參數估計。遺傳參數估計結果表明:TNB,NBA,NBD,CV0,CV3,TLW0,TLW3,GL遺傳力分別為0.14,0.13,0.07,0.07,0.02,0.11,0.08,0.09。TNB與NBA、TNB與TLW0、TNB與TLW3、NBA與TLW0、NBA與TLW3、CV0與CV3、TLW0與TLW3之間存在明顯的遺傳正相關(遺傳相關分別為0.93,0.61,0.61,0.65,0.73,0.65,0.91);TNB與CV0、TNB與CV3、CV0與NBD之間遺傳相關分別為0.29,0.17,0.22。固定效應方差分析表明:年份對TNB和CV0影響不顯著(P0.05),對其余性狀的影響都達到極顯著(P0.01);配種季節(jié)對TNB、NBA、CV3影響不顯著(P0.05),對CV0影響達顯著水平(P0.05),對TLW0,TLW3和GL影響達到極顯著水平(P0.01);胎次對對TNB、NBA、NBD的影響達顯著水平(P0.05),對CV0、CV3、TLW0、TLW3及GL影響極顯著(P0.01)。產仔數對所有性狀影響都達到極顯著水平(P0.01)。繁殖性狀最小二乘均值表明:隨著胎次增加,TNB、NBA、TLW0、TLW3呈現出相同的變化規(guī)律,第一胎最低,以后逐漸上升,到3-4胎時候達到最大,然后又開始下降。季節(jié)對繁殖性狀的影響表現為冬季生產性能最佳;隨著產仔數的增加,CV0、CV3、NBD也增加,而MBW0、MBW3、GL卻下降;隨著產仔數增加,初生重小于1 Kg仔豬所占比例增加,體重大于1.8 Kg仔豬所占比例下降。2.血清皮質醇和胰島素含量水平與仔豬初生重相關性分析實驗選取10窩大白豬,每窩包括2頭低初生重(1.0 Kg)2頭高初生重(1.2 Kg),共40頭初生仔豬作為實驗樣本。仔豬出生后前腔靜脈采血,離心后提取血清,采用放射免疫分析法檢測血清中皮質醇含量及胰島素含量。研究結果表明:高出生重組和低出生重組血清皮質醇量分別為214.72(ng/ml),267.87(ng/ml),兩組之間差異極顯著(P=0.0083);高出生重組和低出生重組血清胰島素含量分別為28.67(m IU/L),20.92(m IU/L),兩組間差異顯著(P=0.0284)。血清皮質醇含量與初生體重之間存在顯著負相關(rg=-0.32),血清胰島素含量與體重之間存在正相關(rg=0.23),但是二者之間相關性不顯著(P=0.09)。實驗結果為探索皮質醇及胰島素對初生重的影響提供參考。
[Abstract]:Research shows that piglet uniformity to the pig industry has great influence, and the birth weight of piglets serum cortisol and insulin content. This study used the animal model of REML (restricted maximum likelihood) genetic parameters of 8 reproductive traits in large white pigs estimation method, analysis of the year, the use of SAS9.1 statistical software GLM program of breeding season. Effect of parity on 8 reproductive traits by using Duncan, s multiple comparison method of reproductive traits of fixed effects; radioimmunoassay was used to measure the different birth weight of piglets serum cortisol and insulin content. In order to provide piglets for breeding data uniformity and genetic parameters of support; to explore the effects of insulin and cortisol the birth weight of the piglets reference.1. uniformity of genetic parameter estimation of large white nest piglets weight variation coefficient using 5 statistical model (CV0), wo Zi 21 day old pig weight coefficient of variation (CV3), total litter size (TNB), number born alive (NBA), produce litter (NBD), birth weight (TLW0), litter weight at 21 days (TLW3) and pregnancy period (GL) of genetic parameter estimation. By comparison, application the V model (including individual additive genetic effects, maternal genetic effect and permanent environmental effect, and with the boar sows effect, where litter effect) residual variance obtained small proportion. Therefore this experiment adopts the V model to reproduce the genetic parameters estimation of genetic parameters of traits. The results showed that: TNB, NBA, NBD, CV0. CV3, TLW0, TLW3, GL and 0.14,0.13,0.07,0.07,0.02,0.11,0.08,0.09.TNB respectively, heritability of NBA, TNB and TLW0, TNB and TLW3, NBA and TLW0, NBA and TLW3, CV0 and CV3, there were significant genetic positive correlation between TLW0 and TLW3 (genetic correlation = 0.93,0.61,0.61,0.65,0.73,0.65,0.91); TNB and CV0, TNB and CV3, between CV0 and NBD genetic correlation respectively. Show that the 0.29,0.17,0.22. variance analysis of fixed effects: the effect of TNB and CV0 years (P0.05), no significant effects on other traits were at significant (P0.01); breeding season on TNB, NBA, CV3 had no significant effects on CV0 (P0.05), significant effects (P0.05), TLW0, TLW3 and GL influence reached a significant level (P0.01); parity on to TNB, NBA, NBD significant effects (P0.05), CV0, CV3, TLW0, TLW3 and GL had significant effect (P0.01). Litter size were all highly significant for all traits affecting reproductive traits (P0.01) showed that the least squares mean. With the increase of TNB, NBA, parity, TLW0, TLW3 showed the same variation, the first minimum, after rising to 3-4 when the tire reaches the maximum, and then began to decline. The seasonal effect on reproductive traits for winter production best; with the increase of litter size of CV0, CV3, NBD increased, while MBW0, M BW3, GL decreased; with the increase of litter size, birth weight less than 1 Kg increase in the proportion of piglets, piglets weight greater than 1.8 Kg the proportion of.2. analysis of serum cortisol and insulin levels and birth weight of the piglets correlation experiment selected 10 large white nest down, including 2 low birth weight per litter (1 Kg) 2 heads the birth weight (1.2 Kg), a total of 40 newborn piglets as the experimental samples. The piglets born after blood samples were collected after centrifugation, extract serum, cortisol and insulin content in serum was measured by radioimmunoassay. The analysis results show that the high born restructuring and low birth weight were 214.72 recombinant serum cortisol (ng/ml) (ng/ml, 267.87), significant differences between the two groups (P=0.0083); high birth and low birth of recombinant insulin in recombinant were 28.67 (m IU/L), 20.92 (m, IU/L) between the two groups were significant difference (P=0.0284). The content of serum cortisol There was a significant negative correlation between birth weight and body weight (rg=-0.32). There was a positive correlation between serum insulin and body weight (rg=0.23), but the correlation between the two groups was not significant (P=0.09). The results of the experiment provide a reference for exploring the influence of cortisol and insulin on the birth weight.

【學位授予單位】：中國農業(yè)科學院
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S828

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