本文選題：肉牛　切入點(diǎn)：黃體期和卵泡期　出處：《中國(guó)獸醫(yī)學(xué)報(bào)》2016年01期

【摘要】：利用PCR-DGGE技術(shù)明確肉牛陰道菌群結(jié)構(gòu)并比較黃體期和卵泡期肉牛陰道菌群結(jié)構(gòu)差異。選擇7頭肉牛,分別采集黃體期和卵泡期陰道分泌物,提取細(xì)菌總DNA,采用巢式PCR分別對(duì)細(xì)菌16SrDNA的V3區(qū)進(jìn)行擴(kuò)增,將PCR產(chǎn)物進(jìn)行變性梯度凝膠電泳(denatured gradient gel electrophoresis,DGGE)。對(duì)DNA指紋圖譜特異性條帶切膠回收并進(jìn)行克隆測(cè)序,通過(guò)BLAST與已知序列對(duì)比,鑒定細(xì)菌菌種,分析比較黃體期和卵泡期肉牛陰道菌群結(jié)構(gòu)。結(jié)果顯示:健康肉牛陰道內(nèi)存在陰道氣球菌(Aerococcus vaginalis str.)、綠色氣球菌(Aerococcusviridans str.)、睡眠嗜血桿菌(Haemophilussomnus str.)、多動(dòng)物鏈球菌(Streptococcus pluranimalium str.)、嗜冷桿菌(Psychrobactermarincolastr.)、大腸桿菌(Escherichia coli O157:H7str.)和鞘氨醇單胞菌(Sphingomonasroseiflavastr.)等。
[Abstract]:The structure of vaginal flora of beef cattle was determined by PCR-DGGE technique and the differences between luteal phase and follicular phase were compared.Seven beef cattle were selected to collect vaginal secretions from luteal phase and follicular stage, and total bacterial DNA was extracted. The V3 region of bacterial 16SrDNA was amplified by nested PCR, and the PCR product was denatured gradient gel electrophoresism DGGEN by denaturing gradient gel electrophoresis.The specific band cut gum of DNA fingerprint was recovered and cloned and sequenced. The bacterial species were identified by comparing BLAST with known sequences and the structure of vaginal flora of beef cattle in luteal phase and follicular stage was analyzed and compared.The results showed that in the vagina of healthy beef cattle, there were Aerococcus vaginalis str., Aerococcus viridans str., Haemophilus somnus str., Streptococcus pluranimalium str., Psychrobacter sp., Escherichia coli O157: H7str.)And Sphingomonas sphingomonas rosei flavastr.Etc.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;
【基金】：吉林省現(xiàn)代肉牛產(chǎn)業(yè)技術(shù)體系項(xiàng)目(201534) 吉林省重大科技攻關(guān)專項(xiàng)(20140203016NY) 吉林省發(fā)改委產(chǎn)業(yè)技術(shù)研發(fā)專項(xiàng)(2013779) 國(guó)家自然科學(xué)基金資助項(xiàng)目(31172227)
【分類號(hào)】：S852.61

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