本文選題：甲醛　切入點(diǎn)：綿羊　出處：《黑龍江八一農(nóng)墾大學(xué)》2017年碩士論文

【摘要】：由各種原因引起的綿羊腹瀉病是當(dāng)今養(yǎng)羊業(yè)發(fā)展的巨大障礙。本試驗(yàn)將大慶市周邊某羊場(chǎng)的爆發(fā)性腹瀉病病例作為切入點(diǎn),研究甲醛口服液對(duì)綿羊腹瀉病的治療效果。選擇健康綿羊5只灌服甲醛口服液做安全試驗(yàn);另選健康綿羊5只,腹瀉綿羊12只做治療試驗(yàn)。在治療試驗(yàn)中選2只腹瀉綿羊直接屠宰做病理組織切片檢查,剩余10只綿羊,平均分為2組,每組5只,對(duì)照組采用羊場(chǎng)常規(guī)治療即肌注恩諾沙星,試驗(yàn)組進(jìn)行灌服5.6 m L甲醛口服液治療,健康組正常飼養(yǎng)。三組試驗(yàn)羊于試驗(yàn)期第1 d、2 d、4 d頸靜脈采血,檢測(cè)RBC、HCT、HGB、MCV、WBC、Seg neutr、Band neutr、Mon、Lym、Eos血液生理指標(biāo)和ALB、ALKP、ALT、GGT、GLU、TP、CREA、BUN血液生化指標(biāo);試驗(yàn)期第1 d、2 d、4 d、6d采集綿羊糞便檢測(cè)性狀、寄生蟲、白細(xì)胞、紅細(xì)胞、上皮細(xì)胞、淀粉顆粒、真菌等指標(biāo)。試驗(yàn)完畢后,三組中各選擇一只綿羊屠宰,取皺胃和十二指腸組織樣本,應(yīng)用HE染色法對(duì)其黏膜形態(tài)結(jié)構(gòu)、炎性細(xì)胞數(shù)量變化等進(jìn)行檢測(cè)。試驗(yàn)結(jié)果:(1)綜合綿羊發(fā)病情況、臨床癥狀、剖檢變化、細(xì)菌分離鑒定及動(dòng)物試驗(yàn)等,確定本次羊場(chǎng)爆發(fā)的綿羊腹瀉病病原為致病性大腸桿菌。(2)安全試驗(yàn)中,綿羊灌服甲醛口服液后,各項(xiàng)血液生理、生化指標(biāo)變化不明顯,糞便檢查未見(jiàn)異常。(3)治療試驗(yàn)中,試驗(yàn)第1 d,試驗(yàn)組與健康組相比,WBC、Band neutr含量急劇上升且差異極顯著(P0.01),治療后第4 d含量有所降低,差異不顯著(P0.05);試驗(yàn)1 d時(shí),試驗(yàn)組和對(duì)照組綿羊的Lym較健康組有所降低,其中,試驗(yàn)組與對(duì)照組處于同一水平差異不顯著(P0.05),而試驗(yàn)組與健康組相比差異顯著(P0.05),第4 d時(shí)差異不顯著(P0.05);試驗(yàn)組綿羊第1 d的MCV與健康組相比較,平均值有所升高且差異極顯著(P0.01),與對(duì)照組相比差異顯著(P0.05),第2 d平均值稍有降低但與健康組相比較差異顯著(P0.05),與對(duì)照組差異不顯著(P0.05),第4 d時(shí),試驗(yàn)組與其他兩組比較,差異均不顯著(P0.05);生化指標(biāo)中試驗(yàn)組與健康組比較,GLU含量第1 d和第2 d時(shí)有所降低,且差異顯著(P0.05),第4 d時(shí)差異不顯著(P0.05);試驗(yàn)組綿羊血液中TP含量第1 d時(shí)與健康組相比較,有所升高且差異顯著(P0.05),第2 d、4 d時(shí)試驗(yàn)組與其他兩組比較差異均不顯著(P0.05);與健康組的糞檢結(jié)果相比較,試驗(yàn)組糞便第4 d時(shí)稀便全部轉(zhuǎn)為干便,對(duì)照組仍存在稀便且有炎性細(xì)胞存在。綜上所述,5.6 m L甲醛口服液對(duì)健康綿羊無(wú)毒害作用,對(duì)綿羊大腸桿菌性腹瀉有治療效果,且相同天數(shù)時(shí),灌服甲醛口服液的試驗(yàn)組治療效果優(yōu)于恩諾沙星治療的對(duì)照組。
[Abstract]:Sheep diarrhea caused by various reasons is a great obstacle to the development of sheep industry.In this study, the effect of formaldehyde oral solution on sheep diarrhea was studied by taking the case of outbreak diarrhea in a sheep farm around Daqing as the cut-in point.5 healthy sheep were given formaldehyde oral solution for safety test, 5 healthy sheep and 12 diarrhea sheep were selected for treatment test.In the treatment experiment, 2 diarrhea sheep were slaughtered directly for histopathological examination. The remaining 10 sheep were divided into 2 groups on average, 5 in each group. The control group was treated with routine treatment of sheep farm, that is, enrofloxacin was injected intramuscularly.The experimental group was treated with 5.6 mL formaldehyde oral solution, and the healthy group was fed normally.Starch granules, fungi and other indicators.After the experiment, one sheep was slaughtered in each group, and the samples of abomasum and duodenum were collected. The morphological structure of mucosa and the number of inflammatory cells were detected by HE staining.Results: (1) synthesizing the incidence, clinical symptoms, dissecting changes, bacteria isolation and identification and animal tests of sheep, confirmed that the pathogen of sheep diarrhea disease in this sheep farm was pathogenic Escherichia coli.After the sheep was given formaldehyde oral liquid, the changes of blood physiological and biochemical indexes were not obvious, and the fecal examination was not abnormal.On the first day of the experiment, the content of neutr in the test group and the control group increased sharply and the difference was very significant (P 0.01). The content of Lym in the test group and the control group was lower than that in the healthy group on the 4th day after treatment, and the Lym of the test group and the control group was lower than that of the healthy group.There was no significant difference between the test group and the control group at the same level (P 0.05), but the difference between the test group and the healthy group was significant (P 0.05), and there was no significant difference at the 4th day (P 0.05). The MCV of the test group was compared with that of the healthy group on the first day.The content of GLU in the test group was lower than that in the healthy group on the 1st and 2nd day, and there was no significant difference between the test group and the healthy group on the 4th day, and the TP content in the blood of the test group was compared with that of the healthy group on the first day.There was no significant difference between the test group and the other two groups on the second day after 4 days, and compared with the results of fecal examination in the healthy group, the feces in the test group turned to dry stool at the 4th day, and there was no significant difference between the two groups on the 4th day after the treatment, compared with the results of fecal examination in the healthy group, the feces in the test group turned to dry stool.In the control group, there were still dilute stool and inflammatory cells.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.26

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