本文選題：黃芩苷　切入點(diǎn)：馬立克氏病病毒(MDV)　出處：《中國家禽》2017年01期

【摘要】：探討黃芩苷體外抗馬立克氏病病毒(MDV)作用,應(yīng)用細(xì)胞病變觀察法(CPE)觀察MDV對(duì)雞胚成纖維細(xì)胞(CEF)的影響,采用Reed-Muench法計(jì)算MDV細(xì)胞半數(shù)感染量(TCID50),以CEF為宿主細(xì)胞,應(yīng)用四甲基偶氮唑藍(lán)(MTT)比色法從黃芩苷對(duì)MDV的增殖抑制、感染阻斷以及直接滅活3個(gè)給藥途徑探討黃芩苷抗MDV作用。結(jié)果顯示:MDV對(duì)CEF的TCID50為10-4.5/0.1 m L;3種給藥途徑下,黃芩苷濃度在312.5~78.13μg/m L范圍都具有較好的抗MDV感染作用,并且感染阻斷途徑下黃芩苷濃度為312.5~156.25μg/m L時(shí)能夠促進(jìn)細(xì)胞的生長,直接滅活途徑下黃芩苷濃度為312.5μg/m L時(shí)能夠促進(jìn)細(xì)胞的生長。其中感染阻斷與直接滅活途徑給藥比增值抑制途徑給藥的抗MDV感染作用效果要好,直接殺滅途徑給藥時(shí)的病毒抑制率最高。
[Abstract]:To investigate the effect of baicalin on Marek's disease virus (MDV) in vitro, to observe the effect of MDV on chicken embryo fibroblast (CEF) by cytopathic observation (CPE), to calculate the half infective quantity of MDV cells (TCID50) by Reed-Muench method, and to use CEF as host cell, and to investigate the effect of baicalin on Marek's disease virus (MDV) in vitro. The effects of baicalin on MDV proliferation inhibition, infection blocking and direct inactivation were studied by tetramethylazolyl methylene tetrazolium (MTT) colorimetric method. The results showed that the TCID50 of CEF was 10-4.5 / 0.1 mL 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路min ~ (-1) 路min ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1). Baicalin concentration in the range of 78.13 渭 g / mL from 312.5 to 78.13 渭 g / mL had a good anti-infection effect on MDV, and baicalin concentration of 312.5 渭 g / mL could promote the growth of the cells under the infection blocking pathway, and the concentration of baicalin was 156.25 渭 g / mL, and the concentration of baicalin was 156.25 渭 g / mL. Baicalin concentration of 312.5 渭 g / mL could promote cell growth in the direct inactivation pathway. The effect of infection blocking and direct inactivation on MDV infection was better than that of value-added inhibition. The virus inhibition rate was the highest when the drug was administered directly.
【作者單位】： 聊城大學(xué)農(nóng)學(xué)院;
【基金】：山東省自然科學(xué)基金項(xiàng)目(ZR2013CL010)
【分類號(hào)】：S852.65

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本文編號(hào)：1691944