本文選題：新城疫病毒　切入點：病毒樣顆粒　出處：《中國獸醫(yī)學報》2017年08期

【摘要】：新城疫病毒樣顆粒(NDV VLPs)是由基質(zhì)蛋白(M)為病毒骨架,裝配血凝素-神經(jīng)氨酸酶和/或融合蛋白等。已有研究表明,NDV VLPs能誘導機體產(chǎn)生特異性體液和細胞免疫應答。然而,關于NDV VLPs如何激活天然免疫應答的研究尚無報道。樹突狀細胞(DCs)作為專職抗原遞呈細胞,在連接天然免疫與適應性免疫之間具有獨特功能。本試驗以NDV VLPs與小鼠DCs為研究靶點,將NDV VLPs(M+HN)刺激DC以評價DC成熟表征。結果顯示該顆�？杀籇C有效吞噬并遞呈給初始型T細胞,誘導DC表面MHCⅡ和共刺激分子顯著上調(diào)以及促進DC分泌促炎性細胞因子。另外,還發(fā)現(xiàn)不同組裝類型的NDV VLPs對誘導DC分泌炎性細胞因子水平不一。結果表明,NDV VLPs可誘導DC成熟,這對更好地理解VLPs激活的天然免疫應答奠定基礎,并且為優(yōu)選NDV VLPs候選株提供參考依據(jù)。
[Abstract]:NDV-like particles of Newcastle disease virus (NDVVLPs) are composed of matrix protein (M) as viral skeleton, which assembles hemagglutinin-neuraminidase and / or fusion proteins. It has been shown that NDV-like VLPs can induce specific humoral and cellular immune responses. There are no reports on how NDV VLPs activates innate immune response. Dendritic cells (DC), as specialized antigen presenting cells, have unique function in linking innate and adaptive immunity. NDV VLPs and mouse DCs were used as targets in this study. NDV VLPs(M HN) was used to stimulate DC to evaluate DC maturation characterization. The results showed that the granules could be effectively phagocytized by DC and presented to initial T cells, induce MHC 鈪，

本文編號：1680937