本文選題：苦豆子總堿和苦參堿制劑　切入點：UV法　出處：《內(nèi)蒙古農(nóng)業(yè)大學》2015年碩士論文

【摘要】：苦豆子總堿和苦參堿制劑具有清熱解毒,消腫止痛和殺蟲等廣泛藥理活性和藥用價值,臨床應用于抗病毒、抗腫瘤、抗寄生蟲試驗的研究,調(diào)結(jié)體內(nèi)免疫系統(tǒng)、心血管系統(tǒng)、中樞神經(jīng)系統(tǒng)。本試驗中苦豆子總堿和苦參堿來自鄂托克前旗,采摘的苦豆子經(jīng)內(nèi)蒙古農(nóng)業(yè)科學院獸醫(yī)研究所研制苦豆子總堿和苦參堿制劑。通過UV法和HPLC法對苦豆子總堿和苦參堿制劑進行影響因素試驗進行穩(wěn)定性試驗,恒溫加速試驗測定藥物保質(zhì)期。為苦豆子的開發(fā)應用及保存有重要意義。本研究得出的結(jié)論如下：(1)成功用UV法和HPLC法構(gòu)建了苦豆子總堿和苦參堿制劑的測定方法,兩種方法測定的結(jié)果差異顯著,得到較為理想的結(jié)果,然后并用兩種方法對兩種藥物的初始濃度進行穩(wěn)定性試驗、精密度測定、重復性測定、加樣回收試驗,比較兩種方法的RSD值。HPLC法測定的RSD值都在0.1-0.2之間,而UV法測定的RSD值在0.8-5.04之間,說明HPLC法測定結(jié)果要精確,HPLC法比UV法測定方法重現(xiàn)性好,靈敏度好,精密度高。(2)文章第二部分以起始濃度為基準。在不同光照和溫度下,用UV法和HPLC法對苦豆子總堿和苦參堿濃度進行測定,并觀察顏色變化,測定兩種藥物的PH值。應用SAS9.0的GLM過程進行方差分析,不同溫度下,4℃時濃度降低最慢,45℃濃度降低的最快。在不同光照下,冷熒光燈中30001x濃度最慢,紫外光下濃度降低的最快。兩種方法測定的藥物濃度均有差異。(3)本研究成功用兩種方法測定苦豆子總堿和苦參堿恒溫加速實驗,HPLC法測定苦豆子總堿保質(zhì)期為1年,UV法測定苦豆子總堿保質(zhì)期為1.9.HPLC方法測得苦參堿保質(zhì)期為2.7年。UV.法測定苦參堿保質(zhì)期為2.1年。
[Abstract]:The preparation of total alkaloids and matrine has a wide range of pharmacological activities and medicinal value, such as clearing heat and detoxification, reducing swelling and relieving pain and killing insects. It has been used in the study of antiviral, anti-tumor and anti-parasitic tests, in regulating the immune system in vivo and in cardiovascular system. Central nervous system. In this experiment, total alkaloids and matrine came from the former flag of Otok. The total alkaloids and matrine preparations were prepared by the Veterinary Research Institute of Inner Mongolia Academy of Agricultural Sciences. The stability tests of total alkaloids and matrine preparations were carried out by UV and HPLC methods. It is of great significance for the development, application and preservation of Sophora alopecuroides to determine the shelf life of the drug. The conclusions of this study are as follows: 1) the method for the determination of total alkaloids and matrine preparations has been successfully constructed by UV and HPLC methods. The difference between the two methods was significant and the results were satisfactory. Then the initial concentration of the two drugs was tested for stability, precision measurement, reproducibility test, sample recovery test, and then two methods were used to test the initial concentration of the two drugs. The RSD value of the two methods was 0.1-0.2, and the RSD value of UV method was 0.8-5.04, which indicated that the results of HPLC method were more reproducible and sensitive than that of UV method. The second part of the paper is based on the initial concentration. Under different light and temperature, the total alkaloids and matrine concentrations were determined by UV and HPLC methods, and the color changes were observed. The pH value of the two drugs was measured. The analysis of variance using the GLM process of SAS9.0 showed that the slowest decrease of concentration at 4 鈩，

本文編號：1680249