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雙內(nèi)源指示劑法測(cè)定兔飼糧消化能值及其預(yù)測(cè)模型的研究

發(fā)布時(shí)間:2018-03-26 10:54

  本文選題:內(nèi)源指示劑 切入點(diǎn):兔飼糧 出處:《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2017年07期


【摘要】:本試驗(yàn)旨在研究利用雙內(nèi)源指示劑法測(cè)定兔飼糧養(yǎng)分消化率及消化能(DE)值,并建立兔飼糧DE值的預(yù)測(cè)模型。選用體重相近的4月齡獺兔30只,隨機(jī)分為3組,每組10個(gè)重復(fù),每個(gè)重復(fù)1只兔。每組飼喂1種飼糧,用全收糞法進(jìn)行消化試驗(yàn),用聚酯纖維篩網(wǎng)袋法測(cè)定了飼糧中的2種內(nèi)源指示劑,即酸不溶木質(zhì)素(ADL)和鹽酸不溶灰分(AIA)+ADL的回收率。同時(shí)選取了山東省不同飼料公司生產(chǎn)的17種兔顆粒配合飼料,在各自兔養(yǎng)殖場(chǎng)進(jìn)行飼養(yǎng)試驗(yàn),利用雙內(nèi)源指示劑法測(cè)定兔飼糧DE值,建立兔飼糧DE值的預(yù)測(cè)模型。結(jié)果表明:1)全收糞法測(cè)得3種飼糧ADL的平均回收率為99.87%,ADL+AIA的平均回收率為96.81%,二者可作為理想的雙內(nèi)源指示劑。2)利用雙內(nèi)源指示劑法可以準(zhǔn)確測(cè)定兔飼糧的各種養(yǎng)分消化率和DE值。3)兔飼糧DE值可以用飼糧中可消化有機(jī)物質(zhì)(DOM)含量進(jìn)行準(zhǔn)確的預(yù)測(cè):DE(M J/kg)=0.0204DOM(g/kg)或DE(M J/kg)=0.939 5+0.018 2DOM(g/kg)。4)利用兔飼糧中養(yǎng)分[粗蛋白質(zhì)(CP)、粗脂肪(EE)、可溶性碳水化合物(NDSC)、半纖維素(HCEL)和纖維素(CEL)]含量建立兔飼糧DE值的預(yù)測(cè)模型為DE(MJ/kg)=0.016 6CP(g/kg)+0.0334EE(g/kg)+0.015 3NDSC(g/kg)+0.005 4HCEL(g/kg)+0.003 1CEL(g/kg)。由此可見(jiàn),利用雙內(nèi)源指示劑法可以較好地預(yù)測(cè)兔飼糧的DE值。
[Abstract]:The purpose of this study was to study the determination of dietary nutrient digestibility and digestibility (DED) of rabbits by double endogenous indicator method, and to establish a prediction model of dietary DE value. Thirty rabbits of 4 months old with similar body weight were randomly divided into 3 groups with 10 repeats in each group. Each rabbit was fed with one diet, digestible test was conducted with the method of total feces collection, and two kinds of endogenous indicators in the diet were determined by the method of polyester fiber sieve net bag. The recovery rate of acid-insoluble lignin (ADL) and hydrochloric acid insoluble ash (AIAA) ADL was studied. Seventeen kinds of rabbit pellet formulated feed produced by different feed companies in Shandong Province were selected and fed in their respective rabbit farms. The dietary DE value of rabbits was determined by double endogenous indicator method. A prediction model of dietary DE value in rabbits was established. The results showed that the average recovery rate of ADL in three diets was 99.87%, and the average recovery rate of AIA was 96.81%, which could be used as the ideal double endogenous indicator. 2) the double endogenous indicator method was used. The digestibility and DE value of various nutrients in rabbit diet can be accurately measured. 3) the dietary DE value of rabbits can be accurately predicted by the digestible organic matter (DOM) content in diet. The nutrient [crude protein] [crude protein] in rabbit diet can be used in the use of 0.0204 DOM / kg / kg or DE(M J/kg)=0.939 5 0.018 mg / kg / kg 路4). The prediction model of dietary DE value of rabbits was established as DE(MJ/kg)=0.016 6 CPG / kg) 0.0334EESCG / kg) 0.015 3NDSCG / kg) 0.005 4HCELG / kg) 0.003 1CEL / kg / kg. Thus, it can be seen that the prediction model of dietary DE value of rabbits is: DE(MJ/kg)=0.016 6CPG / kg) 0.0334EESCG / kg / kg) 0.005 4HCELg / kg) 0.003 1CEL / kg / kg. The double endogenous indicator method can be used to predict the DE value of rabbit diet.
【作者單位】: 山東農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;
【基金】:現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專(zhuān)項(xiàng)(CARS-44-B-1) 山東農(nóng)業(yè)大學(xué)青年科技創(chuàng)新基金項(xiàng)目(2015-2016);山東農(nóng)業(yè)大學(xué)博士后科學(xué)基金(2015-2017) 中國(guó)博士后科學(xué)基金(2015M58061)
【分類(lèi)號(hào)】:S829.1

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