本文選題：八眉豬　切入點(diǎn)：煙臺(tái)黑豬　出處：《浙江農(nóng)業(yè)學(xué)報(bào)》2017年06期

【摘要】：為了研究豬Nramp1基因內(nèi)含子6的多態(tài)性與仔豬腹瀉的相關(guān)性,采用PCR-RFLP法對(duì)八眉豬和煙臺(tái)黑豬Nramp1基因內(nèi)含子6的多態(tài)性進(jìn)行研究。結(jié)果表明:兩個(gè)豬種在該基因內(nèi)含子6的多態(tài)性較低,共檢測(cè)到兩個(gè)等位基因(A、B)和兩種基因型(AB、BB),八眉豬多態(tài)信息含量(PIC)為0.33,煙臺(tái)黑豬為0.36�？ǚ竭m合性檢驗(yàn)顯示,兩豬種的Nramp1基因內(nèi)含子6極顯著偏離Hardy-Weinberg平衡(P0.01)。相關(guān)性分析結(jié)果表明,八眉豬對(duì)腹瀉的抵抗能力顯著高于煙臺(tái)黑豬(P0.05)。性別與Nramp1基因內(nèi)含子6基因型對(duì)仔豬腹瀉影響不顯著(P0.05),而性別與基因型的互作對(duì)仔豬腹瀉有顯著影響(P0.05),在母豬群體中,AB基因型的腹瀉評(píng)分極顯著大于BB基因型(P0.01);在公豬群體中,AB和BB基因型腹瀉評(píng)分差異不顯著(P0.05)。
[Abstract]:To study the association between the polymorphism of intron 6 of Nramp1 gene and diarrhea in piglets. The polymorphism of intron 6 of Nramp1 gene in Bamei pig and Yantai black pig was studied by PCR-RFLP method. Two alleles were detected and two genotypes, the polymorphic information content (Pi) of Bamei pig and Yantai black pig were 0.33 and 0.36 respectively. Chi-square fitness test showed that intron 6 of Nramp1 gene of two breeds deviated from Hardy-Weinberg equilibrium P0.01a significantly. The resistance ability of Bamei pig to diarrhea was significantly higher than that of Yantai black pig P0.05.The sex and Nramp1 gene intron 6 genotype had no significant effect on diarrhea of piglets, while the interaction of sex and genotype had significant effect on diarrhea of piglets, especially in sows. The diarrhea score of genotype B was significantly higher than that of genotype BB (P 0.01), but there was no significant difference in diarrhea score between genotype AB and genotype BB in boar population (P 0.05).
【作者單位】： 西北民族大學(xué)生命科學(xué)與工程學(xué)院;
【基金】：西北民族大學(xué)中央學(xué)�；究蒲袠I(yè)務(wù)費(fèi)專(zhuān)項(xiàng)資金資助項(xiàng)目(31920170029);西北民族大學(xué)本科生科研創(chuàng)新項(xiàng)目(URIP16196)
【分類(lèi)號(hào)】：S858.28
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本文編號(hào)：1666391