發(fā)布時間：2018-03-24 15:50

  本文選題：牦牛　切入點：瘤胃液　出處：《草業(yè)學(xué)報》2017年06期

【摘要】：本研究旨在從西藏地區(qū)牦牛瘤胃中分離出具有分解纖維素能力的兼性厭氧菌,為青貯飼料微生物添加劑的研制提供支持。試驗采集了西藏那曲地區(qū)成年牦牛的新鮮瘤胃液,經(jīng)剛果紅染色初篩,濾紙降解復(fù)篩,獲得分解纖維素能力強(qiáng)的菌株,對菌株生長速率及酶活力進(jìn)行測定,經(jīng)形態(tài)學(xué)、生理生化特性和16SrRNA序列分析對菌株進(jìn)行鑒定,并分析了菌株對水稻秸稈的降解效果。試驗分離出一株兼性厭氧纖維素降解菌,屬于腸球菌屬,即糞腸球菌(Enterococcus faecalis)JF85。菌株JF85在15~55℃,pH 3.0~7.0及3.0%和6.5%NaCl培養(yǎng)基中生長良好。接種JF85,36h后可獲得最大內(nèi)切葡聚糖酶活力(0.41U/mL)和濾紙酶活力(0.13U/mL)。在模擬發(fā)酵試驗中,接種菌株JF85,14d后水稻秸稈干物質(zhì)、纖維素和半纖維素含量與對照組相比顯著降低(P0.05);在發(fā)酵第7天,JF85處理組顯示最高的可溶性碳水化合物含量,整個發(fā)酵過程中木質(zhì)素含量變化不顯著(P0.05)。菌株JF85具有分解纖維素、耐酸、耐鹽特性,在青貯飼料生產(chǎn)中具有較好的應(yīng)用前景。
[Abstract]:The purpose of this study was to isolate facultative anaerobes with cellulolysis ability from the rumen of yak in Tibet, and to provide support for the development of microbial additives for silage. The fresh rumen fluid of adult yaks in Naqu region, Tibet, was collected. The strain with strong ability to decompose cellulose was obtained by primary screening with Congo red staining and double screening by filter paper degradation. The growth rate and enzyme activity of the strain were determined. The strains were identified by morphological, physiological and biochemical characteristics and 16SrRNA sequence analysis. A facultative anaerobic cellulose degrading strain was isolated, which belongs to Enterococcus. That is, Enterococcus faecalisus JF85.The strain JF85 grew well in the medium of pH 3.0, 7.0 and 3.0% and 6.5%NaCl at 1555 鈩，

本文編號：1658978