本文選題：豬關(guān)節(jié)炎　切入點(diǎn)：豬鏈球菌　出處：《上海交通大學(xué)》2015年碩士論文

【摘要】：豬關(guān)節(jié)炎(porcine arthritis)是指由一種或多種因素造成的關(guān)節(jié)組織炎癥,通常由細(xì)菌性病原引起。據(jù)研究,我國(guó)大部分豬場(chǎng)關(guān)節(jié)炎主要由豬鏈球菌(streptococcus suis,S.suis),副豬嗜血桿菌(haemophilus parasuis,HPS)和葡萄球菌(Staphylococcus)等病原菌引起,給豬養(yǎng)殖業(yè)帶來(lái)了極大損失。S.suis和HPS還能引起豬其他的臨床癥狀,且由于長(zhǎng)期應(yīng)用抗生素,導(dǎo)致病原菌表現(xiàn)出多重耐藥性,給豬場(chǎng)疾病防治造成了很大困難。為了解我國(guó)華東地區(qū)部分豬場(chǎng)豬關(guān)節(jié)炎主要病原體的流行狀況,本文根據(jù)鏈球菌2型(streptococcus suis type 2,S.suis2)的莢膜多糖基因cps2J和HPS的16s rRNA基因建立了檢測(cè)豬鏈球菌2型和副豬嗜血桿菌的二重PCR檢測(cè)方法,利用該方法,對(duì)2013-2014年期間華東地區(qū)部分豬場(chǎng)疑似豬關(guān)節(jié)炎的120份細(xì)菌樣品進(jìn)行檢測(cè)鑒定,獲得14株S.suis2和12株HPS。在120份細(xì)菌樣品中檢測(cè)到21株S suis,表明S.suis2是鏈球菌中主要流行菌株,并通過(guò)進(jìn)一步建立的系列多重PCR方法證明S.suis分離株的毒力因子分布型為低毒力型:+cps2J-/+mrp-ef-sly-gdh-/+gadph-/+orf2。將14株S.suis2的莢膜多糖基因cps2J與四株國(guó)內(nèi)株和兩株國(guó)外株進(jìn)行序列比對(duì),結(jié)果顯示同源性為94%—99%,說(shuō)明S.suis2分離株的cps2J基因發(fā)生了變異,基因進(jìn)化樹(shù)分析表明華東地區(qū)S.suis2具有廣域性;對(duì)4株HPS分離株進(jìn)行tbpA基因的限制性片段多態(tài)性(restriction fragment length polymorphism,RFLP)分型分析,結(jié)果表明4株HPS都為副豬嗜血桿菌桿菌最流行的DBE型。對(duì)S.suis和HPS分離株進(jìn)行藥敏試驗(yàn),發(fā)現(xiàn)兩種菌表現(xiàn)多重耐藥性,并且青霉素類(lèi)類(lèi)和頭孢菌素類(lèi)藥物對(duì)兩者均有較好的敏感性,提示青霉素類(lèi)藥物和頭孢菌素類(lèi)藥物可作為豬關(guān)節(jié)炎的治療藥物。本研究首先為檢測(cè)豬場(chǎng)關(guān)節(jié)病主要病原提供了快速準(zhǔn)確的檢測(cè)方法,檢測(cè)結(jié)果為華東地區(qū)豬關(guān)節(jié)病病原學(xué)研究積累了有益資料,對(duì)病原菌主要毒力因子的初步分析可為未來(lái)疫苗的研制提供參考,藥敏試驗(yàn)也可為制定豬關(guān)節(jié)病防治措施提供一定的指導(dǎo)。
[Abstract]:Porcine arthritis is an inflammation of the articular tissue caused by one or more factors, usually caused by bacterial pathogens. Most of the arthritis in pig farms in China is mainly caused by pathogenic bacteria such as streptococcus suis S.suis, Haemophilus parahaemophilus parasuisus HPSs and Staphylococcus staphylococcus, which have brought great losses to pig breeding. S. suis and HPS can also cause other clinical symptoms in pigs. As a result of long-term application of antibiotics, the pathogens showed multidrug resistance, which caused great difficulties in the prevention and treatment of pig farm diseases, in order to understand the prevalence of the main pathogens of pig arthritis in some pig farms in East China. Based on the capsule polysaccharide gene cps2J of Streptococcus suis type 2 and 16s rRNA gene of HPS, a double PCR method for detection of Haemophilus suis and Streptococcus suis 2 was established. From 2013 to 2014, 120 samples of suspected porcine arthritis from pig farms in East China were detected and identified, 14 strains of S.suis2 and 12 strains of HPSS were obtained. 21 strains of S suis were detected in 120 samples of bacteria, indicating that S.suis2 is the main prevalent strain of streptococcus. A series of multiplex PCR methods were established to prove that the virulence factor distribution of S.suis isolates was low virulence type: cps2J-/ mrp-ef-sly-gdh-/ gadph-/ orf2.The sequence of cps2J gene of 14 S.suis2 isolates was compared with four domestic and two foreign strains. The results showed that the homology was 94-99, which indicated that the cps2J gene of S.suis2 isolates had been mutated, and the phylogenetic tree analysis of S.suis2 in East China showed that S.suis2 was widespread, and four HPS isolates were analyzed by restriction fragment length polymorphism (RFLP). The results showed that the four strains of HPS were the most popular DBE type of Haemophilus parasuis. The drug sensitivity test of S.suis and HPS isolates showed that the two strains showed multidrug resistance. Penicillin and cephalosporins have good sensitivity to both. It is suggested that penicillin and cephalosporins can be used as therapeutic drugs for porcine arthritis. The results provided useful data for the etiological study of swine arthropathy in East China, and the preliminary analysis of the main virulence factors of pathogenic bacteria could provide a reference for the development of vaccine in the future. The drug sensitivity test can also provide some guidance for the prevention and treatment of pig arthropathy.
【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S858.28

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 宋德平;肖根輝;王萍;何后軍;鄔向東;黃冬艷;;副豬嗜血桿菌江西株的分離鑒定及生物學(xué)特性研究[J];江西農(nóng)業(yè)大學(xué)學(xué)報(bào);2011年04期

2 熊蕊;劉曉慧;左玉柱;范京惠;郭鳳柳;趙同欣;王娜;顏紅;;副豬嗜血桿菌感染豬的臨床癥狀及病理學(xué)觀(guān)察[J];科技致富向?qū)?2014年02期

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本文編號(hào)：1656839