本文選題：滸苔多糖　切入點(diǎn)：犬冠狀病毒　出處：《中國獸醫(yī)學(xué)報(bào)》2017年09期 　論文類型：期刊論文

【摘要】：采用水煮醇沉法提取滸苔多糖,用PMP柱前衍生高效液相色譜串聯(lián)質(zhì)譜儀測滸苔多糖組成成分和含量,在犬冠狀病毒(canine coronavirus,CCV)滅活苗中添加不同劑量的滸苔多糖,制備犬冠狀病毒多糖滅活疫苗。選取30只40日齡的健康土狗隨機(jī)分成6組,每組5只,Ⅰ~Ⅳ組為滸苔多糖疫苗組(滸苔多糖含量為10,20,30,40g/L),Ⅴ組為滸苔多糖對照組(滸苔多糖含量為30g/L),Ⅵ組為不含滸苔多糖組,各組分別于免疫后7,14,21,35,42,49,56d采血,進(jìn)行犬冠狀病毒抗體效價(jià)、淋巴細(xì)胞比率、淋巴細(xì)胞轉(zhuǎn)化率、IL-2和IFN-γ各指標(biāo)檢測。結(jié)果表明,分離純化后滸苔多糖含量為69.2%,滸苔多糖有6種成分,主要是鼠李糖和木糖;Ⅰ~Ⅴ組各指標(biāo)均顯著高于Ⅵ組(P0.05),Ⅳ組各指標(biāo)顯著高于其他各組(P0.05)。由此表明滸苔多糖可以顯著提高犬對犬冠狀病毒滅活疫苗的免疫作用,滸苔多糖用量對疫苗免疫作用有影響,這為滸苔多糖作為免疫增強(qiáng)劑的開發(fā)奠定基礎(chǔ)。
[Abstract]:The polysaccharides of Enteromorpha enteromorpha were extracted by boiling alcohol precipitation method. The composition and content of polysaccharides of Enteromorpha enteromorpha were determined by PMP precolumn derivatization high performance liquid chromatography series mass spectrometer, and different doses of polysaccharides were added to the inactivated vaccine of canine coronavirus. of canine coronavirus. To prepare inactivated canine coronavirus polysaccharide vaccine, 30 healthy indigenous dogs aged 40 days were randomly divided into 6 groups, 5 dogs in each group. Groups 鈪，

本文編號：1650048