本文選題：生物電　切入點：鹿茸　出處：《農業(yè)生物技術學報》2017年07期 　論文類型：期刊論文

【摘要】：近年來,生物電編碼動物/動物器官形態(tài)發(fā)生成為生命科學研究領域的一個里程碑式成果。迄今為止對該領域的研究僅局限于低等動物。為了初步探索哺乳動物器官的形態(tài)發(fā)生與生物電的相關性。本研究以梅花鹿(Cervus nippon)鹿茸為模型,選擇進入青春期前的1歲齡仔鹿(鹿茸尚未開始發(fā)育),雌性和雄性仔鹿各3頭;通過外科手術摘取鹿茸形態(tài)發(fā)生原胚生茸區(qū)骨膜(antlerogenic periosteum,AP)組織共10片,利用杜爾伯科改良伊格爾培養(yǎng)基(Dulbecco's modified eagle medium,DMEM)培養(yǎng)基(含10%胎牛血清和2%的青霉素-鏈霉素)進行離體培養(yǎng);分別將培養(yǎng)液中加入4種細胞離子通道干擾藥物(ivermectin,MS-222,concanamycin A和SCH-28080)對AP組織進行了干擾;連續(xù)培養(yǎng)2.5 d。結果發(fā)現,不管是使用去極化藥物還是超極化藥物處理均對鹿茸的形態(tài)發(fā)育產生了一定程度的影響。為了驗證藥物的作用效果,本研究對AP細胞進行了離體培養(yǎng),并選用MS-222作為代表對AP細胞進行了處理。通過Coro Na染色,發(fā)現MS-222降低了AP細胞的細胞內鈉離子的濃度;通過3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽(3-(4,5)-dimethylthiahiazo(-z-y1)-2,5-di-phenytetrazoliumromide,MTT)實驗發(fā)現,MS-222沒有影響AP細胞的增殖率。說明MS-222之類的藥物對AP細胞并不具有細胞毒性,因而其抑制鹿茸發(fā)育的最可能的途徑是通過對細胞離子通道的干擾影響了AP組織的極化狀態(tài)。本研究結果表明,AP組織離子通道的狀態(tài)與鹿茸的發(fā)育之間可能存在直接關系。因此推測,鹿茸可能與低等動物模型一樣,只有特定的生物電狀態(tài)才能維持正常的形態(tài)發(fā)生。本研究為進一步驗證生物電編碼哺乳動物器官形態(tài)發(fā)生選定的動物模型提供了理論依據。
[Abstract]:In recent years, Bioelectric coding of animal / animal organ morphogenesis has become a milestone in the field of life sciences. So far, the research in this field is limited to lower animals. In this study, Cervus nippon (Cervus nippon) velvet antler was used as a model. A total of 10 pieces of tissue were selected from 1 year old baby deer before puberty (antlerogenic peristeum APs), which had not yet begun to develop, 3 male and 3 female, and were surgically removed from antlerogenic peristeum of antler morphogenetic antellogenic antler. In vitro culture was carried out on Dulbeccodes modified eagle medium (containing 10% fetal bovine serum and 2% penicillin streptomycin). Four kinds of cell channel interfering drugs, MS-222concanamycin A and SCH-28080, were added to the culture medium respectively, and cultured continuously for 2.5 days. Both depolarizing and hyperpolarizing drugs had a certain effect on the morphological development of velvet antler. In order to verify the effect of the drugs, the AP cells were cultured in vitro. MS-222 was used as the representative to treat AP cells. Through Coro Na staining, it was found that MS-222 decreased the intracellular sodium concentration of AP cells. The results of the 3-trimethylthiazole-5-dimethylthiahiazot-5-dimethylthiahiazo-z-y1ttrium 5-di-phenyzoliumromide (MTT) assay showed that MS-222 did not affect the proliferation rate of AP cells, indicating that the drugs such as MS-222 were not cytotoxic to AP cells, and the results showed that MS-222 did not affect the proliferation rate of AP cells, which indicated that MS-222 was not cytotoxic to AP cells, and the results showed that MS-222 did not affect the proliferation rate of AP cells, and the results showed that MS-222 did not affect the proliferation rate of AP cells. Therefore, the most likely way to inhibit the development of velvet antler is to influence the polarization state of AP tissue by interfering with the cell ion channel. The results of this study indicate that there may be a relationship between the ion channel state of AP tissue and the development of pilose antler. Direct relationship. Therefore, conjecture, Velvet antler may be the same as the lower animal model, only a specific bioelectric state can maintain normal morphogenesis. This study provides a theoretical basis for further verification of the selected animal model of bioelectric coding mammalian organ morphogenesis.
【作者單位】： 中國農業(yè)科學院特產研究所/特種經濟動物分子生物學國家重點實驗室/吉林省鹿茸工程研究中心;
【基金】：吉林省重點科技攻關項目(No.20150204071NY)
【分類號】：S825

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